Cloning and expression of a thermostable α-galactosidase from the thermophilic fungus Talaromyces emersonii in the methylotrophic yeast Pichia pastoris

Journal of Microbiology and Biotechnology

Volumn 20, Issue 12, 2010, Pages 1653-1663

  Similä, Janika ^a   Gernig, Anita ^b   Murray, Patrick ^c   Fernandes, Sara ^d   Tuohy, Maria G ^a  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^b AUSTRIAN AGENCY FOR HEALTH AND FOOD SAFETY   (Austria)

^c Athletic Rehabilitation Therapy Ireland   (Ireland)

^d UNIVERSITY OF MINHO   (Portugal)

Author keywords

galactosidase; Overexpression; Pichia pastoris; Talaromyces emersonii; Thermostability

Indexed keywords

ALPHA GALACTOSIDASE; AMINO ACID DERIVATIVE; GALACTOSE; GLYCOPROTEIN; HISTIDINE; OLIGOMER; POLYMER; RECOMBINANT ENZYME;

ALPHA GALACTOSIDASE GENE; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BASE PAIRING; ENZYME RELEASE; ENZYME SUBSTRATE; FUNGAL GENE; GENE OVEREXPRESSION; GENETIC STABILITY; INTRON; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PICHIA PASTORIS; PLANT BIOTECHNOLOGY; PROTEIN FAMILY; TALAROMYCES; TALAROMYCES EMERSONII; TEMPERATURE; THERMOSTABILITY;

ALPHA-GALACTOSIDASE; AMINO ACID SEQUENCE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA, FUNGAL; ENZYME STABILITY; GENE EXPRESSION; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; INTRONS; KINETICS; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; PICHIA; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY; TALAROMYCES;

FUNGI; PICHIA PASTORIS; TALAROMYCES EMERSONII;

EID: 78650843968     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1005.05043     Document Type: Article

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