Identification and molecular characterization of a novel type of α-galactosidase from Pyrococcus furiosus

Biocatalysis and Biotransformation

Volumn 21, Issue 4-5, 2003, Pages 243-252

  Van Lieshout, Johan F T ^a   Verhees, Corné H ^a,d   Ettema, Thijs J G ^a   Van Der Sar, Sjaak ^a   Imamura, Hiromi ^b   Matsuzawa, Hiroshi ^c   Van Der Oost, John ^a   De Vos, Willem M ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b UNIVERSITY OF TOKYO   (Japan)

^c AOMORI UNIVERSITY   (Japan)

^d DSM FOOD SPECIALTIES   (Netherlands)

Author keywords

galactosidase; Catalytic residues; Chemical rescue; GH family 57; Glycoside hydrolases; Pyrococcus furiosus; Site directed mutagenesis; Thermostable

Indexed keywords

AMINO ACIDS; CLONING; ENZYMES; ESCHERICHIA COLI; THERMAL EFFECTS;

HOMOLOGY;

BIOCATALYSTS;

4 NITROPHENYLGALACTOSIDE; ALANINE; ALPHA GALACTOSIDASE; AMYLASE; GLUTAMIC ACID; GLYCOSIDASE; MELIBIOSE; RAFFINOSE; RECOMBINANT ENZYME;

AMINO ACID SEQUENCE; ARCHAEBACTERIUM; CATALYSIS; CATALYST; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE IDENTIFICATION; GENETIC CODE; HALF LIFE TIME; HYDROLYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PYROCOCCUS FURIOSUS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; TEMPERATURE DEPENDENCE; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

ARCHAEA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA; PYROCOCCUS; PYROCOCCUS FURIOSUS;

EID: 0347337762     PISSN: 10242422     EISSN: None     Source Type: Journal    
DOI: 10.1080/10242420310001614342     Document Type: Conference Paper

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