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Volumn 24, Issue 6, 2013, Pages 1108-1115

Metabolic flux rewiring in mammalian cell cultures

Author keywords

[No Author keywords available]

Indexed keywords

FEEDING STRATEGIES; FLUX REGULATION; GLUCOSE AND GLUTAMINE METABOLISMS; INTRACELLULAR CARBON; MAMMALIAN CELL CULTURE; METABOLIC PHENOTYPE; NOVEL STRATEGIES; OXIDATIVE METABOLISM;

EID: 84887619565     PISSN: 09581669     EISSN: 18790429     Source Type: Journal    
DOI: 10.1016/j.copbio.2013.04.016     Document Type: Review
Times cited : (101)

References (69)
  • 1
    • 66249108601 scopus 로고    scopus 로고
    • Understanding the Warburg effect: the metabolic requirements of cell proliferation
    • Vander Heiden M.G., Cantley L.C., Thompson C.B. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science 2009, 324:1029-1033.
    • (2009) Science , vol.324 , pp. 1029-1033
    • Vander Heiden, M.G.1    Cantley, L.C.2    Thompson, C.B.3
  • 2
    • 33749256407 scopus 로고    scopus 로고
    • Engineering cells for cell culture bioprocessing-physiological fundamentals
    • Seth G., Hossler P., Yee J.C., Hu W.S. Engineering cells for cell culture bioprocessing-physiological fundamentals. Adv Biochem Eng Biotechnol 2006, 101:119-164.
    • (2006) Adv Biochem Eng Biotechnol , vol.101 , pp. 119-164
    • Seth, G.1    Hossler, P.2    Yee, J.C.3    Hu, W.S.4
  • 3
    • 37449034854 scopus 로고    scopus 로고
    • Beyond aerobic glycolysis: transformed cells can engage in glutamine metabolism that exceeds the requirement for protein and nucleotide synthesis
    • DeBerardinis R.J., Mancuso A., Daikhin E., Nissim I., Yudkoff M., Wehrli S., Thompson C.B. Beyond aerobic glycolysis: transformed cells can engage in glutamine metabolism that exceeds the requirement for protein and nucleotide synthesis. Proc Natl Acad Sci U S A 2007, 104:19345-19350.
    • (2007) Proc Natl Acad Sci U S A , vol.104 , pp. 19345-19350
    • DeBerardinis, R.J.1    Mancuso, A.2    Daikhin, E.3    Nissim, I.4    Yudkoff, M.5    Wehrli, S.6    Thompson, C.B.7
  • 4
    • 37449024702 scopus 로고    scopus 로고
    • The biology of cancer: metabolic reprogramming fuels cell growth and proliferation
    • DeBerardinis R.J., Lum J.J., Hatzivassiliou G., Thompson C.B. The biology of cancer: metabolic reprogramming fuels cell growth and proliferation. Cell Metab 2008, 7:11-20.
    • (2008) Cell Metab , vol.7 , pp. 11-20
    • DeBerardinis, R.J.1    Lum, J.J.2    Hatzivassiliou, G.3    Thompson, C.B.4
  • 5
    • 84868481008 scopus 로고    scopus 로고
    • Multivariate analysis of cell culture bioprocess data-lactate consumption as process indicator
    • Le H., Kabbur S., Pollastrini L., Sun Z., Mills K., Johnson K., Karypis G., Hu W.S. Multivariate analysis of cell culture bioprocess data-lactate consumption as process indicator. J Biotechnol 2012, 162:210-223.
    • (2012) J Biotechnol , vol.162 , pp. 210-223
    • Le, H.1    Kabbur, S.2    Pollastrini, L.3    Sun, Z.4    Mills, K.5    Johnson, K.6    Karypis, G.7    Hu, W.S.8
  • 6
    • 84858000888 scopus 로고    scopus 로고
    • On metabolic shift to lactate consumption in fed-batch culture of mammalian cells
    • Mulukutla B.C., Gramer M., Hu W.S. On metabolic shift to lactate consumption in fed-batch culture of mammalian cells. Metab Eng 2012, 14:138-149.
    • (2012) Metab Eng , vol.14 , pp. 138-149
    • Mulukutla, B.C.1    Gramer, M.2    Hu, W.S.3
  • 7
    • 8144228566 scopus 로고    scopus 로고
    • Why do cancers have high aerobic glycolysis?
