Biotechnological potential of microbial α-galactosidases

Critical Reviews in Biotechnology

Volumn 34, Issue 4, 2014, Pages 307-317

  Katrolia, Priti ^a   Rajashekhara, Eranna ^b   Yan, Qiaojuan ^a   Jiang, Zhengqiang ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b UNIVERSITY OF HORTICULTURAL SCIENCES   (India)

Author keywords

Applications; Biochemical property; Gene cloning; Microorganism; Production; galactosidase

Indexed keywords

APPLICATIONS; CLONE CELLS; CLONING; ENZYMES; GENES; MEDICAL APPLICATIONS; MICROORGANISMS; PAPER AND PULP MILLS; PRODUCTION;

ALPHA-GALACTOSIDASE; BIOCHEMICAL PROPERTIES; BIOTECHNOLOGICAL POTENTIALS; GALACTOSE RESIDUES; GALACTOSIDASES; GENE CLONING; HETEROLOGOUS EXPRESSION; MICROBIAL SOURCES;

SUGAR INDUSTRY;

ALPHA GALACTOSE OLIGOSACCHARIDE; ALPHA GALACTOSIDASE; GALACTOSE OLIGOSACCHARIDE; OLIGOSACCHARIDE; PROTEINASE; RAFFINOSE; RAFFINOSE FAMILY OLIGOSACCHARIDE; REAGENT; SUCROSE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; FUNGAL PROTEIN; RECOMBINANT PROTEIN;

BACTERIUM ISOLATION; BEET SUGAR INDUSTRY; BIOCHEMICAL COMPOSITION; BIOTECHNOLOGICAL PROCEDURES; BIOTECHNOLOGICAL PRODUCTION; CATALYSIS; CLINICAL TRIAL (TOPIC); ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME MECHANISM; ENZYME REPLACEMENT; ENZYME SPECIFICITY; FABRY DISEASE; FEED INDUSTRY; FOOD BIOTECHNOLOGY; FOOD INDUSTRY; GLYCOSYLATION; HETEROLOGOUS EXPRESSION; HUMAN; LEGUME; MICROBIOLOGY; MOLECULAR CLONING; NONHUMAN; PH; PROTEIN EXPRESSION; PROTEIN HYDROLYSIS; PROTEIN STRUCTURE; REVIEW; SOYBEAN MILK; TEMPERATURE; BIOREACTOR; BIOTECHNOLOGY;

ALPHA-GALACTOSIDASE; BACTERIAL PROTEINS; BIOREACTORS; BIOTECHNOLOGY; FUNGAL PROTEINS; RECOMBINANT PROTEINS;

EID: 84911411991     PISSN: 07388551     EISSN: 15497801     Source Type: Journal    
DOI: 10.3109/07388551.2013.794124     Document Type: Review

References (132)

