Metabolic flux analysis of hybridoma continuous culture steady state multiplicity

Biotechnology and Bioengineering

Volumn 63, Issue 6, 1999, Pages 675-683

  Follstad, Brian D ^a   Balcarcel, R Robert ^a   Stephanopoulos, Gregory ^a   Wang, Daniel I C ^a  

^a Massachusetts Institute of Technology   (United States)

Author keywords

Cell culture; Central metabolism; Chemostat culture; Mammalian cells; Metabolic flux

Indexed keywords

AMINES; ANIMAL CELL CULTURE; CHEMOSTATS; ENZYME KINETICS; GENETIC ENGINEERING; GROWTH KINETICS; METABOLISM; MUTAGENESIS; PHYSIOLOGY;

CENTRAL METABOLISM; CHEMOSTAT CULTURE; GENETIC MUTATIONS; HYBRIDOMA; INNOCULATION; MAMMALIAN CELLS; METABOLIC FLUX ANALYSIS; STEADY STATE MULTIPLICITY;

CONTINUOUS CELL CULTURE;

ADAPTATION; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CELL METABOLISM; CELL VIABILITY; CHEMOSTAT; DILUTION; ENERGY TRANSFER; GENE MUTATION; HYBRIDOMA; MAMMAL CELL; NONHUMAN; STEADY STATE;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL SURVIVAL; CELLS, CULTURED; CITRIC ACID CYCLE; ENERGY METABOLISM; HUMANS; HYBRIDOMAS; IMMUNOGLOBULIN G; MICE; PYRUVIC ACID;

ANIMALIA; MAMMALIA;

EID: 0033587379     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990620)63:6<675::AID-BIT5>3.0.CO;2-R     Document Type: Article

References (33)