    • Gatenby R.A., Gillies R.J. Why do cancers have high aerobic glycolysis?. Nat Rev Cancer 2004, 4:891-899.
    • (2004) Nat Rev Cancer , vol.4 , pp. 891-899
    • Gatenby, R.A.1    Gillies, R.J.2
  • 9
    • 44349186331 scopus 로고    scopus 로고
    • Influence of genotype and nutrition on survival and metabolism of starving yeast
    • Boer V.M., Amini S., Botstein D. Influence of genotype and nutrition on survival and metabolism of starving yeast. Proc Natl Acad Sci U S A 2008, 105:6930-6935.
    • (2008) Proc Natl Acad Sci U S A , vol.105 , pp. 6930-6935
    • Boer, V.M.1    Amini, S.2    Botstein, D.3
  • 10
    • 61849135453 scopus 로고    scopus 로고
    • Tumor suppressors and cell metabolism: a recipe for cancer growth
    • Jones R.G., Thompson C.B. Tumor suppressors and cell metabolism: a recipe for cancer growth. Genes Dev 2009, 23:537-548.
    • (2009) Genes Dev , vol.23 , pp. 537-548
    • Jones, R.G.1    Thompson, C.B.2
  • 11
    • 51049091904 scopus 로고    scopus 로고
    • Quantifying reductive carboxylation flux of glutamine to lipid in a brown adipocyte cell line
    • Yoo H., Antoniewicz M.R., Stephanopoulos G., Kelleher J.K. Quantifying reductive carboxylation flux of glutamine to lipid in a brown adipocyte cell line. J Biol Chem 2008, 283:20621-20627.
    • (2008) J Biol Chem , vol.283 , pp. 20621-20627
    • Yoo, H.1    Antoniewicz, M.R.2    Stephanopoulos, G.3    Kelleher, J.K.4
  • 13
    • 77955843732 scopus 로고    scopus 로고
    • Glucose metabolism in mammalian cell culture: new insights for tweaking vintage pathways
    • Mulukutla B.C., Khan S., Lange A., Hu W.S. Glucose metabolism in mammalian cell culture: new insights for tweaking vintage pathways. Trends Biotechnol 2010, 28:476-484.
    • (2010) Trends Biotechnol , vol.28 , pp. 476-484
    • Mulukutla, B.C.1    Khan, S.2    Lange, A.3    Hu, W.S.4
  • 14
    • 12944262229 scopus 로고    scopus 로고
    • Molecular and cellular regulation of glucose transporter (GLUT) proteins in cancer
    • Macheda M.L., Rogers S., Best J.D. Molecular and cellular regulation of glucose transporter (GLUT) proteins in cancer. J Cell Physiol 2005, 202:654-662.
    • (2005) J Cell Physiol , vol.202 , pp. 654-662
    • Macheda, M.L.1    Rogers, S.2    Best, J.D.3
  • 15
    • 0033079597 scopus 로고    scopus 로고
    • Oncogenic alterations of metabolism
    • Dang C.V., Semenza G.L. Oncogenic alterations of metabolism. Trends Biochem Sci 1999, 24:68-72.
    • (1999) Trends Biochem Sci , vol.24 , pp. 68-72
    • Dang, C.V.1    Semenza, G.L.2
  • 16
    • 0030049769 scopus 로고    scopus 로고
    • Comparative analysis of glucose and glutamine metabolism in transformed mammalian cell lines, insect and primary liver cells
    • Neermann J., Wagner R. Comparative analysis of glucose and glutamine metabolism in transformed mammalian cell lines, insect and primary liver cells. J Cell Physiol 1996, 166:152-169.
    • (1996) J Cell Physiol , vol.166 , pp. 152-169
    • Neermann, J.1    Wagner, R.2
  • 17
    • 0027692808 scopus 로고
    • Glucose and glutamine metabolism of a murine B-lymphocyte hybridoma grown in batch culture
    • Fitzpatrick L., Jenkins H.A., Butler M. Glucose and glutamine metabolism of a murine B-lymphocyte hybridoma grown in batch culture. Appl Biochem Biotechnol 1993, 43:93-116.