1. Ademark P, de Vries RP, Haegglund P, et al. (2001). Cloning and characterization of Aspergillus niger genes encoding an a-galactosidase and a b-mannosidase involved in galactomannan degradation. Eur J Biochem, 268, 2982-90.
2. Aguilera M, Rakotoarivonina H, Brutus A, et al. (2012). Aga1, the first a-galactosidase from the human bacteria Ruminococcus gnavus E1, efficiently transcribed in gut conditions. Res Microbiol, 163, 14-21.
3. Aleksieva P, Tchorbanov B, Nacheva L. (2010). High-yield production of a-galactosidase excreted from Penicillium chrysogenum and Aspergillus niger. Biotechnol Biotechnol Eq, 24, 1620-3.
4. Anggraeni AA, Sakka M, Kimura T, et al. (2008). Characterization of Bacillus halodurans a-galactosidase Mel4A encoded by the mel4A gene (BH2228). Biosci Biotechnol Biochem, 72, 2459-62.
5. Anisha GS, Prema P. (2008). Cell immobilization technique for the enhanced production of a-galactosidase by Streptomyces griseo-loalbus. Bioresour Technol, 99, 3325-30.
6. Anisha GS, John RP, Prema P. (2009). Biochemical and hydro-lytic properties of multiple thermostable a-galactosidases from Streptomyces griseoloalbus: obvious existence of a novel galactose-tolerant enzyme. Process Biochem, 44, 327-33.
7. Awan MS, Jalal F, Ayub N, et al. (2009). Production and characterization of a-galactosidase by a multiple mutant of Aspergillus niger in solidstate fermentation. Food Technol Biotechnol, 47, 370-80.
8. Baik SH, Saito K, Yokota A, et al. (2000). Molecular cloning and highlevel expression in Escherichia coli of fungal a-galactosidase from Absidia corymbifera IFO8084. J Biosci Bioeng, 90, 168-73.
9. Bakunina IY, Sova VV, Nedashkovskaya OI, et al. (1998). Alpha-galactosidase of the marine bacterium Pseudoalteromonas sp. KMM 701. Biochemistry (Mosc), 63, 1209-15.
10. Balabanova LA, Bakunina IY, Nedashkovskaya OI, et al. (2010). Molecular characterization and therapeutic potential of a marine bacterium Pseudoalteromonas sp. KMM 701 a-galactosidase. Mar Biotechnol (NY), 12, 111-20.
11. Balabanova LA, Bakunina IY, Likhatskaya GN, et al. (2011). Glycoside hydrolases of marine bacteria are promising tools in haemotherapy. The Second International Conference on Bioenvironment, Biodiversity and Renewable Energies. Bionature, Venice, 2011, 47-50.
12. Brouns SJ, Smits N, Wu H, et al. (2006). Identification of a novel a-galactosidase from the hyperthermophilic archaeon Sulfolobus solfataricus. J Bacteriol, 88, 2392-9.
13. Bruel L, Sulzenbacher G, Cervera Tison M, et al. (2011). a-Galactosidase/sucrose kinase (AgaSK), a novel bifunctional enzyme from the human microbiome coupling galactosidase and kinase activities. J Biol Chem, 286, 40814-23.
14. Bulpin PV, Gildley MJ, Jeffcoat R, Underwood DR. (1990). Development of a biotechnological process for the modification of galactomannan polymers with plant a-galactosidase. Carbohydr Polym, 12, 155-68.
15. Cantarel BL, Coutinho PM, Rancurel C, et al. (2009). The Carbohydrate-Active EnZymes database (CAZy): an expert resource for glycoge-nomics. Nucleic Acids Res, 37, D233-8.
16. Cao Y, Huang H, Meng K, et al. (2008). Cloning and functional expression of an a-galactosidase from Yersinia pestis biovar Microtus str. 91001. Biosci Biotechnol Biochem, 72, 2203-5.
17. Cao Y, Wang Y, Meng K, et al. (2009a). A novel protease-resistant a-galactosidase with high hydrolytic activity from Gibberella sp. F75: gene cloning, expression, and enzymatic characterization. Appl Microbiol Biotechnol, 83, 875-84.
18. Cao Y, Wang Y, Luo H, et al. (2009b). Molecular cloning and expression of a novel protease-resistant GH-36 a-galactosidase from Rhizopus sp. F78 ACCC 30795. J Microbiol Biotechnol, 19, 1295-300.
