Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration

Molecular and General Genetics

Volumn 251, Issue 1, 1996, Pages 75-80

  Brown Jr , Douglas H ^a   Slobodkin, Igor V ^a   Kumamoto, Carol A ^a  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Candida albicans URA3; Restriction enzyme mediated integration; Transformation MAL2

Indexed keywords

FUNGAL PROTEIN; RESTRICTION ENDONUCLEASE;

ARTICLE; CANDIDA ALBICANS; CONTROLLED STUDY; DNA INTEGRATION; FUNGAL GENETICS; FUNGUS GROWTH; GENE INDUCTION; GENETIC TRANSFORMATION; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; RECOMBINANT GENE; REPORTER GENE; TRANSCRIPTION REGULATION;

EID: 0029966709     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/bf02174347     Document Type: Article

References (27)

1. Ausubel F, Brent R, Kingston R, Moore D, Seidman J, Smith J, Struhl K (1989) Current protocols in molecular biology. Wiley, New York
2. Bodey G (1988) The emergence of fungi as major hospital pathogens. J Hosp Infect 11 (Suppl A):411-426
3. Cannon RD, Jenkinson HF, Shepard MG (1990) Isolation and nucleotide sequence of an autonomously replicating sequence (ARS) element functional in Candida albicans and Saccharomyces cerevisiae. Mol Gen Genet 221 : 210-218
4. Cannon RD, Jenkinson HF, Shepherd MG (1992) Cloning and expression of Candida albicans ADE2 and proteinase genes on a replicative plasmid in Candida albicans and in Saccharomyces cerevisiae. Mol Gen Genet 235 : 453-457
5. Delbrück S, Ernst J (1993) Morphogenesis-independent regulation of actin transcript levels in the pathogenic yeast Candida albicans. Mol Microbiol 10 : 859-866
6. Fonzi WA, Irwin MY (1993) Isogenic strain construction and gene mapping in Candida albicans. Genetics 134 : 717-728
7. Geber A, Williamson PR, Rex JH, Sweeney EC, Bennet JE (1992) Cloning and characterization of a Candida albicans maltase gene involved in sucrose utilization. J Bacteriol 174 : 6992-6996
8. Gorman JA, Chan W, Gorman JW (1991) Repeated use of GAL1 for gene disruption in Candida albicans. Genetics 129 : 19-24
9. Goshorn AK, Grindle SM, Scherer S (1992) Gene isolation by complementation in Candida albicans and applications to physical and genetic mapping. Infect Immun 60 : 876-884
10. Herreros E, Garcia-Sáez MI, Nombela C, Sánchez M (1992) A reorganized Candida albicans DNA sequence promoting homologous non-integrative genetic transformation. Mol Microbiol 6 : 3567-3574
11. Hong SH, Marmur J (1987) Upstream regulator regions controlling the expression of the yeast maltase gene. Mol Cell Biol 7 : 2477-2483
12. Kelly R, Miller SM, Kurtz MB, Kirsch DR (1987) Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants. Mol Cell Biol 7 : 199-207
13. Kurtz MB, Cortelyou MW, Kirsch DR (1986) Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene. Mol Cell Biol 6 : 142-149
14. Kurtz MB, Cortelyou MW, Miller SM, Lai M, Kirsch DR (1987) Development of autonomously replicating p asmids for Candida albicans. Mol Cell Biol 7 : 209-217
15. Kuspa A, Loomis WF (1992) Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. Proc Natl Acad of Sci USA 89 : 8803-8807
16. Liberman I, Kornberg A, Simms E (1955) Enzymatic synthesis of pyrimidine nucleotides. Orotidine-5′-phosphate and uridine-5′-phosphate. J Biol Chem 215 : 403-415
17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193 : 265-275
18. Miller J (1992) A short course in bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
19. Myers K, Sypherd P, Fonzi W (1995) Use of URA3 as a reporter of gene expression in C. albicans. Curr Genet 27 : 243-248
20. Ni B, Needleman R (1990) Identification of upstream activating sequence of MAL and the binding sites for the MAL63 activator of Saccharomyces cerevisiae. Mol Cell Biol 10 : 3797-3800
21. Pfaller MA (1994) Epidemiology and control of fungal infections. Clin Infect Dis 19(Suppl 1) : S8-S13
22. Ram SP, Sullivan PA, Shepard MG (1983) The in situ assay of Candida albicans enzymes during yeast growth and germ-tube formation. J Gen Microbiol 129 : 2367-2378
23. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
24. Scherer S, Stevens DA (1988) A Candida alhicans dispersed, repeated gene family and its epidemiologic applications. Proc Natl Acad Sci USA 85 : 1452-1456
25. Schiestl RH, Petes TD (1991) Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 88 : 7585-7589
26. Umezu K, Amaya T, Yoshimoto A, Tomita K (1971) Purification and properties of orotidine-5′-phosphate pyrophosphorylase and orotidine-5′-phosphate decarboxylase from bakers' yeast. J Biochem 70 : 249-262
27. Yao B, Marmur J, Sollitti P (1993) Construction of glucose-repressible yeast expression vectors. Gene 137: 223-226