Overexpression of a dominant-negative allele of SEC4 inhibits growth and protein secretion in Candida albicans

Journal of Bacteriology

Volumn 181, Issue 23, 1999, Pages 7235-7242

  Mao, Yuxin ^a   Kalb, Vernon F ^a   Wong, Brian ^b  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VA CONNECTICUT HEALTHCARE SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN;

ALLELE; ARTICLE; CANDIDA ALBICANS; FUNGUS GROWTH; GENE OVEREXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SECRETION; SACCHAROMYCES CEREVISIAE;

CANDIDA ALBICANS; SACCHAROMYCES CEREVISIAE;

EID: 0032698991     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.23.7235-7242.1999     Document Type: Article

References (30)

1. Barbacid, M. 1987. Ras genes. Annu. Rev. Biochem. 56:779-827.
2. Burstein, E. S., W. H. Brondyk, and I. G. Macara. 1992. Amino acid residues in the Ras-like GTPase Rab3A that specify sensitivity to factors that regulate the GTP/GDP cycling of Rab3A. J. Biol. Chem. 267:22715-22718.
3. Chibana, H., B. B. Magee, S. Grindle, Y. Ran, S. Scherer, and P. T. Magee. 1998. A physical map of chromosome 7 of Candida albicans. Genetics 149: 1739-1752.
4. Chu, W. S., B. B. Magee, and P. T. Magee. 1993. Construction of an SfiI macrorestriction map of the Candida albicans genome. J. Bacteriol. 175: 6637-6651.
5. Clement, M., H. Fournier, L. D. Repentigny, and P. Belhumeur. 1998. Isolation and characterization of the Candida albicans SEC4 gene. Yeast 14: 675-680.
6. Dunsmuir, P., and M. J. Hynes. 1973. Temperature sensitive mutants affecting the activity and regulation of the acetamidase of Aspergillus nidulans. Mol. Gen. Gen. 123:333-346.
7. Fonzi, W. A., and M. Y. Irwin. 1993. Isogenic strain construction and gene mapping in Candida albicans. Genetics 134:717-728.
8. Gale, C. A., C. M. Bendel, M. McClellan, M. Hauser, J. M. Becker, J. Berman, and M. K. Hostetter. 1998. Linkage of adhesion, filamentous growth, and virulence in Candida albicans to a single gene, INT1. Science 279:1355-1358.
9. Gorman, J. A., W. Chan, and J. W. Gorman. 1991. Repeated use of GAL1 for gene disruption in Candida albicans. Genetics 129:19-24.
10. Goshorn, A. K., S. M. Grindle, and S. Scherer. 1992. Gene isolation by complementation in Candida albicans and applications to physical and genetic mapping. Infect. Immun, 60:876-884.
11. Holz, R. W., W. H. Brondyk, R. A. Senter, L. Kuizon, and I. G. Macara. 1994. Evidence for the involvement of Rab3A in Ca(2+)-dependent exocytosis from adrenal chromaffin cells. J. Biol. Chem. 269:10229-10234.
12. Hube, B., D. Sanglard, F. C. Odds, D. Hess, M. Monod, W. Schafer, A. J. Brown, and N. A. Gow. 1997. Disruption of each of the secreted aspartyl proteinase genes SAP1, SAP2, and SAP3 of Candida albicans attenuates virulence. Infect. Immun. 45:3529-3538.
13. Jones, S., R. J. Litt, C. J. Richardson, and N. Segev. 1995. Requirement of nucleotide exchange factor for Ypt1 GTPase mediated protein transport. J. Cell Biol. 130:1051-1061.
14. Lazar, T., M. Gotte, and D. Gallwitz. 1997. Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? Trends Biochem. Sci. 22:468-472.
15. Leberer, E., K. Ziegelbauer, A. Schmidt, D. Harcus, D. Dignard, J. Ash, L. Johnson, and D. Y. Thomas. 1997. Virulence and hyphal formation of Candida albicans require the Ste20p-like protein kinase CaCla4p. Curr. Biol. 7:539-546.
16. Lodge, J. K., E. Jackson-Machelski, D. L. Toffaletti, J. R. Perfect, and J. I. Gordon. 1994. Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans. Proc. Natl. Acad. Sci. USA 91:12008-12012.
17. MacDonald, D. W., and D. J. Cove. 1972. Studies of temperature-sensitive cnx mutants in the fungus Aspergillus nidulans. Biochem. J. 127:19.
18. Monteoliva, L., M. Sanchez, J. Pla, C. Gil, and C. Nombela. 1996. Cloning of Candida albicans SEC14 gene homologue coding for a putative essential function. Yeast 11:1097-1105.
19. Nieto, A., P. Sanz, R. Sentandreu, and L. del Castillo Agudo. 1993. Cloning and characterization of the SEC18 gene from Candida albicans. Yeast 8:875-887.
20. Novick, P., C. Field, and R. Sheckman. 1980. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21:205-215.
21. Pla, J., R. M. Perez-Diaz, F. Navarro-Garcia, M. Sanchez and C. Nombela. 1995. Cloning of the Candida albicans HIS1 gene by direct complementation of a C. albicans histidine auxotroph using an improved double-ARS shuttle vector. Gene 165:115-120.
22. Pryer, N. K., L. J. Wuestehube, and R. Scheckman. 1992. Vesicle-mediated protein sorting. Annu. Rev. Biochem. 61:471-516.
23. Salminen, A., and P. J. Novick. 1987. A ras-like protein is required for a post-Golgi event in yeast secretion. Cell 49:527-538.
24. Sanglard, D., B. Hube, M. Monod, F. C. Odds, and N. A. Gow. 1997. A triple deletion of the secreted aspartyl proteinase genes SAP4, SAP5, and SAP6 of Candida albicans causes attenuated virulence. Infect. Immun. 65:3539-3546.
25. Tisdale, E. J., J. R. Bourne, R. Khosravi-Far, C. J. Der, and W. E. Balch. 1992. GTP-binding mutants of rab1 and rab2 are potent inhibitors of vesicular transport from the endoplasmic reticulum to the Golgi complex. J. Cell Biol. 119:749-761.
26. Valencia, A., P. Chardin, A. Wittinghofer, and C. Sander. 1991. The ras protein family: evolutionary tree and role of conserved amino acids. Biochemistry 30:4638-4648.
27. Walworth, N. C., B. Goud, A. K. Kabcenell, and P. J. Novick. 1989. Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic. EMBO J. 8:1685-1693.
28. Weinberg, R. A., C. A. McWherter, S. K. Freeman, D. C. Wood, J. I. Gordon, and S. C. Lee. 1995. Genetic studies reveal that myristoylCoA:protein N-myristoyltransferase is an essential enzyme in Candida albicans. Mol. Microbiol. 16:241-250.
29. White, T. C., S. H. Miyasaki, and N. Agablan. 1993. Three distinct secreted aspartyl proteinases in Candida albicans. J. Bacteriol. 175:6126-6133.
30. Zaret, K. S., and F. Sherman. 1982. DNA sequence required for efficient transcription termination in yeast. Cell 28:563-573.