19. Cao Y, Yuan T, Shi P, et al. (2010). Properties of a novel a-galactosidase from Streptomyces sp. S27 and its potential for soybean processing. Enzyme Microb Technol, 47, 305-12.
20. Carrera-Silva EA, Silvestroni A, LeBlanc JG, et al. (2006). A thermostable a-galactosidase from Lactobacillus fermentum CRL722: genetic characterization and main properties. Curr Microbiol, 53, 374-8.
21. Chen Y, Jin M, Esborge T, et al. (2000). Expression and characterization of glycosylated and catalytically active recombinant human a-galactosidase a produced in Pichia pastoris. Protein Expr Purif, 20, 472-84.
22. Chen M, Mustapha A. (2012). Survival of freeze-dried microcapsules of a-galactosidase producing probiotics in a soybar matrix. Food Microbiol, 30, 68-73.
23. Chern MK, Li HY, Chen PF, Chien SF. (2012). Taro a-galactosidase: a new gene product for blood conversion. Biocatal Agric Biotechnol, 1, 135-9.
24. Chiba Y, Sakuraba H, Kotani M, et al. (2002). Production in yeast of a-galactosidase A, a lysosomal enzyme applicable to enzyme replacement therapy for Fabry disease. Glycobiology, 12, 821-8.
25. Clarke JH, Davidson K, Rixon JE, et al. (2000). A comparison of enzyme-aided bleaching of softwood paper pulp using combinations of xylanase, mannanase and a-galactosidase. Appl Microbiol Biotechnol, 53, 661-7.
26. Comfort DA, Bobrov KS, Ivanen DR, et al. (2007). Biochemical analysis of Thermotoga maritima GH36 a-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry, 46, 3319-30.
27. Den Herder IF, Rosell AM, van Zuilen CM, et al. (1992). Cloning and expression of a member of the Aspergillus niger family encoding a-galactosidase. Mol Gen Genet, 233, 404-10.
28. de Vries RP, van den Broeck HC, Dekkers E, et al. (1999). Differential expression of three a-galactosidase genes and a single b-galactosidase gene from Aspergillus niger. Appl Environ Microbiol, 65, 2453-60.
29. Dey PM. (1984). Characteristic features of an a-galactosidase from mung beans. Eur J Biochem, 140, 385-90.
30. Dey PM, Pridham JB. (1972). Biochemistry of a-galactosidases. Adv Enzymol Relat Areas Mol Biol, 36, 91-103.
31. Dion M, Osanjo G, André C, et al. (2001). Identification by saturation mutagenesis of a single residue involved in the a-galactosidase AgaB regioselectivity. Glycoconj J, 18, 457-64.
32. Donkor ON, Henriksson A, Vasiljevic T, Shah NP. (2007). Galactosidase and proteolytic activities of selected probiotic and dairy cultures in fermented soymilk. Food Chem, 104, 10-20.
33. Duffaud GD, McCutchen CM, Leduc P, et al. (1997). Purification and characterization of extremely thermostable b-mannanase, b-mannosidase, and a-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068. Appl Environ Microbiol, 63, 169-77.
34. El-Gindy AA, Ali UF, Ibrahim ZM, Isaac GS. (2008). A cost-effective medium for enhanced production of extracellular a-galactosidase in solid substrate cultures of Aspergillus awamori and A. carbonarius. Aust J Basic Appl Sci, 2, 880-99.
35. Eng CM, Banikazemi M, Gordon RE, et al. (2001). A phase 1/2 clinical trial of enzyme replacement in Fabry disease: pharmacokinetic, substrate clearance, and safety studies. Am J Hum Genet, 68, 711-22.
36. Fallahpour M, Moazami N, Mirdamadi S, et al. (2002). Kinetic behaviour of a-galactosidase produced by Absidia griseola: a comparison between free and immobilized forms of the enzyme. World J Microbiol Biotechnol, 18, 649-53.
37. Farzadi M, Khatami S, Mousavi M, Amirmozafari N. (2011). Purification and characterization of a-galactosidase from Lactobacillus acidophilus. Afr J Biotechnol, 10, 1873-9.
