Enhanced glycoprotein production in HEK-293 cells expressing pyruvate carboxylase

Metabolic Engineering

Volumn 13, Issue 5, 2011, Pages 499-507

  Henry, Olivier ^a   Durocher, Yves ^b  

^a ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

13C metabolic flux analysis; HEK 293 cells; Pyruvate carboxylase; Recombinant interferon

Indexed keywords

AMMONIA PRODUCTION; BATCH CULTURE; CARBON METABOLISM; CELL DENSITY; EXPRESSION SYSTEM; FED-BATCH PROCESS; FLUX ANALYSIS; HEK293 CELLS; HIGH QUALITY; ISOTOPOMER DISTRIBUTIONS; MAXIMUM SPECIFIC GROWTH RATES; METABOLIC CHANGES; PARENTAL CELLS; PRODUCTION YIELD; PYRUVATE CARBOXYLASE; QUANTITATIVE KNOWLEDGE; VIABLE CELLS;

ANTIBIOTICS; BATCH CELL CULTURE; BATCH DATA PROCESSING; CLONING; GENES; GLYCOPROTEINS; ISOMERS; ISOTOPES; MANUFACTURE; METABOLISM; PHYSIOLOGY;

CLONE CELLS;

ALBINTERFERON ALPHA2B; AMMONIA; CARBON 13; LACTIC ACID; PYRUVATE CARBOXYLASE;

ARTICLE; BACTERIAL GROWTH; CARBON METABOLISM; CELL CLONE; CELL DENSITY; CELL STRAIN HEK293; CONTROLLED STUDY; FED BATCH CULTURE; GROWTH RATE; HUMAN; HUMAN CELL; METABOLITE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

CELL SURVIVAL; GLYCOPROTEINS; HEK293 CELLS; HUMANS; INTERFERON ALFA-2B; PYRUVATE CARBOXYLASE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 80052034701     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2011.05.004     Document Type: Article

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