Characterization of galactosidases from Aspergillus niger: Purification of a novel α-galactosidase activity

Enzyme and Microbial Technology

Volumn 22, Issue 5, 1998, Pages 383-390

  Manzanares, Paloma ^a   De Graaff, Leo H ^a   Visser, Jaap ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

galactosidase; galactosidase; Aspergillus niger; Purification

Indexed keywords

AMINO ACIDS; CATALYST ACTIVITY; FUNGI; NITROGEN COMPOUNDS; PH EFFECTS; PLANT CELL CULTURE; PURIFICATION; SUBSTRATES; SUGAR BEETS; SUGARS; THERMAL EFFECTS;

GALACTOSIDASE; LACTOSE; NITROPHENYL; PECTIN GALACTAN;

ENZYMES;

4 NITROPHENYLGALACTOSIDE; ALPHA GALACTOSIDASE; BETA GALACTOSIDASE; FUNGAL PROTEIN; GALACTAN; GLUCAN SYNTHASE; LACTOSE; XYLAN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; ASPERGILLUS NIGER; DEGLYCOSYLATION; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SUBSTRATE; MOLECULAR WEIGHT; NONHUMAN; PH; TEMPERATURE;

ALPHA-GALACTOSIDASE; AMINO ACID SEQUENCE; ASPERGILLUS NIGER; ENZYME STABILITY; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; ISOELECTRIC POINT; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SUBSTRATE SPECIFICITY; TEMPERATURE;

ASPERGILLUS NIGER; BETA VULGARIS SUBSP. VULGARIS; CITRUS LIMON; FUNGI; PHASEOLUS (ANGIOSPERM);

EID: 0032055401     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(97)00207-X     Document Type: Article

References (43)

