Virulence genes in the pathogenic yeast Candida albicans

FEMS Microbiology Reviews

Volumn 25, Issue 2, 2001, Pages 245-268

  Navarro García, Federico ^a   Sánchez, Miguel ^b   Nombela, César ^a   Pla, Jesús ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b UNIVERSITY OF SALAMANCA   (Spain)

Author keywords

Candida albicans; Dimorphism; Gene disruption; Pathogenic fungus; Virulence assay

Indexed keywords

GENE PRODUCT; PROTEIN ADE2; PROTEIN BGL2; PROTEIN CHS2; PROTEIN CHS3; PROTEIN CSP37; PROTEIN FAS2; PROTEIN FET3; PROTEIN FTR1; PROTEIN FTR2; PROTEIN GNA1; PROTEIN HEM3; PROTEIN HIS1; PROTEIN HWP1; PROTEIN LEU2; PROTEIN MIG1; PROTEIN MNT1; PROTEIN NMT1; PROTEIN NTC1; PROTEIN PHR1; PROTEIN PHR2; PROTEIN PMT1; PROTEIN PMT6; PROTEIN RBT1; PROTEIN SNF1; PROTEIN TPS1; PROTEIN URA3; UNCLASSIFIED DRUG; UNINDEXED DRUG; WNT1 PROTEIN;

ANIMAL CELL; ANIMAL MODEL; ANIMAL TISSUE; CANDIDA ALBICANS; FEMALE; GENE DISRUPTION; GENE ISOLATION; MALE; MOUSE; NONHUMAN; RELIABILITY; REVIEW; SIGNAL TRANSDUCTION; STRAIN DIFFERENCE; VIRULENCE;

ANIMALS; CANDIDA ALBICANS; CELL WALL; FUNGAL PROTEINS; GENES, FUNGAL; HUMANS; SIGNAL TRANSDUCTION; TRANSCRIPTION, GENETIC; VIRULENCE;

CANDIDA ALBICANS; FUNGI;

EID: 0035105152     PISSN: 01686445     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-6445(00)00066-8     Document Type: Review

References (184)

