The molecular mechanism of thermostable α-galactosidases AgaA and AgaB explained by X-ray crystallography and mutational studies

Journal of Biological Chemistry

Volumn 287, Issue 47, 2012, Pages 39642-39652

  Merceron, Romain ^a   Foucault, Marine ^a   Haser, Richard ^a   Mattes, Ralf ^b   Watzlawick, Hildegard ^b   Gouet, Patrice ^a  

^a INSTITUT DE BIOLOGIE ET CHIMIE DES PROTÉINES   (France)

^b UNIVERSITY OF STUTTGART   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTALLOGRAPHY;

ACTIVE SITE; CATALYTIC PROPERTIES; GALACTOSIDASES; INDUCED-FIT MECHANISM; MOLECULAR MECHANISM; SEQUENCE IDENTITY; TRANSGLYCOSYLATION;

X RAY CRYSTALLOGRAPHY;

AGAA PROTEIN; AGAB PROTEIN; ALPHA GALACTOSIDASE; ISOENZYME; RAFFINOSE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME LOCALIZATION; ENZYME MODIFICATION; ENZYME SPECIFICITY; GENE MUTATION; GENE SEQUENCE; GLYCOSYLATION; MOLECULAR MECHANICS; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY; AMINO ACID SUBSTITUTION; CHEMISTRY; ENZYMOLOGY; GENETICS; GEOBACILLUS STEAROTHERMOPHILUS; METABOLISM; MISSENSE MUTATION; PROTEIN TERTIARY STRUCTURE; STRUCTURAL HOMOLOGY; STRUCTURE ACTIVITY RELATION;

ALPHA-GALACTOSIDASE; AMINO ACID SUBSTITUTION; CRYSTALLOGRAPHY, X-RAY; GEOBACILLUS STEAROTHERMOPHILUS; MUTATION, MISSENSE; PROTEIN STRUCTURE, TERTIARY; RAFFINOSE; STRUCTURAL HOMOLOGY, PROTEIN; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84873971576     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.394114     Document Type: Article

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