38. Ferreira JG, Reis AP, Guimaraes VM, et al. (2011). Purification and characterization of Aspergillus terreus a-galactosidases and their use for hydrolysis of soymilk oligosaccharides. Appl Biochem Biotechnol, 164, 1111-25.
39. Fredslund F, Hachem MA, Larsen RJ, et al. (2011). Crystal structure of a-galactosidase from Lactobacillus acidophilus NCFM: insight into tetramer formation and substrate binding. J Mol Biol, 412, 466-80.
40. Fridjonsson O, Watzlawick H, Gehweiler A, et al. (1999). Cloning of the gene encoding a novel thermostable a-galactosidase from Thermus brockianus ITI360. Appl Environ Microbiol, 65, 3955-63.
41. Fujimoto Z, Kaneko S, Kim WD, et al. (2009). The tetramer structure of the glycoside hydrolase family 27 a-galactosidase I from Umbelopsis vinacea. Biosci Biotechnol Biochem, 73, 2360-4.
42. Garro MS, Font de Valdez G, Savoy de Giori G. (2004). Temperature effect on activity of Bifidobacterium longum CRL 849 and Lactobacillus fermentum CRL 251 in pure and mixed cultures grown in soymilk. Food Microbiol, 21, 511-18.
43. Ghazi S, Rooke JA, Galbraith H. (2003). Improvement of the nutritive value of soybean meal by protease and a-galactosidase treatment in broiler cockerels and broiler chicks. Br Poult Sci, 44, 410-18.
44. Girigowda K, Kapnoor SS, Kulkarni D, Mulimani VH. (2007). Degradation of raffinose oligosaccharides in soymilk by immobilized a-galactosidase of Aspergillus oryzae. J Microbiol Biotechnol, 17, 1430-6.
45. Golubev AM, Nagem RA, Brandão Neto JR, et al. (2004). Crystal structure of a-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism. J Mol Biol, 339, 413-22.
46. Gote MM, Khan MI, Gokhale DV, et al. (2006). Purification, characterization and substrate specificity of thermostable a-galactosidase from Bacillus stearothermophilus (NCIM-5146). Process Biochem, 41, 1311-17.
47. Goulas T, Goulas A, Tzortzis G, Gibson GR. (2009). A novel a-galactosidase from Bifidobacterium bifidum with transgalactosylat-ing properties: gene molecular cloning and heterologous expression. Appl Microbiol Biotechnol, 82, 471-7.
48. Gurkok S, Cekmecelioglu D, Ogel ZB. (2011). Optimization of culture conditions from Aspergillus sojae expressing an Aspergillus fumigatus a-galactosidase. Bioresour Technol, 102, 4925-9.
49. Hashimoto H, Katayama C, Goto M, Kitahata S. (1993). Purification and some properties of a-galactosidase from Candida guilliermondii H-404. Biosci Biotechnol Biochem, 57, 372-8.
50. Henrissat B. (1991). A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J, 280, 309-16.
51. Henrissat B, Bairoch A. (1993). New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J, 293, 781-8.
52. Henrissat B, Romeu A. (1995). Families, superfamilies, and subfamilies of glycosyl hydrolases. Biochem J, 311, 350-1.
53. Hinz SW, Doeswijk-Voragen CH, Schipperus R, et al. (2006). Increasing the transglycosylation activity of a-galactosidase from Bifidobacterium adolscentis DSM 20083 by site-directed mutagen-esis. Biotechnol Bioeng, 93, 122-31.
54. Ishiguro M, Kaneko S, Kuno A, et al. (2001). Purification and characterization of the recombinant Thermus sp. strain T2 a-galactosidase expressed in Escherichia coli. Appl Environ Microbiol, 67, 1601-16.
55. Janika S, Gernig A, Murray P, et al. (2010). Cloning and expression of a thermostable a-galactosidase from the thermophilic fungus Talaromyces emersonii in the methylotrophic yeast Pichia pastoris. J Microbiol Biotechnol, 20, 1653-63.
56. Jindou S, Karita S, Fujino E, et al. (2002). a-Galactosidase Aga27A, an enzymatic component of the Clostridium josui cellulosome. J Bacteriol, 184, 600-4.