    • (1993) Appl Biochem Biotechnol , vol.43 , pp. 93-116
    • Fitzpatrick, L.1    Jenkins, H.A.2    Butler, M.3
  • 18
    • 35649014840 scopus 로고    scopus 로고
    • Hypoxia-inducible factor 1 and dysregulated c-Myc cooperatively induce vascular endothelial growth factor and metabolic switches hexokinase 2 and pyruvate dehydrogenase kinase 1
    • Kim J.W., Gao P., Liu Y.C., Semenza G.L., Dang C.V. Hypoxia-inducible factor 1 and dysregulated c-Myc cooperatively induce vascular endothelial growth factor and metabolic switches hexokinase 2 and pyruvate dehydrogenase kinase 1. Mol Cell Biol 2007, 27:7381-7393.
    • (2007) Mol Cell Biol , vol.27 , pp. 7381-7393
    • Kim, J.W.1    Gao, P.2    Liu, Y.C.3    Semenza, G.L.4    Dang, C.V.5
  • 19
    • 27844526600 scopus 로고    scopus 로고
    • Akt-dependent transformation: there is more to growth than just surviving
    • Plas D.R., Thompson C.B. Akt-dependent transformation: there is more to growth than just surviving. Oncogene 2005, 24:7435-7442.
    • (2005) Oncogene , vol.24 , pp. 7435-7442
    • Plas, D.R.1    Thompson, C.B.2
  • 21
    • 17844383964 scopus 로고    scopus 로고
    • Perturbational profiling of a cell-line model of tumorigenesis by using metabolic measurements
    • Ramanathan A., Wang C., Schreiber S.L. Perturbational profiling of a cell-line model of tumorigenesis by using metabolic measurements. Proc Natl Acad Sci U S A 2005, 102:5992-5997.
    • (2005) Proc Natl Acad Sci U S A , vol.102 , pp. 5992-5997
    • Ramanathan, A.1    Wang, C.2    Schreiber, S.L.3
  • 22
    • 33644614520 scopus 로고    scopus 로고
    • HIF-1-mediated expression of pyruvate dehydrogenase kinase: a metabolic switch required for cellular adaptation to hypoxia
    • Kim J.W., Tchernyshyov I., Semenza G.L., Dang C.V. HIF-1-mediated expression of pyruvate dehydrogenase kinase: a metabolic switch required for cellular adaptation to hypoxia. Cell Metab 2006, 3:177-185.
    • (2006) Cell Metab , vol.3 , pp. 177-185
    • Kim, J.W.1    Tchernyshyov, I.2    Semenza, G.L.3    Dang, C.V.4
  • 25
    • 33846061120 scopus 로고    scopus 로고
    • 13C-based flux analysis
    • 13C-based flux analysis. Mol Syst Biol 2006, 2:62.
    • (2006) Mol Syst Biol , vol.2 , pp. 62
    • Sauer, U.1
  • 26
    • 84855596555 scopus 로고    scopus 로고
    • Towards dynamic metabolic flux analysis in CHO cell cultures
    • Ahn W.S., Antoniewicz M.R. Towards dynamic metabolic flux analysis in CHO cell cultures. Biotechnol J 2012, 7:61-74.
    • (2012) Biotechnol J , vol.7 , pp. 61-74
    • Ahn, W.S.1    Antoniewicz, M.R.2
  • 29
    • 84870668228 scopus 로고    scopus 로고
    • 13C]glutamine provide new insights into CHO cell metabolism
    • 13C]glutamine provide new insights into CHO cell metabolism. Metab Eng 2013, 15:34-47.
    • (2013) Metab Eng , vol.15 , pp. 34-47
    • Ahn, W.S.1    Antnoniewicz, M.R.2
  • 30
    • 84878388792 scopus 로고    scopus 로고
    • Peak antibody production is associated with increased oxidative metabolism in an industrially relevant fed-batch CHO cell culture
    • Templeton N., Dean J., Reddy P., Young J.D. Peak antibody production is associated with increased oxidative metabolism in an industrially relevant fed-batch CHO cell culture. Biotechnol Bioeng 2013, 110:2013-2024.
