Engineering of a mammalian cell line for reduction of lactate formation and high monoclonal antibody production

Biotechnology and Bioengineering

Volumn 72, Issue 1, 2001, Pages 55-61

  Chen, Keqin ^a   Liu, Qian ^b   Xie, Liangzhi ^c   Sharp, Phillip A ^a   Wang, Daniel I C ^a,d  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b China National Center for Biotechnology Development   (China)

^c MERCK RESEARCH LABORATORIES   (United States)

^d DUPONT COMPANY   (United States)

Author keywords

Antibody production; Hybridoma cell; Knockout; Lactate; LDH gene

Indexed keywords

LACTIC ACID; MONOCLONAL ANTIBODY;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; BIOENGINEERING; CELL DENSITY; CELL GROWTH; CELL VIABILITY; MOUSE; NONHUMAN;

AMMONIA; ANIMALS; ANTIBODIES, MONOCLONAL; CELL DIVISION; DNA; GENETIC ENGINEERING; GENETIC VECTORS; HYBRIDOMAS; L-LACTATE DEHYDROGENASE; LACTATES; MAMMALS; MICE; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; TRANSFECTION;

ANIMALIA; MAMMALIA; TRIXIS;

AMMONIA; DNA; ENZYMES; GENES; GLUCOSE; MONOCLONAL ANTIBODIES; SYNTHESIS (CHEMICAL);

HYBRIDOMA CELLS; LACTATE;

ANIMAL CELL CULTURE;

EID: 0035808169     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20010105)72:1<55::AID-BIT8>3.0.CO;2-4     Document Type: Article

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