57. Kapnoor S, Mulimani VH. (2010). Production of a-galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk galactooligosaccharide hydrolysis. Braz Arch Biol Technol, 53, 211-18.
58. Katrolia P, Jia H, Yan Q, et al. (2012). Characterization of a protease-resistant a-galactosidase from the thermophilic fungus Rhizomucor miehei and its application in removal of raffinose family oligosaccharides. Bioresour Technol, 110, 578-86.
59. King MR, Yernool DA, Eveleigh DE, Chassy BM. (1998). Thermostable a-galactosidase from Thermotoga neapolitana: cloning, sequencing and expression. FEMS Microbiol Lett, 163, 37-42.
60. Kondoh K, Morisaki K, Kim WD, et al. (2005). Cloning and expression of the gene encoding Streptomyces coelicolor A3(2) a-galactosidase belonging to family 36. Biotechnol Lett, 27, 641-7.
61. Kotwal SM, Gote MM, Khan MI, Khire JM. (1999). Production, purification and characterization of a constitutive intracellular a-galactosidase from the thermophilic fungus Humicola sp. J Ind Microbiol Biotechnol, 23, 661-7.
62. Kulik N, Weignerová L, Filipi T, et al. (2010). The a-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional a-N-acetylgalactosaminidase. Glycobiology, 20, 1410-19.
63. Kumar S, Mishra SK. (2010). Purification and characterization of a thermostable a-galactosidase with transglycosylation activity from Aspergillus parastiticus MTCC-2796. Process Biochem, 45, 1088-93.
64. Kumar SKP, Mulimani VH. (2010). Continuous hydrolysis of raffinose family oligosaccharides in soymilk by fluidized bed reactor. LWT-Food Sci Technol, 43, 220-5.
65. LeBlanc JG, Piard JC, Sesma F, Giori GS. (2005). Lactobacillus fermentum CRL 722 is able to deliver active a-galactosidase activity in the small intestine of rats. FEMS Microbiol Lett, 248, 177-82.
66. Lee J, Park I, Cho J. (2012). Production and partial characterization of a-galactosidase activity from an Antarctic bacterial isolate, Bacillus sp. LX-1. Afr J Biotechnol, 11, 12396-405.
67. Leiro RF, Rodriguez AP, Cerdan ME, et al. (2010). Structural analysis of Saccharomyces cerevisiae a-galactosidase and its complexes with natural substrates reveals new insights into substrate specificity of GH27 glycosidases. J Biol Chem, 285, 28020-33.
68. Liebl W, Wagner B, Schellhase J. (1998). Properties of an a-galacto-sidase, and structure of its gene GalA, within an a-and b-galactosidase utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima. Syst Appl Microbiol, 21, 1-11.
69. Lieshout V, Verhees CH, Oost VD, et al. (2003). Identification and molecular characterization of a novel type of a-galactosidase from Pyrococcus furiosus. Biocatal Biotransform, 21, 243-52.
70. Liu CQ, He GQ. (2012). Multiple a-galactosidases from Aspergillus foetidus ZU-G1: purification, characterization and application in soybean milk hydrolysis. Eur Food Res Technol, 234, 743-51.
71. Liu QP, Sulzenbacher G, Yuan H, et al. (2007a). Bacterial glycosidases for the production of universal red blood cells. Nat Biotechnol, 25, 454-64.
72. Liu C, Ruan H, Shen H, et al. (2007b). Optimization of the fermentation medium for a-galactosidase production from Aspergillus foetidus ZU-G1 using response surface methodology. J Food Sci, 72, M120-5.
73. Liu QP, Yuan H, Bennett EP, et al. (2008). Identification of a GH110 subfamily of a1, 3-galactosidases. J Biol Chem, 283, 8545-54.
74. Liu X, Meng K, Wang Y, et al. (2009). Gene cloning, expression and characterization of an a-galactosidase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive. World J Microbiol Biotechnol, 25, 1633-42.
75. Liu Y, Sun Y, Li Y, et al. (2011). Preparation and characterization of a-galactosidase-loaded chitosan nanoparticles for use in foods. Carbohydr Polym, 83, 1162-8.