    • (2013) Biotechnol Bioeng , vol.110 , pp. 2013-2024
    • Templeton, N.1    Dean, J.2    Reddy, P.3    Young, J.D.4
  • 32
    • 84872376676 scopus 로고    scopus 로고
    • 13C flux analysis of Myc-induced metabolic reprogramming in B-cells
    • 13C flux analysis of Myc-induced metabolic reprogramming in B-cells. Metab Eng 2013, 15:206-217.
    • (2013) Metab Eng , vol.15 , pp. 206-217
    • Murphy, T.A.1    Dang, C.V.2    Young, J.D.3
  • 33
    • 79955939126 scopus 로고    scopus 로고
    • Unraveling the metabolism of HEK-293 cells using lactate isotopomer analysis
    • Henry O., Jolicoeur M., Kamen A. Unraveling the metabolism of HEK-293 cells using lactate isotopomer analysis. Bioprocess Biosyst Eng 2011, 34:263-273.
    • (2011) Bioprocess Biosyst Eng , vol.34 , pp. 263-273
    • Henry, O.1    Jolicoeur, M.2    Kamen, A.3
  • 34
    • 0030571616 scopus 로고    scopus 로고
    • Energy metabolism and ATP balance in animal cell cultivation using a stoichiometrically based reaction network
    • Xie L., Wang D.I. Energy metabolism and ATP balance in animal cell cultivation using a stoichiometrically based reaction network. Biotechnol Bioeng 1996, 52:591-601.
    • (1996) Biotechnol Bioeng , vol.52 , pp. 591-601
    • Xie, L.1    Wang, D.I.2
  • 35
    • 0028077867 scopus 로고
    • Profile of energy metabolism in a murine hybridoma: glucose and glutamine utilization
    • Petch D., Butler M. Profile of energy metabolism in a murine hybridoma: glucose and glutamine utilization. J Cell Physiol 1994, 161:71-76.
    • (1994) J Cell Physiol , vol.161 , pp. 71-76
    • Petch, D.1    Butler, M.2
  • 37
    • 0035829827 scopus 로고    scopus 로고
    • Decoupling cell growth and product formation in Chinese hamster ovary cells through metabolic control
    • Altamirano C., Cairo J.J., Godia F. Decoupling cell growth and product formation in Chinese hamster ovary cells through metabolic control. Biotechnol Bioeng 2001, 76:351-360.
    • (2001) Biotechnol Bioeng , vol.76 , pp. 351-360
    • Altamirano, C.1    Cairo, J.J.2    Godia, F.3
  • 38
    • 33748300981 scopus 로고    scopus 로고
    • Considerations on the lactate consumption by CHO cells in the presence of galactose
    • Altamirano C., Illanes A., Becerra S., Cairo J.J., Godia F. Considerations on the lactate consumption by CHO cells in the presence of galactose. J Biotechnol 2006, 125:547-556.
    • (2006) J Biotechnol , vol.125 , pp. 547-556
    • Altamirano, C.1    Illanes, A.2    Becerra, S.3    Cairo, J.J.4    Godia, F.5
  • 39
    • 78649410664 scopus 로고    scopus 로고
    • Metabolic flux analysis of CHO cell metabolism in the late non-growth phase
    • Sengupta N., Rose S.T., Morgan J.A. Metabolic flux analysis of CHO cell metabolism in the late non-growth phase. Biotechnol Bioeng 2011, 108:82-92.
    • (2011) Biotechnol Bioeng , vol.108 , pp. 82-92
    • Sengupta, N.1    Rose, S.T.2    Morgan, J.A.3
  • 42
    • 80053035284 scopus 로고    scopus 로고
    • AMP-activated protein kinase: an energy sensor that regulates all aspects of cell function
    • Hardie D.G. AMP-activated protein kinase: an energy sensor that regulates all aspects of cell function. Genes Dev 2011, 25:1895-1908.
    • (2011) Genes Dev , vol.25 , pp. 1895-1908
    • Hardie, D.G.1
  • 43
    • 0033587379 scopus 로고    scopus 로고
    • Metabolic flux analysis of hybridoma continuous culture steady state multiplicity
    • Follstad B.D., Balcarcel R.R., Stephanopoulos G., Wang D.I. Metabolic flux analysis of hybridoma continuous culture steady state multiplicity. Biotechnol Bioeng 1999, 63:675-683.