1. Dey P. M., Pridham J. B. Biochemistry of α-galactosidases. Adv. Enzymol. 36:1972;91-130.
2. Shukla T. P. Beta-galactosidase technology A solution to the lactose problem . CRC Crit. Rev. Food Technol. 5:1975;325-356.
3. Mustranta A., Linko M., Harju M., Heikonen M. Production and use of lactase. Kem. Kemi. 6:1979;306-308.
4. Dahlqvist A., Mattiasson B., Mosbach K. Hydrolysis of β-galactosides using polymer-entrapped lactase. A study towards producing lactose-free milk. Biotechnol. Bioeng. 15:1973;395-402.
5. Kumar V., Ramakrishnan S., Teeri T. T., Knowles J. K. C., Hartley B. S. Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grown on whey permeate. Bio/Technology. 10:1992;82-85.
6. Gekas V., Lopez-Leiva M. Hydrolysis of lactose A literature review . Process Biochem. 20:1985;2-12.
7. Kobayashi H., Suzuki H. Studies on the decomposition of raffinose by α-galactosidase of mold. J. Ferment. Technol. 50:1972;625-632.
8. Cristofaro E., Mottu F., Wuhrmann J. J. Involvement of the raffinose family of oligosaccharides in flatulence. Sipple H. L., McNutt K. Sugars in Nutrition. 1974;313-336 Academic Press, New York.
9. Cruz R., Park Y. K. Production of fungal α-galactosidase and its application to hydrolysis of galactooligosaccharides in soybean milk. J. Food Sci. 47:1982;1973-1975.
10. Somiari R. I., Balogh E. Properties of an extracellular glycosidase of Aspergillus niger suitable for removal of oligosaccharides from cowpea meal. Enzyme Microb. Technol. 17:1995;311-316.
11. Dey P. M., Patel S., Brownleader M. D. Induction of α-galactosidase in Penicillium ochrochloron by guar (Cyamopsis tetragonobola) gum. Biotechnol. Appl. Biochem. 17:1993;361-371.
12. Nevalainen K. M. H. Induction, isolation, and characterization of Aspergillus niger mutant strains producing elevated levels of β-galactosidase. Appl. Environ. Microbiol. 41:1981;593-596.
13. Barbesgaard P. Industrial enzymes produced by members of the genus Aspergillus. Smith J. E., Pateman J. A. In Genetics and Physiology of Aspergillus. Br. Myc. Soc. Symp. Series no. 1 . 1977;391-404 Academic Press, London.
14. Bennett J. W. Molds, manufacturing and molecular genetics. Timberlake W. E. Molecular Genetics of Filamentous Fungi. 1985;345-366 Alan R. Liss, New York.
15. Bahl O. P., Agrawal K. M. L. Glycosidases of Aspergillus niger. Purification and characterization of α- and β-galactosidases and β-N-acetylglucosaminidase. J. Biol. Chem. 244:1969;2970-2978.
16. Adya S., Elbein A. D. Glycoprotein enzymes secreted by Aspergillus niger: Purification and properties of α-galactosidase. J. Bacteriol. 129:1977;850-856.
17. Widmer F., Leuba J-L. β-Galactosidase from Aspergillus niger. Separation and characterization of three multiple forms. Eur. J. Biochem. 100:1979;559-567.
18. Greenberg N. A., Mahoney R. R. Rapid purification of β-galactosidase (Aspergillus niger) from a commercial preparation. J. Food Sci. 46:1981;684-687.
19. Mbuyi-Kalala A., Schnek A. G., Léonis J. Separation and characterization of four enzyme forms of β-galactosidase from Saccharomyces lactis. Eur. J. Biochem. 178:1988;437-443.
20. Ross G. S., Wegrzyn T., MacRae E. A., Redgwell R. J. Apple β-galactosidase. Activity against cell wall polysaccharides and characterization of a related cDNA clone. Plant Physiol. 106:1994;521-528.
21. Kaneko R., Kusakabe I., Ida E., Murakami K. Substrate specificity of α-galactosidase from Aspergillus niger 5-16. Agric. Biol. Chem. 55:1991;109-115.
22. den Herder I. F., Mateo Rosell A. M., van Zuilen C. M., Punt P. J., van den Hondel C. A. M. J. J. Cloning and expression of a member of the Aspergillus niger gene family encoding α-galactosidase. Mol. Gen. Genet. 233:1992;404-410.
23. Pontecorvo G., Roper J. A., Hemmons L. J., MacDonald K. D., Bufton A. W. J. The genetics of Aspergillus nidulans. Adv. Genet. 5:1953;141-238.
24. van der Veen P., Flipphi M. J. A., Voragen A. G. J., Visser J. Induction, purification and characterization of arabinases produced by Aspergillus niger. Arch. Microbiol. 157:1991;23-28.
25. Bailey M. J., Biely P., Poutanen K. Interlaboratory testing of methods for assay of xylanase activity. J. Biotechnol. 23:1992;257-270.
26. Biely P., Markovič O., Mislovičová D. Sensitive detection of endo-1,4-β-glucanases and endo-1,4-β-xylanases in gels. Anal. Biochem. 144:1985;147-151.
27. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
28. Zeilinger S., Kristufek D., Arisan-Atac I., Hodits R., Kubicek C. P. Conditions of formation, purification, and characterization of an α-galactosidase of Trichoderma reesei RUT C-30. Appl. Environ. Microbiol. 59:1993;1347-1353.
29. Margolles-Clark E., Tenkanen M., Luonteri E., Penttilä M. Three α-galactosidase genes of Trichoderma reesei cloned by expression in yeast. Eur. J. Biochem. 240:1996;104-111.
30. Civas A., Eberhard R., Le Dizet P., Petek F. Glycosidases induced in Aspergillus tamarii. Mycelial α-D-galactosidases. Biochem. J. 219:1984;849-855.
31. Shibuya H., Kobayashi H., Kasamo K., Kusakabe I. Nucleotide sequence of α-galactosidase cDNA from Mortierella vinacea. Biosci. Biotechnol. Biochem. 59:1995;1345-1348.
32. Gonzalez R. R., Monsan P. Purification and some characteristics of β-galactosidase from Aspergillus fonsecaeus. Enzyme Microb. Technol. 13:1991;349-352.
33. Neustroev K. N., Krylov A. S., Abroskina O. N., Firsov L. M., Nasonov V. V., Khorlin A. Y. Isolation and properties of α-galactosidase from Aspergillus awamori. Biochem. USSR. 56:1991;288-296.
34. Shibuya H., Kobayashi H., Park G. G., Komatsu Y., Sato T., Kaneko R., Nagasaki H., Yoshida S., Kasamo K., Kusakabe I. Purification and some properties of α-galactosidase from Penicillium purpurogenum. Biosci. Biotechnol. Biochem. 59:1995;2333-2335.
35. van Peij N. N. M. E., Brinkmann J., Vršanská M., Visser J., de Graaff L. H. β-Xylosidase activity, encoded by xlnD, is essential for complete hydrolysis of xylan by Aspergillus niger but not for induction of the xylanolytic enzyme spectrum. Eur. J. Biochem. 245:1997;164-173.
36. Himmel M. E., Adney W. S., Fox J. W., Mitchell D. J., Baker J. O. Isolation and characterization of two forms of β-D-glucosidase from Aspergillus niger. Appl. Biochem. Biotechnol. 39/40:1993;213-225.
37. Park Y. K., DeSanti M. S. S., Pastore G. M. Production and characterization of β-galactosidase from Aspergillus oryzae. J. Food Sci. 44:1979;100-103.
38. Cavaille D., Combes D. Characterization of β-galactosidase from Kluyveromyces lactis. Biotechnol. Appl. Biochem. 22:1995;55-64.
39. Golden K. D., John M. A., Kean E. A. β-galactosidase from Coffea arabica and its role in fruit ripening. Phytochemistry. 34:1993;355-360.
40. Annunziato M. E., Mahoney R. R. Partial purification and characterization of α-galactosidase from Aspergillus oryzae. J. Food Biochem. 11:1987;263-277.
41. Zapater I. G., Ullah A. H. J., Wodzinski R. J. Extracellular α-galactosidase (E.C.3.2.1.22) from Aspergillus ficuum NRRL 3135. Purification and characterization. Prep. Biochem. 20:1990;263-296.
42. Civas A., Eberhard R., Le Dizet P., Petek F. Glycosidases induced in Aspergillus tamarii. Secreted α-D-galactosidase and β-D-mannanase. Biochem. J. 219:1984;857-863.
43. Sekimata M., Ogura K., Tsumuraya Y., Hashimoto Y., Yamamoto S. A β-galactosidase from radish (Raphanus sativus L.) seeds. Plant Physiol. 90:1989;567-574.