Requirement for the Candida albicans FAS2 gene for infection in a rat model of oropharyngeal candidiasis

Microbiology

Volumn 142, Issue 9, 1996, Pages 2509-2514

  Zhao, Xiao Jiong ^a   McElhaney Feser, Gail E ^a   Bowen, William H ^b   Cole, Michael F ^a   Broedel Jr , Sheldon E ^c   Cihlar, Ronald L ^a  

^a GEORGETOWN UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF ROCHESTER   (United States)

^c ChekTec Corporation   (United States)

Author keywords

Avirulence; Candida albicans; FAS2; Null mutants; Oropharyngeal

Indexed keywords

ANTIFUNGAL AGENT; FATTY ACID SYNTHASE;

ALLELE; AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CANDIDA ALBICANS; CANDIDIASIS; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INACTIVATION; GENE DELETION; GENE MUTATION; MOUTH CAVITY; MYCOSIS; NEWBORN; NONHUMAN; OROPHARYNX; PRIORITY JOURNAL; RAT; SOUTHERN BLOTTING; VIRULENCE;

ANIMALIA; CANDIDA ALBICANS;

EID: 0029763496     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-142-9-2509     Document Type: Article

References (27)

1. Becker, J. M., Henry, L. K., Jiang, W. & Koltin, Y. (1995). Reduced virulence of Candida albicans mutants affected in multidrug resistance. Infect Immun 63, 4528-4530.
2. Boeke, J. D., Lacroute, F. & Fink, G. R. (1984). A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5 fluoro-orotic acid resistance. Mol Gen Genet 197, 345-346.
3. Broedel, S. E., Jr, Zhao, X.-J. & Cihlar, R. L. (1996). Fatty acid EL44 synthase as a target in the development of new antifungals. Recent Res Dev Antimicrob Agents Chemother 1, 25-33.
4. Bulawa, C. E., Miller, D. W., Henry, L. K. & Becker, J. M. (1995). Attenuated virulence of chitin-deficient mutants of Candida albicans. Proc Natl Acad Sci USA 92, 10570-10574.
5. Cole, M. F., Bowen, W. H., Zhao, X.-J. & Cihlar, R. L. (1995). Avirulence of Candida albicans auxotrophic mutants in a rat model of oropharyngeal candidiasis. FEMS Microbiol Lett 126, 177-180.
6. Cutler, J. E. (1991). Putative virulence factors of Candida albicans. Annu Rev Microbiol 45, 187-218.
7. De Bernardis, F., Adriani, D., Lorenzini, R., Pontieu, E., Carruba, G. & Cassone, A. (1993). Filamentous growth and elevated vaginopathic potential of a nongerminative variant of Candida albicans expressing low virulence in systemic infection. Infect Immun 61, 1500-1508.
8. Fonzi, W. & Irwin, M. Y. (1993). Isogenic strain construction and gene mapping in Candida albicans. Genetics 134, 717-728.
9. Ghannoum, M. A., Spellberg, B., Saporito-Irwin, S. M. & Fonzi, W. F. (1995). Reduced virulence of Candida albicans PHR1 mutants. Infect Immun 63, 4528-4530.
10. Gillum, A. M., Tsay, W. Y. H. & Kirsch, D. R. (1984). Isolation of the Candida albicans gene for orotidine-5′-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations. Mol Gen Genet 198, 179-182.
11. Holmberg, K. & Meyer, R. D. (1986). Fungal infections in patients with AIDS and AIDS-related complex. Scand J infect Dis 18, 179-185.
12. Kuziora, M. A., Chalmer, J. H., Jr, Douglas, M. G., Hitzeman, R. A., Mattick, J. S. & Wakil, S. J. (1983). Molecular cloning of fatty acid synthetase genes from Saccharomyces cerevisiae. J Biol Chem 258, 11648-11653.
13. Lynen, F. (1969). Yeast fatty acid synthase. Methods Enzymol 14, 17-33.
14. McElhaney-Feser, G. E. & Cihlar, R. L. (1994). Purification and characterization of fatty acid synthase from Candida albicans, strain 4918, and two derived spontaneous cerulenin-resistant mutants. J Med Vet Mycol 32, 13-20.
15. Meitner, S. W., Bowen, W. H. & Haidaris, C. G. (1990). Oral and esophageal Candida albicans infection in hyposalivatory rats. Infect Immun 58, 2228-2236.
16. Odds, F. C. (1988). Candida and Candidosis. London: Baillière Tindall.
17. Ōmura, S. (1981). Cerulenin. Methods Enzymol 72, 520-532.
18. Ruchel, R., De Bernardis, F., Ray, T. L., Sullivan, P. A. & Cole, G. T. (1992). Candida acid proteinases. J Med Vet Mycol 29 (Suppl.), 1-9.
19. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
20. Schweizer, E., Müller, G., Roberts, L. M., Schweizer, M., Rösch, J., Weisner, P., Beck, J., Stratman, D. & Zauner, I. (1987). Genetic control of fatty acid synthetase biosynthesis and structure in lower fungi. Fat Sci Technol 89, 570-577.
21. Sherman, F., Fink, G. R. & Hicks, J. B. (1986). Laboratory Course Manual for Methods in Yeast Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
22. Siggaard-Anderson, M. (1993). Conserved residues in condensing enzyme domains of fatty acid synthases and related sequences. Protein Seq Data Anal 5, 325-335.
23. Smith, S. (1994). The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. FASEB J 8, 1248-1259.
24. Soll, D. R. (1992). High frequency switching in Candida albicans. Clin Microbiol Rev 5, 183-203.
25. Stoops, J. K., Awad, E. S., Arslanian, M. J., Gussberg, S. & Wakil, S. J. (1978). Studies on the yeast fatty acid synthetase: subunit composition and structural organization of a large multifunctional enzyme complex. J Biol Chem 253, 4464-4475.
26. Southard, S. B. & Cihlar, R. L. (1995). Analysis and expression of the Candida albicans FAS2 gene. Gene 156, 133-138.
27. Witkowski, A., Rangan, V. S., Randhawa, Z. I., Amy, C. M. & Smith, S. (1991). Structural organization of the multifunctional animal fatty-acid synthase. Eur J Biochem 198, 571-579.