76. Luonteri E, Tenkanen M, Viikari L. (1998). Substrate specificities of Penicillium simplicissimum a-galactosidases. Enzyme Microb Technol, 22, 192-8.
77. Manzanares P, de Graaff LH, Visser J. (1998). Characterization of galactosidases from Aspergillus niger: purification of a novel a-galactosidase activity. Enzyme Microb Technol, 22, 383-90.
78. Margolles-Clark E, Tenkanen M, Luonteri E, Penttila M. (1996). Three a-galactosidase genes of Trichoderma reesei cloned by expression in yeast. Eur J Biochem, 240, 104-11.
79. Merceron R, Foucault M, Haser R, et al. (2012). The molecular mechanism of thermostable a-galactosidases AgaA and AgaB, explained by X-ray crystallography and mutational studies. J Biol Chem, 287, 39642-52.
80. Mi S, Meng K, Wang Y, et al. (2007). Molecular cloning and characterization of a novel a-galactosidase gene from Penicillium sp. F63 CGMCC 1669 and expression in Pichia pastoris. Enzyme Microb Technol, 40, 1373-80.
81. Morel CF, Clarke JT. (2009). The use of agalsidase alfa enzyme replacement therapy in the treatment of Fabry disease. Expert Opin Biol Ther, 9, 631-9.
82. Murphy RA, Power RFG. (2002). Expression of an a-galactosidase from Saccharomyces cerevisiae in Aspergillus awamori and Aspergillus oryzae. J Ind Microbiol Biotechnol, 28, 97-102.
83. Naganagouda K, Mulimani VH. (2006). Gelatin blends with alginate: gel fibers for a-galactosidase immobilization and its application in reduction of non-digestible oligosaccharides in soy milk. Process Biochem, 41, 1903-7.
84. Naganagouda K, Mulimani VH. (2008). Aqueous two-phase extraction (ATPE): an attractive and economically viable technology for downstream processing of Aspergillus oryzae a-galactosidase. Process Biochem, 43, 1293-9.
85. Naganagouda K, Prashanth SJ, Shankar SK, et al. (2007). Immobilization of Aspergillus oryzae a-galactosidase in gelatin and its application in removal of flatulence-inducing sugars in soymilk. World J Microbiol Biotechnol, 23, 1131-7.
86. Nakai H, Baumann M, Petersen BO, et al. (2010). Aspergillus nidulans a-galactosidase of glycoside hydrolase family 36 catalyses the formation of a-galacto-oligosaccharides by transglycosylation. FEBS J, 277, 3538-51.
87. Naumoff DG. (2004). Phylogenetic analysis of a-galactosidases of the GH27 family. Mol Biol (Mosk), 38, 388-99.
88. Okuyama M, Kitamura M, Hondoh H, et al. (2009). Catalytic mechanism of retaining a-galactosidase belonging to glycoside hydrolase family 97. J Mol Biol, 392, 1232-41.
89. Olsson ML, Clausen H. (2008). Modifying the red cell surface: towards an ABO-universal blood supply. Br J Haematol, 140, 3-12.
90. Opoku AJ, Tao L, Ferretti JJ, Russell RR. (1991). Biochemical and genetic analysis of Streptococcus mutans a-galactosidase. J Gen Microbiol, 137, 757-64.
91. Pan B, Li D, Piao X, et al. (2002). Effect of dietary supplementation with a-galactosidase preparation and stachyose on growth performance, nutrient digestibility and intestinal bacterial populations of piglets. Arch Tierernahr, 56, 327-37.
92. Pastores GM. (2007). Agalsidase (Replagal) in the treatment of Anderson-Fabry disease. Biologics, 1, 291-300.
93. Patil AG, Kote NV, Mulimani V. (2009). Enzymatic removal of flatulence-inducing sugars in chickpea milk using free and polyvinyl alcohol immobilized a-galactosidase from Aspergillus oryzae. J Ind Microbiol Biotechnol, 36, 29-33.
94. Patil AG, Kumar PK, Mulimani VH, et al. (2010). Alpha-Galactosidase from Bacillus megaterium VHM1 and its application in removal of flatulence-causing factors from soymilk. J Microbiol Biotechnol, 20, 1546-54.
