Automated in-solution protein digestion using a commonly available high-performance liquid chromatography autosampler

Analytical Biochemistry

Volumn 411, Issue 2, 2011, Pages 284-291

  Richardson, Jason ^a   Shah, Bhavana ^a   Xiao, Gang ^a   Bondarenko, Pavel V ^a   Zhang, Zhongqi ^a  

^a AMGEN INC   (United States)

Author keywords

Automation; Autosampler; Digestion; Mass spectrometry; Monoclonal antibody; Peptide mapping; Protein

Indexed keywords

ALKYLATION; AUTOMATION; COVALENT BONDS; DRUG PRODUCTS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUIDS; MAPPING; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; PEPTIDES; PROTEINS; SLUDGE DIGESTION; SULFUR COMPOUNDS;

AUTOMATED PROCEDURES; AUTOSAMPLERS; FORMULATION DEVELOPMENT; LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY; MONOCLONAL ANTIBODIES (MABS); PEPTIDE MAPPING; POST-TRANSLATIONAL MODIFICATIONS; PROTEIN CHARACTERIZATION;

QUALITY CONTROL;

IMMUNOGLOBULIN G2; MONOCLONAL ANTIBODY;

ALKYLATION; ARTICLE; AUTOMATION; DISULFIDE BOND; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN PROCESSING; REDUCTION; TANDEM MASS SPECTROMETRY;

EID: 79952455801     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.01.019     Document Type: Article

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