1. Avgerinos GC, Drapeau D, Socolow JS, Mao J-I, Hsiao K, Broeze RJ. 1990. Spin filter perfusion system for high density cell culture: Production of recombinant urinary type plasminogen activator in CHO cells. Bio/Technology 8:54-58.
2. Banik GG, Heath CA. 1995. Partial and total cell retention in a filtration-based homogeneous perfusion reactor. Biotechnol Prog 11:584-588.
3. Batt BC, Davis RH, Kompala DS. 1990. Inclined sedimentation for selective retention of viable hybridomas in a continuous suspension bioreactor. Biotechnol Prog 6:458-464.
4. Bibila TA, Ranucci CS, Glazomitsky K, Buckland BC, Aunins JG. 1994. Monoclonal antibody process development using medium concentrates. Biotechnol Prog 10:87-96.
5. Bonarius HPJ, Hatzimanikatis V, Meesters KPH, de Gooijer CD, Schmid G, Tramper J. 1996. Metabolic flux analysis of hybridoma cells in different culture media using mass balances. Biotechnol Bioeng 50: 299-318.
6. Bonarius HPJ, Houtman JHM, de Gooijer CD, Tramper J, Schmid G. 1998. Activity of glutamate dehydrogenase is increased in ammonia-stressed hybridoma cells. Biotechnol Bioeng 57:447-453.
7. Chung JD, Sinskey AJ, Stephanopoulos G. 1998. Growth factor and bcl-2 mediated survival during abortive proliferation of hybridoma cell line. Biotechnol Bioeng 57:164-171.
8. Dykhuizen DE, Hartl DL. 1983. Selection in chemostats. Microbiol Rev 47:150-168.
9. Eigenbrodt E, Fister P, Reinacher M. 1985. New perspectives on carbohydrate metabolism in tumor cells. In: Beitner R, editor. Regulation of carbohydrate metabolism. Boca Raton, FL: CRC Press, Inc. p 141-179.
10. Follstad BD, Stephanopoulos G. 1998. Effect of reversible reactions on isotope label redistribution. Analysis of the pentose phosphate pathway. Eur J Biochem 252:360-371.
11. Hayter PM, Curling EMA, Baines AJ, Jenkins N, Salmon I, Strange PG, Tong JM, Bull AT. 1992. Glucose-limited chemostat culture of Chinese hamster ovary cells producing recombinant human Interferon-γ. Biotechnol Bioeng 39:327-335.
12. Hayter PM, Curling EMA, Gould ML, Baines AJ, Jenkins N, Salmon I, Strange PG, Bull AT. 1993. The effect of the dilution rate on CHO cell physiology and recombinant interferon-γ production in glucose-limited chemostat culture. Biotechnol Bioeng 42:1077-1085.
13. Lee Y-K, Yap P-K, Teoh A-P. 1995. Correlation between steady-state cell concentration and cell death of hybridoma cultures in chemostat. Biotechnol Bioeng 45:18-26.
14. Linardos TI, Kalogerakis N, Behie LA, Lamontagne LR. 1991. The effect of specific growth rate and death rate on monoclonal antibody production in hybridoma chemostat cultures. Can J Chem Eng 69: 429-438.
15. Mercille S, Massie B. 1994. Induction of apoptosis in nutrient-deprived cultures of hybridoma and myeloma cells. Biotechnol Bioeng 44: 1140-1154.
16. Miller WM, Blanch HW, Wilke CR. 1988. A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: Effect of nutrient concentration, dilution rate, and pH. Biotechnol Bioeng 32:947-965.
17. Mosser DD, Massie B. 1994. Genetically engineering mammalian cell lines for increased viability and productivity. Biotechnol Adv 12:253-277.
18. Nyberg GB, Balcarcel RR, Follstad BD, Stephanopoulos G, Wang DIC. 1999. Metabolism of peptide amino acids by Chinese hamster ovary cells grown in a complex medium. Biotechnol Bioeng, 62:324-335.
19. 13C]glucose incubation. Eur J Biochem 217:457-468.
20. Pörtner R, Schäfer T. 1996. Modelling hybridoma cell growth and metabolism: A comparison of selected models and data. J Biotechnol 49: 119-135.
21. Renner WA, Lee KH, Hatzimanikatis V, Bailey JE, Eppenberger HM. 1995. Recombinant cyclin E expression activates proliferation and obviates surface attachment of Chinese hamster ovary (CHO) cells in protein-free medium. Biotechnol Bioeng 47:476-482.
22. Sharfstein ST, Tucker SN, Mancuso A, Blanch HW, Clark DS. 1994. Quantitative in vivo nuclear magnetic resonance studies of hybridoma metabolism. Biotechnol Bioeng 43:1059-1074.
23. Simpson TW, Follstad BD, Stephanopoulos G. 1999. Analysis of the pathway structure of metabolic networks. J Biotechnol, in press.
24. Vallino JJ, Stephanopoulos G. 1993. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol Bioeng 41:633-646.
25. Vriezen N, van Dijken JP. 1998. Fluxes and enzyme activities in central metabolism of myeloma cells grown in chemostat culture. Biotechnol Bioeng 59:28-39.
26. Wang NS. Stephanopoulos G. 1983. Application of macroscopic balances to the identification of gross measurement errors. Biotechnol Bioeng 25:2177-2208.
27. Wiechert W, de Graaf AA. 1997a. Bidirectional reaction steps in metabolic networks. I. Modeling and simulation of carbon isotope labeling experiments. Biotechnol Bioeng 55:101-117.
28. Wiechert W, Siefke C, de Graaf AA, Marx A. 1997b. Bidirectional reaction steps in metabolic networks. II. Flux estimation and statistical analysis. Biotechnol Bioeng 55:118-135.
29. Xie L, Wang DIC. 1994. Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor. Cytotechnology 15:17-29.
30. Zeng A-P, Deckwer W-D, Hu W-S. 1998. Determinants and rate laws of growth and death of hybridoma cells in continuous culture. Biotechnol Bioeng 57:642-654.
31. Zhou W, Rehm J, Hu W-S. 1995. High viable cell concentration fed-batch cultures of hybridoma cells through on-line nutrient feeding. Biotechnol Bioeng 46:579-587.
32. Zupke C, Sinskey AJ, Stephanopoulos G. 1995. Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl Microbiol Biotechnol 44:27-36.
33. 13C NMR. Biotechnol Bioeng 45:292-303.