95. Pessela BCC, Fernández-Lafuente R, Torres R, et al. (2007). Production of a thermoresistant a-galactosidase from Thermus sp. strain T2 for food processing. Food Biotechnol, 21, 91-103.
96. Prashanth SJ, Mulimani VH. (2005). Soymilk oligosaccharide hydrolysis by Aspergillus oryzae a-galactosidase immobilized in calcium alginate. Process Biochem, 40, 1199-205.
97. Puchart V, Vrsanská M, Bhat MK, Biely P. (2001). Purification and characterization of a-galactosidase from a thermophilic fungus Thermomyces lanuginosus. Biochim Biophys Acta, 1524, 27-37.
98. Puchart V, Biely P. (2005). Glycosylation of internal sugar residues of oligosaccharides catalyzed by a-galactosidase from Aspergillus fumigatus. Biochim Biophys Acta, 1726, 206-16.
99. Pugalenthi M, Siddhuraju P, Vadivel V. (2006). Effect of soaking followed by cooking and the addition of a-galactosidase on oligosac-charides levels in different Canavalia accessions. J Food Compost Anal, 19, 512-17.
100. Ramalingam R, Saraswathy N, Sadasivam S. (2010). Degradation of flatulence-causing oligosaccharides in soymilk by a-galactosidase-a novel thermotolerant from Penicillium purpurogenum. Indian J Biotechnol, 9, 160-5.
101. Ratto M, Siika-aho M, Buchert MJ, et al. (1993). Enzymatic hydrolysis of isolated and fibre-bound galactomannans from pine-wood and pine kraft pulp. Appl Microbiol Biotechnol, 40, 449-54.
102. Rezessy-Szabó JM, Nguyen QD, Hoschke A, et al. (2007). A novel thermostable a-galactosidase from the thermophilic fungus Thermomyces lanuginosus CBS 395.62/b: purification and characterization. Biochim Biophys Acta, 1770, 55-62.
103. Scalabrini P, Rossi M, Spettoli P, Matteuzzi D. (1998). Characterization of Bifidobacterium strains for use in soymilk fermentation. Int J Food Microbiol, 39, 213-19.
104. Shankar SK, Dhananjay SK, Mulimani VH. (2009). Purification and characterization of thermostable a-galactosidase from Aspergillus terreus (GR). Appl Biochem Biotechnol, 152, 275-85.
105. Shibuya H, Kobayashi H, Sato T, et al. (1997). Purification, characterization and cDNA cloning of a novel a-galactosidase from Mortierella vinacea. Biosci Biotechnol Biochem, 61, 592-8.
106. Shibuya H, Nagasaki H, Kaneko S, et al. (1998). Cloning and high-level expression of a-galactosidase cDNA from Penicillium purpurogenum. Appl Environ Microbiol, 64, 4489-94.
107. Silvestroni A, Connes C, Sesma F, et al. (2002). Characterization of the melA locus for a-galactosidase in Lactobacillus plantarum. Appl Environ Microbiol, 68, 5464-71.
108. Simerská P, Monti D, Cechová I, et al. (2007). Induction and characterization of an unusual a-D-galactosidase from Talaromyces flavus. J Biotechnol, 128, 61-71.
109. Song D, Chang SK. (2006). Enzymatic degradation of oligosaccharides in pinto bean flour. J Agric Food Chem, 54, 1296-301.
110. Spangenberg P, André C, Dion M, et al. (2000). Comparative study of new a-galactosidases in transglycosylation reactions. Carbohydr Res, 329, 65-73.
111. Svastits-Dücsö L, Nguyen QD, Lefler DD, Rezessy-Szabo JM. (2009). Effects of galactomannan as carbon source on production of a-galactosidase by Thermomyces lanuginosus: fermentation, purification and partial characterization. Enzyme Microb Technol, 45, 367-71.
112. Thippeswamy S, Mulimani VH. (2002). Enzymic degradation of raffinose family oligosaccharides in soymilk by immobilized a-galactosidase from Gibberella fujikuroi. Process Biochem, 38, 635-40.
113. Tzortzis G, Jay AJ, Baillon ML, et al. (2003). Synthesis of a-galactooligosaccharides with a-galactosidase from Lactobacillus reuteri of canine origin. Appl Microbiol Biotechnol, 63, 286-92.
