Evidence for mating of the 'asexual' yeast Candida albicans in a mammalian host

Science

Volumn 289, Issue 5477, 2000, Pages 307-310

  Hull, Christina M ^a   Raisner, Ryan M ^a   Johnson, Alexander D ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; CANDIDA ALBICANS; DIPLOIDY; DNA CONTENT; GENE LOCUS; GENETIC RECOMBINATION; MATING; NONHUMAN; PRIORITY JOURNAL; REPRODUCTION; STRAIN DIFFERENCE;

ANIMALS; CANDIDA ALBICANS; CONJUGATION, GENETIC; FEMALE; GENES, FUNGAL; GENES, MATING TYPE, FUNGAL; HOMEODOMAIN PROTEINS; HUMANS; MICE; MULTIGENE FAMILY; PLOIDIES; RECOMBINATION, GENETIC; REPRESSOR PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS;

CANDIDA ALBICANS; FUNGI; MAMMALIA;

EID: 0034647487     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.289.5477.307     Document Type: Article

References (26)

1. F. C. Odds, Candida and Candidosis (Bailliere Tindall, London, ed. 2, 1988).
2. Y. Graser et al., Proc. Natl. Acad. Sci. U.S.A. 93, 12473 (1996).
3. M. Tibayrenc, Trends Microbiol. 5, 253 (1997).
4. R. Vilgalys, Y. Graser, G. Schonion, W. Presber, T. Mitchell, Trends Microbiol. 5, 254 (1997).
5. J. W. Taylor, D. M. Geiser, A. Hurt, V. Koufopanou, Clin. Microbiol. Rev. 12, 126 (1999).
6. C. Sadhu, D. Hoekstra, M. J. McEachern, S. I. Reed, J. B. Hicks, Mol. Cell. Biol. 12, 1977 (1992).
7. E. Leberer et al., Proc. Natl. Acad. Sci. U.S.A. 93, 13217 (1996).
8. M. Raymond et al., Mol. Microbiol. 27, 587 (1998).
9. A. C. Diener and G. R. Fink, Genetics 143, 769 (1996).
10. L. A. Casselton and N. S. Olesnicky, Microbiol. Mol. Biol. Rev. 62, 55 (1998).
11. I. Herskowitz, J. Rine, J. Strathern, in Molecular and Cellular Biology of the Yeast Saccharomyces, E. W. Jones, J. R. Pringle, J. R. Broach, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1992), vol. 2, pp. 583-656.
12. J. W. Kronstad and C. Staben, Annu. Rev. Genet. 31, 245 (1997).
13. B. G. Turgeon, Annu. Rev. Phytopathol. 36, 115 (1998).
14. A. D. Johnson, Curr. Opin. Genet. Dev. 5, 552 (1995).
15. C. M. Hull and A. D. Johnson, Science 285, 1271 (1999).
16. - strains and allowing the cells to retain URA3 after the second round of transformation. Because C. albicans does not contain silent cassettes of MTL (15), the various MTL deletions would be expected to be sufficient to create strains analogous to a and α.
17. 7 yeast cells into the lateral tail vain. Cells for injection were grown to saturation at 30°C and then back-diluted and grown for 4 hours to mid logarithmic phase. The cells were pelleted, resuspended in 0.9% saline, and counted by hemacytometer. Mice were euthanized after 24 hours, and the kidneys were removed, homogenized, and plated in fractions onto standard synthetic medium plus dextrose (SD)-Ade-Ura plates and incubated at 30°C. A fraction of each sample was also plated to medium composed of yeast extract, peptone, plus dextrose (YPD) for total colony counts of recovered cells.
18. C. M. Hull, R. M. Raisner, A. D. Johnson, unpublished data.
19. Data from additional test matings can be found on Science Online (25).
20. Supplemental information on the in vitro conditions tested is available on Science Online (25).
21. Supplemental data and protocol information on the FACS and DAPI analyses are available on Science Online (25).
22. Additional MTL and ADE2 Southern blot information is available on Science Online (25).
23. Supplemental information on the isolation of genomic DNA and Southern blot protocol and probes is available on Science Online (25).
24. Chromosome information was obtained from alces. med.umn.edu/Candida.html. Specific assignments were made by the Stanford Candida albicans sequencing project. Sequence data for C. albicans were obtained from the Stanford DNA Sequencing and Technology Center Web site at www-sequence.stanford.edu/group/ candida.
25. Supplemental data are available at www.sciencemag. org/feature/data/1049869.shl.
26. The authors acknowledge V. Nguyen and additional members of the J. Li laboratory (University of California, San Francisco) for assistance with the FACS analysis, A. Uht, I. Herskowitz D. Ganem, and R. Brazas for helpful comments, and D. Inglis and additional members of the Johnson Laboratory for continuing support and assistance. Sequencing of C. albicans by the Stanford DNA Sequencing and Technology Center was accomplished with the support of the NIDR and the Burroughs Wellcome Fund. This work was supported by NIH grant GM37049 and a Burroughs Wellcome Merit Award to A.D.J.