Identification by saturation mutagenesis of a single residue involved in the α-galactosidase AgaB regioselectivity

Glycoconjugate Journal

Volumn 18, Issue 6, 2001, Pages 457-464

  Dion, Michel ^a   Osanjo, George ^a   André, Corinne ^a   Spangenberg, Petra ^a   Rabiller, Claude ^a   Tellier, Charles ^a  

^a UNIVERSITÉ DE NANTES   (France)

Author keywords

Directed evolution; Regioselectivity; Saturation mutagenesis; Transglycosylation

Indexed keywords

ALPHA GALACTOSIDASE; ALPHA N ACETYLGALACTOSAMINIDASE;

ARTICLE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME KINETICS; EVOLUTION; GENE MUTATION; GEOBACILLUS STEAROTHERMOPHILUS; GLYCOSYLATION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; STEREOCHEMISTRY; THIN LAYER CHROMATOGRAPHY;

ALPHA-N-ACETYLGALACTOSAMINIDASE; BACILLUS STEAROTHERMOPHILUS; CLONING, MOLECULAR; CODON; DIRECTED MOLECULAR EVOLUTION; DISACCHARIDES; GLYCOSYLATION; HEXOSAMINIDASES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

GEOBACILLUS STEAROTHERMOPHILUS;

EID: 0035744533     PISSN: 02820080     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1016034101436     Document Type: Article

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