114. Ulezlo IV, Zaprometova OM. (1982). Microbial a-galactosidases (a review). Appl Biochem Microbiol, 18, 1-12.
115. Utsumia K, Mitsuhashib F, Asahic K. (2005). Enzyme replacement therapy for Fabry disease: morphologic and histochemical changes in the urinary sediments. Clin Chim Acta, 360, 103-7.
116. Van den Broek LAM, Ton J, Verdoes JC, et al. (1999). Synthesis of a-galacto-oligosaccharides by a cloned a-galactosidase from Bifidobacterium adolescentis. Biotechnol Lett, 21, 441-5.
117. van Laere KM, Hartemink R, Beldman G, et al. (1999). Transglycosidase activity of Bifidobacterium adolescentis DSM 20083 a-galactosidase. Appl Microbiol Biotechnol, 52, 681-8.
118. Varbanets DL, Malanchuk VM, Buglova TT, Kuhlmann RA. (2001). Penicillium sp. 23 a-galactosidase: purification and substrate specificity. Carbohydr Polym, 44, 357-63.
119. Veldman A, Veen WAG, Barug D, van Paridon PA. (1993). Effect of a-galactosides and a-galactosidase in feed on ileal piglet digestive physiology. J Anim Physiol Anim Nutr, 69, 57-65.
120. Viana PA, de Renende ST, Marqoues VM, et al. (2006). Extracellular a-galactosidase from Debaromyces hansenii UFV-1 and its use in the hydrolysis of raffinose oligosaccharides. J Agric Food Chem, 54, 2385-91.
121. Viana PA, de Rezende ST, Passos FML, et al. (2009). Debaryomyces hansenii UFV-1 intracellular a-galactosidase characterization and comparative studies with the extracellular enzyme. J Agric Food Chem, 57, 2515-22.
122. Wang CL, Li DF, Lu WQ, et al. (2004). Influence of cultivation conditions on the a-galactosidase biosynthesis from a novel strain of Penicillium sp. in solid state fermentation. Lett Appl Microbiol, 39, 369-75.
123. Wang H, Luo H, Li J, et al. (2010). An a-galactosidase from an acidophilic Bispora sp. MEY-1 strain acts synergistically with b-mannanase. Bioresour Technol, 101, 8376-82.
124. Weignerová L, Huňková Z, Kuzma M, Křen V. (2001). Enzymatic synthesis of three pNP-a-galactobiopyranosides: application of the library of fungal a-galactosidases. J Mol Catal B: Enzym, 11, 219-24.
125. Weignerová L, Simerská P, Křen V. (2009). a-Galactosidases and their applications in biotransformations. Biocatal Biotransform, 27, 79-89.
126. Xiao M, Tanaka K, Qian XM, et al. (2000). High yield production and characterization of a-galactosidase from Bifidobacterium breve grown on raffinose. Biotechnol Lett, 22, 747-51.
127. Yamashita A, Hashimoto H, Fujita K, et al. (2005). Reverse reaction of Aspergillus niger a-galactosidase in a supersaturated substrate solution: production of a-linked galactooligosaccharide (a-GOS). Biosci Biotechnol Biochem, 69, 1381-8.
128. Zechel DL, Withers SG. (2000). Glycosidase mechanisms: anatomy of a finely tuned catalyst. Acc Chem Res, 33, 11-18.
129. Zeilinger S, Kristufek D, Arisan-Atac I, et al. (1993). Conditions of formation, purification, and characterization of an a-galactosidase of Trichoderma reesei RUTC-30. Appl Environ Microbiol, 59, 1347-53.
130. Zhao H, Lu L, Xiao M, et al. (2008). Cloning and characterization of a novel a-galactosidase from Bifidobacterium breve 203 capable of synthesizing Gal-a-1, 4 linkage. FEMS Microbiol Lett, 285, 278-83.
131. Zhou J, Shi P, Huang H, Cao Y, et al. (2010). A new a-galactosidase from symbiotic Flavobacterium sp. TN17 reveals four residues essential for a-galactosidase activity of gastrointestinal bacteria. Appl Microbiol Biotechnol, 88, 1297-309.
132. Zhu A, Leng L, Monahan C, et al. (1996). Characterization of recombinant a-galactosidase for use in seroconversion from blood group B to O of human erythrocytes. Arch Biochem Biophys, 327, 324-9.