Down-regulation of lactate dehydrogenase-A by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietin

Applied Microbiology and Biotechnology

Volumn 74, Issue 1, 2007, Pages 152-159

  Kim, Sung Hyun ^a   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Chinese hamster ovary (CHO) cells; Lactate dehydrogenase A (LDH A); Small interfering RNA (siRNA); Specific lactate production rate

Indexed keywords

CARBOXYLIC ACIDS; CELL CULTURE; CELLS; CONCENTRATION (PROCESS); DRUG PRODUCTS; GROWTH KINETICS; METABOLISM; RNA;

CELLULAR METABOLISM; CHINESE HAMSTER OVARY (CHO) CELLS; LACTATE DEHYDROGENASE-A (LDH-A); SMALL INTERFERING RNA (SIRNA);

ENZYMES;

LACTATE DEHYDROGENASE; LACTIC ACID; PYRUVIC ACID; SMALL INTERFERING RNA; THROMBOPOIETIN;

CELLS AND CELL COMPONENTS; CLONE; DRUG; ENZYME; ENZYME ACTIVITY; GENE EXPRESSION; METABOLISM; RNA; RODENT;

ANIMAL CELL; ARTICLE; CATALYSIS; CELL CLONING; CELL CULTURE; CELL PROLIFERATION; CHO CELL; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; GLUCOSE INTAKE; HOMOLOGOUS RECOMBINATION; METHODOLOGY; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; BIOTECHNOLOGY; CHO CELLS; CRICETINAE; CRICETULUS; DOWN-REGULATION; GENETIC ENGINEERING; HUMANS; L-LACTATE DEHYDROGENASE; LACTIC ACID; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING; THROMBOPOIETIN; TRANSFECTION;

CRICETULUS GRISEUS; MAMMALIA;

EID: 33846643485     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0654-5     Document Type: Article

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