Increasing the transglycosylation activity of α-galactosidase from Bifidobacterium adolescentis DSM 20083 by site-directed mutagenesis

Biotechnology and Bioengineering

Volumn 93, Issue 1, 2006, Pages 122-131

  Hinz, Sandra W A ^a   Doeswijk Voragen, Chantal H L ^a   Schipperus, Roelof ^a   Van Den Broek, Lambertus A M ^a   Vincken, Jean Paul ^a   Voragen, Alphons G J ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

galactosidase; Homology modeling; Site directed mutagenesis; Substrate specificity; Thermostability; Transglycosylation

Indexed keywords

AMINO ACIDS; BACTERIA; CATALYST ACTIVITY; CONCENTRATION (PROCESS); MUTAGENESIS; PH EFFECTS; THERMODYNAMIC STABILITY;

Α-GALACTOSIDASE; HOMOLOGY MODELING; SITE-DIRECTED MUTAGENESIS; SUBSTRATE SPECIFICITY; THERMOSTABILITY; TRANSGLYCOSYLATION;

ENZYMES;

ALDITOL; ALPHA GALACTOSIDASE; DISACCHARIDE; HEXOSE; HEXURONIC ACID; MELIBIOSE; PENTOSE;

AMINO ACID SEQUENCE; ARTICLE; BIFIDOBACTERIUM ADOLESCENTIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GLYCOSYLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

ALPHA-GALACTOSIDASE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BIFIDOBACTERIUM; CATALYTIC DOMAIN; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; MELIBIOSE; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN STRUCTURE, TERTIARY; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

BIFIDOBACTERIUM ADOLESCENTIS;

EID: 30944433165     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20713     Document Type: Article

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