    • (1999) Biotechnol Bioeng , vol.63 , pp. 675-683
    • Follstad, B.D.1    Balcarcel, R.R.2    Stephanopoulos, G.3    Wang, D.I.4
  • 44
    • 0034606665 scopus 로고    scopus 로고
    • Multiple steady states with distinct cellular metabolism in continuous culture of mammalian cells
    • Europa A.F., Gambhir A., Fu P.C., Hu W.S. Multiple steady states with distinct cellular metabolism in continuous culture of mammalian cells. Biotechnol Bioeng 2000, 67:25-34.
    • (2000) Biotechnol Bioeng , vol.67 , pp. 25-34
    • Europa, A.F.1    Gambhir, A.2    Fu, P.C.3    Hu, W.S.4
  • 45
    • 33749251791 scopus 로고    scopus 로고
    • Fedbatch culture and dynamic nutrient feeding
    • Wlaschin K.F., Hu W.S. Fedbatch culture and dynamic nutrient feeding. Adv Biochem Eng Biotechnol 2006, 101:43-74.
    • (2006) Adv Biochem Eng Biotechnol , vol.101 , pp. 43-74
    • Wlaschin, K.F.1    Hu, W.S.2
  • 46
    • 79954613316 scopus 로고    scopus 로고
    • High-end pH-controlled delivery of glucose effectively suppresses lactate accumulation in CHO fed-batch cultures
    • Gagnon M., Hiller G., Luan Y.T., Kittredge A., DeFelice J., Drapeau D. High-end pH-controlled delivery of glucose effectively suppresses lactate accumulation in CHO fed-batch cultures. Biotechnol Bioeng 2011, 108:1328-1337.
    • (2011) Biotechnol Bioeng , vol.108 , pp. 1328-1337
    • Gagnon, M.1    Hiller, G.2    Luan, Y.T.3    Kittredge, A.4    DeFelice, J.5    Drapeau, D.6
  • 47
    • 0029059493 scopus 로고
    • High viable cell concentration fed-batch cultures of hybridoma cells through on-line nutrient feeding
    • Zhou W., Rehm J., Hu W.S. High viable cell concentration fed-batch cultures of hybridoma cells through on-line nutrient feeding. Biotechnol Bioeng 1995, 46:579-587.
    • (1995) Biotechnol Bioeng , vol.46 , pp. 579-587
    • Zhou, W.1    Rehm, J.2    Hu, W.S.3
  • 48
    • 0030916886 scopus 로고    scopus 로고
    • Alteration of mammalian cell metabolism by dynamic nutrient feeding
    • Zhou W., Rehm J., Europa A., Hu W.S. Alteration of mammalian cell metabolism by dynamic nutrient feeding. Cytotechnology 1997, 24:99-108.
    • (1997) Cytotechnology , vol.24 , pp. 99-108
    • Zhou, W.1    Rehm, J.2    Europa, A.3    Hu, W.S.4
  • 49
  • 50
    • 0035808169 scopus 로고    scopus 로고
    • Engineering of a mammalian cell line for reduction of lactate formation and high monoclonal antibody production
    • Chen K., Liu Q., Xie L., Sharp P.A., Wang D.I. Engineering of a mammalian cell line for reduction of lactate formation and high monoclonal antibody production. Biotechnol Bioeng 2001, 72:55-61.
    • (2001) Biotechnol Bioeng , vol.72 , pp. 55-61
    • Chen, K.1    Liu, Q.2    Xie, L.3    Sharp, P.A.4    Wang, D.I.5
  • 53
    • 84875268975 scopus 로고    scopus 로고
    • From gene switches to mammalian designer cells: present and future prospects
    • Auslander S., Fussenegger M. From gene switches to mammalian designer cells: present and future prospects. Trends Biotechnol 2013, 31:155-168.
    • (2013) Trends Biotechnol , vol.31 , pp. 155-168
    • Auslander, S.1    Fussenegger, M.2
  • 54
    • 0032937141 scopus 로고    scopus 로고
    • Modification of glucose and glutamine metabolism in hybridoma cells through metabolic engineering
    • Paredes C., Prats E., Cairo J.J., Azorin F., Cornudella L., Godia F. Modification of glucose and glutamine metabolism in hybridoma cells through metabolic engineering. Cytotechnology 1999, 30:85-93.
