PHR2 of Candida albicans encodes a functional homolog of the pH- regulated gene PHR1 with an inverted pattern of pH-dependent expression

Molecular and Cellular Biology

Volumn 17, Issue 10, 1997, Pages 5960-5967

  Mühlschlegel, Fritz A ^a   Fonzi, William A ^b  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b GEORGETOWN UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CANDIDA ALBICANS; CELL PH; GENE DELETION; GENE EXPRESSION; MORPHOGENESIS; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; VIRULENCE; YEAST;

CANDIDA ALBICANS;

EID: 1842301080     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.17.10.5960     Document Type: Article

References (29)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Bartl, S., and I. L. Weissman. 1994. PCR primers containing an inosine triplet to complement a variable codon within a conserved protein-coding region. BioTechniques 16:246-250.
3. Birse, C. E., M. Y. Irwin, W. A. Fonzi, and P. S. Sypherd. 1993. Cloning and characterization of ECE1, a gene expressed in association with cell elongation of the dimorphic pathogen Candida albicans. Infect. Immun. 61:3648-3655.
4. Boeke, J. D., F. LaCroute, and G. R. Fink. 1984. A positive selection for mutants lacking orotidine-5′-phosphate decarhoxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet. 197:345-346.
5. Brunt, S., and W. A. Fonzi. Unpublished data.
6. Conzelmann, A., H. Riezman, C. Desponds, and C. Bron. 1988. A major 125-kd membrane glycoprotein of Saccharomyces cerevisiae is attached to the lipid bilayer through an inositol-containing phospholipid. EMBO J. 7:2233-2240.
7. Finney, M. 1994. Molecular cloning of PCR products, p. 15.7.1-15.7.6. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology, vol. 2. Greene Publishing Associates and Wiley Interscience, New York. N.Y.
8. Fonzi, W. A. Unpublished results.
9. Fonzi, W. A., and M. Y. Irwin. 1993. Isogenic strain construction and gene mapping in Candida albicans. Genetics 134:717-728.
10. Fridkin, S. K., and W. R. Jarvis. 1996. Epidemiology of nosocomial fungal infections. Clin. Microbiol. Rev. 9:499-511.
11. Ghannoum, M. A., B. Spellberg, S. M. Saporito-Irwin, and W. A. Fonzi. 1995. Reduced virulence of Candida albicans PHR1 mutants. Infect. Immun. 63: 4528-4530.
12. Gietz, D., A. St. Jean, R. A. Woods, and R. H. Schiestl. 1992. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 20:1425.
13. Gillum, A. M., E. Y. H. Tsay, and D. R. Kirsch. 1984. Isolation of the Candida albicans gene for orotidine-5′-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations. Mol. Gen. Genet. 198:179-182.
14. Kelly, R., S. M. Miller, M. B. Kurtz, and D. R. Kirsch. 1987. Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants. Mol. Cell. Biol. 7:199-208.
15. Li, W., and A. P. Mitchell. 1997. Proteolytic activation of Rim1p, a positive regulator of yeast sporulation and invasive growth. Genetics 145:63-73.
16. Nuoffer, C., P. Jenö, A. Conzelmann, and H. Riezman. 1991. Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane. Mol. Cell. Biol. 11:27-37.
17. Orejas, M., E. A. Espeso, J. Tilburn, S. Sarkar, H. N. Arst, Jr., and M. A. Peñalva. 1995. Activation of the Aspergillus PacC transcription factor in response to alkaline ambient pH requires proteolysis of the carboxy-terminal moiety. Genes Dev. 9:1622-1632.
18. Popolo, L., D. Gilardelli, P. Bonfante, and M. Vai. 1997. Increase in chitin as an essential response to defects in assembly of cell wall polymers in the ggp1Δ mutant of Saccharomyces cerevisiae. J. Bacteriol. 179:463-469.
19. Popolo, L., M. Vai, E. Gatti, S. Porello, P. Bonfante, R. Balestrini, and L. Alberghina. 1993. Physiological analysis of mutants indicates involvement of the Saccharomyces cerevisiae GPI-anchored protein gp115 in morphogenesis and cell separation. J. Bacteriol. 175:1879-1885.
20. Ram, A. F. J., S. S. C. Brekelmans, L. J. W. M. Oehlen, and F. M. Klis. 1995. Identification of two cell cycle regulated genes affecting the β1,3-glucan content of cell walls in Saccharomyces cerevisiae. FEBS Lett. 358:165-170.
21. Ram, A. F. J., and F. M. Klis. 1995. Presented at the Conference on Morphogenesis and Metabolism in Opportunistic Fungal Pathogens, Poitiers, France, 3 to 8 October 1995.
22. Saporito-Irwin, S. M., C. E. Birse, P. S. Sypherd, and W. A. Fonzi. 1995. PHR1, a pH-regulated gene of Candida albicans, is required for morphogenesis. Mol. Cell. Biol. 15:601-613.
23. Sherman, F., G. R. Fink, and J. B. Hicks. 1986. Methods in yeast genetics. Cold Spring Harbor Laboratories, Cold Spring Harbor, N.Y.
24. Su, S. S. Y., and A. P. Mitchell. 1993. Molecular characterization of the yeast meiotic regulatory gene RIM1. Nucleic Acids Res. 21:3789-3797.
25. Tilburn, J., E. A. Sarkar, M. Orejas, J. Mungroo, M. A. Penalva, and H. N. J. Arst. 1995. The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid-and alkaline-expressed genes by ambient pH. EMBO J. 14:779-790.
26. Vai, M., E. Gatti, E. Lacana, L. Popolo, and L. Alberghina. 1991. Isolation and deduced amino acid sequence of the gene encoding gp115, a yeast glycophospholipid-anchored protein containing a serine-rich region. J. Biol. Chem. 266:12242-12248.
27. Vai, M., I. Orlandi, P. Cavadini, L. Alberghina, and L. Popolo. 1996. Candida albicans homologue of GGP1/GAS1 gene is functional in Saccharomyces cerevisiae and contains the determinants for glycosylphosphatidylinositol attachment. Yeast 12:361-368.
28. Vai, M., L. Popolo, R. Grandori, E. Lacana, and L. Alberghina. 1990. The cell cycle modulated glycoprotein GP115 is one of the major yeast proteins containing glycosylphosphatidylinositol. Biochim. Biophys. Acta 1038:277-285.
29. Wickes, B., J. Staudinger, B. B. Magee, K.-J. Kwon-Chung, P. T. Magee, and S. Scherer. 1991. Physical and genetic mapping of Candida albicans: several genes previously assigned to chromosome 1 map to chromosome R, the rDNA-containing linkage group. Infect. Immun. 59:2480-2484.