1. Fungal infection rates are increasing
2. Opportunistic mycosis in the immunocompromissed host: Experience at a cancer center and review
3. Secular trends in nosocomial primary bloodstream infections in the United States. 1980-1989 National Nosocomial Infections Surveillance System
4. Major trends in the microbial etiology of nosocomial infection
5. Evolución de la prevalencia de las infecciones nosocomiales en los hospitales españoles
6. Bloodstream infections due to Candida species: SENTRY antimicrobial surveillance program in North America and Latin America, 1997-1998
7. Nosocomial bloodstream infections in United States hospitals: A three-year analysis
8. Antimicrobial agents: Antifungal agents
9. Antifungal therapy in AIDS patients
10. Resistance of Candida species to fluconazole
11. Mechanisms of resistance to azole antifungal agents in Candida albicans isolates from AIDS patients involve specific multidrug transporters
12. Antifungal drug resistance in pathogenic fungi
13. Candida species and virulence
14. Putative virulence factors of Candida albicans
15. Candida albicans hyphal formation and virulence: Is there a clearly defined role?
16. Antifungal agents: Chemotherapeutic targets and immunological strategies
17. Fungal virulence genes as targets for antifungal chemotherapy
18. Candida albicans - Do mycelia matter?
19. Which came first: The hypha or the yeast?
20. Candida pathogenesis: Unravelling the threads of infection
21. Virulence factors of medically important fungi
22. Molecular Koch's postulates applied to microbial pathogenicity
23. Understanding Candida albicans at the molecular level
24. Genetics of Candida albicans
25. The molecular genetics of Candida albicans
26. Molecular genetics of human fungal pathogens
27. Isogenic strain construction and gene mapping in Candida albicans
28. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains
29. Direct selection of galactokinase-negative mutants of Candida albicans using 2-deoxy-galactose
30. PHR1, a pH-regulated gene of Candida albicans, is required for morphogenesis
31. Sequential gene disruption in Candida albicans by FLP-mediated site-specific recombination
32. Cloning, analysis and one-step disruption of the ARG5, 6 gene of Candida albicans
33. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions
34. A single-transformation gene function test in diploid Candida albicans
35. Induction of mating in Candida albicans by construction of MTLa and MTLα strains
36. Evidence for mating of the 'asexual' yeast Candida albicans in a mammalian host
37. Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration
38. The topoisomerase I gene from Candida albicans
39. Control of filament formation in Candida albicans by the transcriptional repressor TUP1
40. Sequence and promoter regulation of the PCK1 gene encoding phosphoenolpyruvate carboxykinase of the fungal pathogen Candida albicans
41. Efg1p, an essential regulator of morphogenesis of the human pathogen Candida albicans, is a member of a conserved class of bHLH proteins regulating morphogenetic processes in fungi
42. Control of white-opaque phenotypic switching in Candida albicans by the Efg1p morphogenetic regulator
43. Misexpression of the white-phase-specific gene WH11 in the opaque phase of Candida albicans affects switching and virulence
44. Misexpression of the opaque-phase-specific gene PEP1 (SAP1) in the white phase of Candida albicans confers increased virulence in a mouse model of cutaneous infection
45. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae
46. Tetracycline-regulatable system to tightly control gene expression in the pathogenic fungus Candida albicans
47. Isolation of the Candida albicans gene for orotidine-5′-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations
48. Overexpression of a cloned IMP dehydrogenase gene of Candida albicans confers resistance to the specific inhibitor mycophenolic acid
49. Targeted gene disruption in Candida albicans wild-type strains: The role of the MDR1 gene in fluconazole resistance of clinical Candida albicans isolates
50. Pathogenicity of Candida albicans auxotrophic mutants in experimental infections
51. A hyphal-specific chitin synthase gene (CHS2) is not essential for growth, dimorphism, or virulence of Candida albicans
52. Genetic studies reveal that myristoylCoA:protein N-myristoyltransferase is an essential enzyme in Candida albicans
53. Attenuated virulence of chitin-deficient mutants of Candida albicans
54. Reduced virulence of Candida albicans mutants affected in multidrug resistance
55. Role of three chitin synthase genes in the growth of Candida albicans
56. A Candida albicans RAS-related gene (CaRSR1) is involved in budding, cell morphogenesis and hypha development
57. Assessment of a mouse model of neutropenia and the effect of an anti-candidiasis monoclonal antibody in these animals
58. Disruption of each of the secreted aspartyl proteinase genes SAP1, SAP2, and SAP3 of Candida albicans attenuates virulence
59. Acquired resistance and persistence of Candida albicans following oral candidiasis in the mouse: A model of the carrier state in humans
60. Oral and esophageal Candida albicans infection in hyposalivatory rats
61. Avirulence of Candida albicans auxotrophic mutants in a rat model of oropharyngeal candidiasis
62. Requirement for the Candida albicans FAS2 gene for infection in a rat model of oropharyngeal candidiasis
63. Intestinal lesions associated with disseminated candidiasis in an experimental animal model
64. Invasive lesions containing filamentous forms produced by a Candida albicans mutant that is defective in filamentous growth in culture
65. Effect of INT1 gene on Candida albicans murine intestinal colonization
66. A murine model for the study of mycotic mastitis
67. Mitogen-activated protein kinase-defective Candida albicans is avirulent in a novel model of localized murine candidiasis
68. Experimental rat vaginal infection with Candida parapsilosis
69. Experimental chronic vaginal candidosis in rats
70. Contact lens-induced infection - A new model of Candida albicans keratitis
71. Identification and characterization of TUP1-regulated genes in Candida albicans