    • (1999) Cytotechnology , vol.30 , pp. 85-93
    • Paredes, C.1    Prats, E.2    Cairo, J.J.3    Azorin, F.4    Cornudella, L.5    Godia, F.6
  • 55
    • 34547518231 scopus 로고    scopus 로고
    • Engineering cell metabolism for high-density cell culture via manipulation of sugar transport
    • Wlaschin K.F., Hu W.S. Engineering cell metabolism for high-density cell culture via manipulation of sugar transport. J Biotechnol 2007, 131:168-176.
    • (2007) J Biotechnol , vol.131 , pp. 168-176
    • Wlaschin, K.F.1    Hu, W.S.2
  • 56
    • 0035930949 scopus 로고    scopus 로고
    • Blocking of acidosis-mediated apoptosis by a reduction of lactate dehydrogenase activity through antisense mRNA expression
    • Jeong D., Kim T.S., Lee J.W., Kim K.T., Kim H.J., Kim I.H., Kim I.Y. Blocking of acidosis-mediated apoptosis by a reduction of lactate dehydrogenase activity through antisense mRNA expression. Biochem Biophys Res Commun 2001, 289:1141-1149.
    • (2001) Biochem Biophys Res Commun , vol.289 , pp. 1141-1149
    • Jeong, D.1    Kim, T.S.2    Lee, J.W.3    Kim, K.T.4    Kim, H.J.5    Kim, I.H.6    Kim, I.Y.7
  • 57
    • 33846643485 scopus 로고    scopus 로고
    • Down-regulation of lactate dehydrogenase-A by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietin
    • Kim S.H., Lee G.M. Down-regulation of lactate dehydrogenase-A by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietin. Appl Microbiol Biotechnol 2007, 74:152-159.
    • (2007) Appl Microbiol Biotechnol , vol.74 , pp. 152-159
    • Kim, S.H.1    Lee, G.M.2
  • 58
    • 79955540196 scopus 로고    scopus 로고
    • Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases
    • Zhou M., Crawford Y., Ng D., Tung J., Pynn A.F., Meier A., Yuk I.H., Vijayasankaran N., Leach K., Joly J., et al. Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases. J Biotechnol 2011, 153:27-34.
    • (2011) J Biotechnol , vol.153 , pp. 27-34
    • Zhou, M.1    Crawford, Y.2    Ng, D.3    Tung, J.4    Pynn, A.F.5    Meier, A.6    Yuk, I.H.7    Vijayasankaran, N.8    Leach, K.9    Joly, J.10
  • 59
    • 0037186256 scopus 로고    scopus 로고
    • Expression of recombinant cytoplasmic yeast pyruvate carboxylase for the improvement of the production of human erythropoietin by recombinant BHK-21 cells
    • Irani N., Beccaria A.J., Wagner R. Expression of recombinant cytoplasmic yeast pyruvate carboxylase for the improvement of the production of human erythropoietin by recombinant BHK-21 cells. J Biotechnol 2002, 93:269-282.
    • (2002) J Biotechnol , vol.93 , pp. 269-282
    • Irani, N.1    Beccaria, A.J.2    Wagner, R.3
  • 60
    • 0033385223 scopus 로고    scopus 로고
    • Improvement of the primary metabolism of cell cultures by introducing a new cytoplasmic pyruvate carboxylase reaction
    • Irani N., Wirth M., van Den Heuvel J., Wagner R. Improvement of the primary metabolism of cell cultures by introducing a new cytoplasmic pyruvate carboxylase reaction. Biotechnol Bioeng 1999, 66:238-246.
    • (1999) Biotechnol Bioeng , vol.66 , pp. 238-246
    • Irani, N.1    Wirth, M.2    van Den Heuvel, J.3    Wagner, R.4
  • 61
    • 0037274314 scopus 로고    scopus 로고
    • Improving glucose and glutamine metabolism of human HEK 293 and Trichoplusia ni insect cells engineered to express a cytosolic pyruvate carboxylase enzyme
    • Elias C.B., Carpentier E., Durocher Y., Bisson L., Wagner R., Kamen A. Improving glucose and glutamine metabolism of human HEK 293 and Trichoplusia ni insect cells engineered to express a cytosolic pyruvate carboxylase enzyme. Biotechnol Prog 2003, 19:90-97.
