Peak antibody production is associated with increased oxidative metabolism in an industrially relevant fed-batch CHO cell culture

Biotechnology and Bioengineering

Volumn 110, Issue 7, 2013, Pages 2013-2024

  Templeton, Neil ^a   Dean, Jason ^b   Reddy, Pranhitha ^b   Young, Jamey D ^a  

^a VANDERBILT UNIVERSITY   (United States)

^b AMGEN INC   (United States)

Author keywords

Aerobic glycolysis; Antibody production; Chinese hamster ovary (CHO); Fed batch; Lactate switch; Metabolic flux analysis (MFA)

Indexed keywords

AEROBIC GLYCOLYSIS; ANTIBODY PRODUCTION; CHINESE HAMSTER OVARY; FED BATCHES; METABOLIC FLUX ANALYSIS;

BATCH CELL CULTURE; METABOLISM; PHYSIOLOGY;

ANTIBODIES;

CARBON 13; LACTIC ACID;

AEROBIC METABOLISM; ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; BIOTECHNOLOGY; CELL DENSITY; CELL GROWTH; CELL METABOLISM; CHO CELL; CITRIC ACID CYCLE; FED BATCH CULTURE; GLYCOLYSIS; GROWTH RATE; ISOTOPE LABELING; METABOLIC FLUX ANALYSIS; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PENTOSE PHOSPHATE CYCLE; PROCEDURES; STATISTICAL ANALYSIS;

ANIMALS; ANTIBODIES; CARBON RADIOISOTOPES; CELL RESPIRATION; CHO CELLS; CRICETULUS; ENERGY METABOLISM; ISOTOPE LABELING; LACTATES; METABOLIC FLUX ANALYSIS; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS;

EID: 84878388792     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24858     Document Type: Article

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