72. Germ tubes and proteinase activity contribute to virulence of Candida albicans in murine peritonitis
73. Evaluation of the intranasal challenge route in mice as a mucosal model for Candida albicans infection
74. Cellular actin is affected by interaction with Candida albicans
75. Cloning and characterization of CSP37, a novel gene encoding a putative membrane protein of Candida albicans
76. Effect of salivary secretory IgA on the adhesion of candida albicans to polystyrene
77. Altered adherence in strains of Candida albicans harbouring null mutations in secreted aspartic proteinase genes
78. Adherence of Candida albicans to human buccal epithelial cells: Host-induced protein synthesis and signaling events
79. Adhesion of Candida albicans mutant strains to host tissue
80. A multicopper oxidase gene from Candida albicans: Cloning, characterization and disruption
81. Linkage of adhesion, filamentous growth, and virulence in Candida albicans to a single gene, INT1
82. Penetration and damage of endothelial cells by Candida albicans
83. Migration of the fungal pathogen Candida albicans across endothelial monolayers
84. A model of human cutaneous candidosis based on reconstructed human epidermis for the light and electron microscopic study of pathogenesis and treatment
85. Secreted aspartic proteinase (Sap) activity contributes to tissue damage in a model of human oral candidosis
86. Caco-2 monolayer as a model for transepithelial migration of the fungal pathogen Candida albicans
87. Comparative pathogenicity of auxotrophic mutants of Candida albicans
88. Pathogenicity of morphological and auxotrophic mutants of Candida albicans in experimental infections
89. Altered expression of selectable marker URA3 in gene-disrupted Candida albicans strains complicates interpretation of virulence studies
90. The gene encoding phosphoribosylaminoimidazole carboxylase (ADE2) is essential for growth of Cryptococcus neoformans in cerebrospinal fluid
91. Role of the mitogen-activated protein kinase Hog1p in morphogenesis and virulence of Candida albicans
92. Avirulence of Candida albicans FAS2 mutants in a mouse model of systemic candidiasis
93. Disruption of the Candida albicans TPS1 gene encoding trehalose-6-phosphate synthase impairs formation of hyphae and decreases infectivity
94. Trehalose hydrolysis is not required for human serum-induced dimorphic transition in Candida albicans: Evidence from a tps1/tps1 mutant deficient in trehalose synthesis
95. A neutral trehalase gene from Candida albicans: Molecular cloning, characterization and disruption
96. Isolation of the MIG1 gene from Candida albicans and effects of its disruption on catabolite repression
97. A gene homologous to Saccharomyces cerevisiae SNF1 appears to be essential for the viability of Candida albicans
98. Iron acquisition in microbial pathogenesis
99. A high-affinity iron permease essential for Candida albicans virulence
100. Endothelial cell injury caused by Candida albicans is dependent on iron
101. Fluctuations in glycolytic mRNA levels during morphogenesis in Candida albicans reflect underlying changes in growth and are not a response to cellular dimorphism
102. Phenotype in Candida albicans of a disruption of the BGL2 gene encoding a 1,3-beta-glucosyltransferase
103. Phenotypic characterization of a Candida albicans strain deficient in its major exoglucanase
104. Reduced virulence of Candida albicans mutants lacking the GNA1 gene encoding glucosamine-6-phosphate acetyltransferase
105. Multiple functions of Pmt1p-mediated protein O-mannosylation in the fungal pathogen Candida albicans
106. Morphogenesis, adhesive properties, and antifungal resistance depend on the Pmt6 protein mannosyltransferase in the fungal pathogen Candida albicans
107. Molecular analysis of CaMnt1p, a mannosyl transferase important for adhesion and virulence of Candida albicans
108. Molecular analysis of the Candida albicans homolog of Saccharomyces cerevisiae MNN9, required for glycosylation of cell wall mannoproteins
109. PHR2 of Candida albicans encodes a functional homolog of the pH-regulated gene PHR1 with an inverted pattern of pH-dependent expression
110. Defects in assembly of the extracellular matrix are responsible for altered morphogenesis of a Candida albicans phr1 mutant
111. PHR1 and PHR2 of Candida albicans encode putative glycosidases required for proper cross-linking of beta-1,3- and beta-1,6-glucans
112. Reduced virulence of Candida albicans PHR1 mutants
113. The pH of the host niche controls gene expression in and virulence of Candida albicans
114. Developmental expression of a tandemly repeated, proline- and glutamine-rich amino acid motif on hyphal surfaces on Candida albicans
115. Genetic organization and sequence analysis of the hypha-specific cell wall protein gene HWP1 of Candida albicans
116. HWP1 functions in the morphological development of Candida albicans downstream of EFG1, TUP1, and RBF1
117. Adhesive and mammalian transglutaminase substrate properties of Candida albicans Hwp1
118. Reduced virulence of HWP1-deficient mutants of Candida albicans and their interactions with host cells
119. Cloning and expression of a gene encoding an integrin-like protein in Candida albicans
120. Systemic infection following intravenous inoculation of mice with Candida albicans int1 mutant strains
121. Cell wall and secreted proteins of Candida albicans: Identification, function, and expression
122. Growth and guidance of the fungal hypha
123. Isolation and morphological characterization of a mycelial mutant of Candida albicans
124. Isolation and characterization of Candida albicans morphological mutants derepressed for the formation of filamentous hypha-type structures
125. Low virulence of a morphological Candida albicans mutant
126. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: Mating and invasive growth
127. Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth regulation by starvation and RAS
128. Elements of the yeast pheromone response pathway required for filamentous growth of diploids
129. Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae
130. Ras2 signals via the Cdc42/Ste20/mitogen-activated protein kinase module to induce filamentous growth in Saccharomyces cerevisiae
131. Signal transduction through homologs of the Ste20p and Ste7p protein kinases can trigger hyphal formation in the pathogenic fungus Candida albicans
132. Candida albicans strains heterozygous and homozygous for mutations in mitogen-activated protein kinase signaling components have defects in hyphal development
133. Virulence and hyphal formation of Candida albicans require the Ste20p-like protein kinase CaCla4p
134. Constitutive activation of the Saccharomyces cerevisiae mating response pathway by a MAP kinase kinase from Candida albicans
135. Dominant negative selection of heterologous genes: Isolation of Candida albicans genes that interfere with Saccharomyces cerevisiae mating factor-induced cell cycle arrest
136. Identification of a putative transcription factor in Candida albicans that can complement the mating defect of Saccharomyces cerevisiae ste12 mutants
137. Suppression of hyphal formation in Candida albicans by mutation of a STE12 homolog
138. Roles of the Candida albicans mitogen-activated protein kinase homolog, Cek1p, in hyphal development and systemic candidiasis
139. Nonfilamentous C. albicans mutants are avirulent
140. Functional characterization of the MKC1 gene of Candida albicans, which encodes a mitogen-activated protein kinase homolog related to cell integrity
141. A role for the MAP kinase gene MKC1 in cell wall construction and morphological transitions in Candida albicans
142. Reduced virulence of Candida albicans MKC1 mutants: A role for a mitogen-activated protein kinase in pathogenesis
143. Derepressed hyphal growth and reduced virulence in a VH1 family-related protein phosphatase mutant of the human pathogen Candida albicans
144. The mitogen-activated protein kinase homolog HOG1 gene controls glycerol accumulation in the pathogenic fungus Candida albicans
145. Identification of a putative histidine kinase two-component phosphorelay gene (CaHK1) in Candida albicans
146. Flocculation of hyphae is associated with a deletion in the putative CaHK1 two-component histidine kinase gene from Candida albicans
147. Avirulence of Candida albicans CaHK1 mutants in a murine model of hematogenously disseminated candidiasis
148. COS1, a two-component histidine kinase that is involved in hyphal development in the opportunistic pathogen Candida albicans
149. Isolation of CaSLN1 and CaNIK1, the genes for osmosensing histidine kinase homologues, from the pathogenic fungus Candida albicans
150. Roles of three histidine kinase genes in hyphal development and virulence of the pathogenic fungus Candida albicans
151. Identification of a putative response regulator two-component phosphorelay gene (CaSSK1) from Candida albicans
152. Defective hyphal development and avirulence caused by a deletion of the SSK1 response regulator gene in Candida albicans
153. A DNA-binding protein from Candida albicans that binds to the RPG box of Saccharomyces cerevisiae and the telomeric repeat sequence of C. albicans
154. Expression of seven members of the gene family encoding secretory aspartyl proteinases in Candida albicans
155. Multiplicity of genes encoding secreted aspartic proteinases in Candida species
156. The genes encoding the secreted aspartyl proteinases of Candida albicans constitute a family with at least three members
157. Differential regulation of SAP8 and SAP9, which encode two new members of the secreted aspartic proteinase family in Candida albicans
158. Differential expression of secreted aspartyl proteinases in a model of human oral candidosis and in patient samples from the oral cavity
159. In vivo expression and localization of Candida albicans secreted aspartyl proteinases during oral candidiasis in HIV-infected patients
160. Differential activation of a Candida albicans virulence gene family during infection
161. A triple deletion of the secreted aspartyl proteinase genes SAP4, SAP5, and SAP6 of Candida albicans causes attenuated virulence
162. Secreted aspartyl proteinases and interactions of Candida albicans with human endothelial cells
163. Evidence that members of the secretory aspartyl proteinase gene family, in particular SAP2, are virulence factors for Candida vaginitis
164. Possible role of secreted proteinases in Candida albicans infections
165. The expression of the secreted aspartyl proteinases Sap4 to Sap6 from Candida albicans in murine macrophages
166. Candida albicans secreted aspartyl proteases
167. Cloning and disruption of caPLB1, a phospholipase B gene involved in the pathogenicity of Candida albicans
168. Molecular cloning of a second phospholipase B gene, caPLB2 from Candida albicans
169. Cloning and sequencing of a Candida albicans catalase gene and effects of disruption of this gene
170. The expression of the pathogenic yeast Candida albicans catalase gene in response to hydrogen peroxide
171. Ras signaling is required for serum-induced hyphal differentiation in Candida albicans
172. Cloning and characterization of CAD1/AAF1, a gene from Candida albicans that induces adherence to endothelial cells after expression in Saccharomyces cerevisiae
173. Unanticipated heterogeneity in growth rate and virulence among Candida albicans AAF1 null mutants
174. A phosphatidylinositol 3-kinase of Candida albicans influences adhesion, filamentous growth and virulence
175. What is pathogen?
176. Strain-dependent differences in host response to Candida albicans infection in mice are related to organ susceptibility and infectious load
177. Translation elongation factor 2 is encoded by a single essential gene in Candida albicans
178. Overexpression of a dominant-negative allele of SEC4 inhibits growth and protein secretion in Candida albicans
179. Cloning of Candida albicans SEC14 gene homologue coding for a putative essential function
180. Production of pyruvate by Candida albicans: Proposed role in virulence
181. Host-induced, stage-specific virulence gene activation in Candida albicans during infection
182. Natural heterozygosity in Candida albicans
183. Reduced pathogenicity of a Candida albicans MAP kinase phosphatase (CPP1) mutant in the murine mastitis model