In-line system containing porous polymer monoliths for protein digestion with immobilized pepsin, peptide preconcentration and nano-liquid chromatography separation coupled to electrospray ionization mass spectroscopy

Journal of Chromatography A

Volumn 1188, Issue 2, 2008, Pages 88-96

  Geiser, Laurent ^a   Eeltink, Sebastiaan ^a   Svec, Frantisek ^b   Fréchet, Jean M J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

Enzymatic reactor; Immobilized enzyme; Mass spectrometry; Monolithic columns; NanoHPLC; Pepsin

Indexed keywords

ENZYMATIC REACTORS; MONOLITHIC COLUMNS; PEPSIN; PROTEIN DIGESTION;

ELECTROSPRAY IONIZATION; ENZYME IMMOBILIZATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDES; POROUS MATERIALS;

POLYMERS;

2 VINYL 4,4 DIMETHYLAZLACTONE; ALBUMIN; ETHYLENE GLYCOL DIMETHACRYLATE; HEMOGLOBIN; IMMOBILIZED ENZYME; LACTONE DERIVATIVE; METHACRYLIC ACID DERIVATIVE; MYOGLOBIN; PEPSIN A; PEPTIDE; POLY(LAURYL METHACRYLATE CO ETHYLENE DIMETHACRYLATE); UNCLASSIFIED DRUG;

ARTICLE; BIOREACTOR; CONCENTRATION (PARAMETERS); DILUTION; ELECTROSPRAY MASS SPECTROMETRY; POLYMERIZATION; POROSITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; TIME OF FLIGHT MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, LIQUID; ENZYMES, IMMOBILIZED; HEMOGLOBINS; LACTONES; MICROSCOPY, ELECTRON, SCANNING; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MYOGLOBIN; NANOTECHNOLOGY; ONLINE SYSTEMS; PEPSIN A; PEPTIDES; POLYMERS; POROSITY; PROTEINS; SOLUTIONS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 41549110915     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2008.02.075     Document Type: Article

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