    • (2003) Biotechnol Prog , vol.19 , pp. 90-97
    • Elias, C.B.1    Carpentier, E.2    Durocher, Y.3    Bisson, L.4    Wagner, R.5    Kamen, A.6
  • 62
    • 80052034701 scopus 로고    scopus 로고
    • Enhanced glycoprotein production in HEK-293 cells expressing pyruvate carboxylase
    • Henry O., Durocher Y. Enhanced glycoprotein production in HEK-293 cells expressing pyruvate carboxylase. Metab Eng 2011, 13:499-507.
    • (2011) Metab Eng , vol.13 , pp. 499-507
    • Henry, O.1    Durocher, Y.2
  • 63
    • 1842425836 scopus 로고    scopus 로고
    • Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells
    • Fogolin M.B., Wagner R., Etcheverrigaray M., Kratje R. Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells. J Biotechnol 2004, 109:179-191.
    • (2004) J Biotechnol , vol.109 , pp. 179-191
    • Fogolin, M.B.1    Wagner, R.2    Etcheverrigaray, M.3    Kratje, R.4
  • 64
    • 34548043957 scopus 로고    scopus 로고
    • Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44)
    • Kim S.H., Lee G.M. Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44). Appl Microbiol Biotechnol 2007, 76:659-665.
    • (2007) Appl Microbiol Biotechnol , vol.76 , pp. 659-665
    • Kim, S.H.1    Lee, G.M.2
  • 65
    • 14744283551 scopus 로고
    • High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker
    • Bebbington C.R., Renner G., Thomson S., King D., Abrams D., Yarranton G.T. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Biotechnology (N Y) 1992, 10:169-175.
    • (1992) Biotechnology (N Y) , vol.10 , pp. 169-175
    • Bebbington, C.R.1    Renner, G.2    Thomson, S.3    King, D.4    Abrams, D.5    Yarranton, G.T.6
  • 66
    • 11944265249 scopus 로고
    • High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification
    • Cockett M.I., Bebbington C.R., Yarranton G.T. High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification. Biotechnology (N Y) 1990, 8:662-667.
    • (1990) Biotechnology (N Y) , vol.8 , pp. 662-667
    • Cockett, M.I.1    Bebbington, C.R.2    Yarranton, G.T.3
  • 67
    • 0034714090 scopus 로고    scopus 로고
    • Expression of carbamoyl phosphate synthetase I and ornithine transcarbamoylase genes in Chinese hamster ovary dhfr-cells decreases accumulation of ammonium ion in culture media
    • Park H., Kim I.H., Kim I.Y., Kim K.H., Kim H.J. Expression of carbamoyl phosphate synthetase I and ornithine transcarbamoylase genes in Chinese hamster ovary dhfr-cells decreases accumulation of ammonium ion in culture media. J Biotechnol 2000, 81:129-140.
    • (2000) J Biotechnol , vol.81 , pp. 129-140
    • Park, H.1    Kim, I.H.2    Kim, I.Y.3    Kim, K.H.4    Kim, H.J.5
  • 68
    • 0028171092 scopus 로고
    • Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells
    • Pendse G.J., Bailey J.E. Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells. Biotechnol Bioeng 1994, 44:1367-1370.
    • (1994) Biotechnol Bioeng , vol.44 , pp. 1367-1370
    • Pendse, G.J.1    Bailey, J.E.2
  • 69
    • 65549153512 scopus 로고    scopus 로고
    • Expression of anti-apoptosis genes alters lactate metabolism of Chinese Hamster Ovary cells in culture
    • Dorai H., Kyung Y.S., Ellis D., Kinney C., Lin C., Jan D., Moore G., Betenbaugh M.J. Expression of anti-apoptosis genes alters lactate metabolism of Chinese Hamster Ovary cells in culture. Biotechnol Bioeng 2009, 103:592-608.
    • (2009) Biotechnol Bioeng , vol.103 , pp. 592-608
    • Dorai, H.1    Kyung, Y.S.2    Ellis, D.3    Kinney, C.4    Lin, C.5    Jan, D.6    Moore, G.7    Betenbaugh, M.J.8


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