Energy metabolism and ATP balance in animal cell cultivation using a stoichiometrically based reaction network

Biotechnology and Bioengineering

Volumn 52, Issue 5, 1996, Pages 591-601

  Xie, Liangzhi ^a   Wang, Daniel I C ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

animal cell metabolism; ATP balance; energy metabolism; hybridoma; reaction network; stoichiometric analysis

Indexed keywords

ADENOSINETRIPHOSPHATE; ANIMAL CELL CULTURE; BATCH CELL CULTURE; BIOTECHNOLOGY; CELLS; ENZYMES; ESTIMATION; FREE ENERGY; MATHEMATICAL MODELS; OXYGEN; STOICHIOMETRY; SYNTHESIS (CHEMICAL);

ANIMAL CELL METABOLISM; ENERGY METABOLISM; FRACTIONAL MODEL; HYBRIDOMA; OXIDATIVE PHOSPHORYLATION; OXYGEN UPTAKE RATE; REACTION NETWORK; RESPIRATORY QUOTIENT;

METABOLISM;

ADENOSINE TRIPHOSPHATE;

ANIMAL CELL; ARTICLE; CELL METABOLISM; ENERGY METABOLISM; GLUCOSE METABOLISM; MATHEMATICAL MODEL; NONHUMAN; OXYGEN CONSUMPTION; STOICHIOMETRY;

EID: 0030571616     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19961205)52:5<591::AID-BIT6>3.0.CO;2-E     Document Type: Article

References (39)

1. Bailey, J. E., Ollis, D. F. 1977. Biochemical engineering fundamentals. McGraw-Hill, New York.
2. Bauchop, T., Elsden, S. R. 1960. The growth of micro-organisms in relation to their energy supply. J. Gen. Microbiol. 23: 457-469.
3. Brand, M. D., Happer, M. E., Taylor, H. C. 1993. Control of the effective P/0 ratio of oxidative phosphorylation in liver mitochondria and hepatocytes. Biochem. J. 291: 739-748.
4. Cortassa, S., Aon, J. C., Aon, M. A. 1995. Fluxes of carbon, phosphorylation, and redox intermediates during growth of Saccharomyces cerevisiae on different carbon sources. Biotechnol. Bioeng. 47: 193-208.
5. Glacken, M. W., Fleischaker, R. J., SInskey, A. J. 1986. Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells. Biotechnol. Bioeng. 28: 1376-1389.
6. Goel, A., Ferrance, J., Jeong, J., Ataai, M. M. 1993. Analysis of metabolic fluxes in batch and continuous culture of Bacillus subtilis. Biotechnol. Bioeng. 42: 686-696.
7. Hinkle, P. C., Kumar, M. A., Resetar, A., Harris, D. L. 1991. Mechanistic stoichiometry of mitochondrial oxidative phosphorylation. Biochemistry 30: 3576-3582.
8. Lazo, P. A. 1981. Amino acids and glucose utilization by different metabolic pathways. Eur. J. Biochem. 117: 19-25.
9. Mckeehan, W. L. 1986. Glutaminolysis in animal cells, pp. 111-150. In: M. J. Morgan (ed.), Carbohydrate metabolism in cultured cells. Plenum, New York.
10. Mancuso, A., Sharfstein, S. T., Tucker, S. N., Clark, D. S., Blanch, H. W. 1994. Examination of primary metabolic pathways in a murine hybridoma with carbon-13 nuclear magnetic resonance spectroscopy. Biotechnol. Bioeng. 44: 563-585.
11. Miller, W. M., Wilke, C. R., Blanch, H. W. 1989a. Transient responses of hybridoma cells to nutrient additions in continuous culture: I. Glucose pulse and step changes. Biotechnol. Bioeng. 33: 477-486.
12. Miller, W. M., Wilke, C. R., Blanc, H. W. 1989b. The transient responses of hybridoma cells to nutrient additions in continuous culture: II. Glutamine pulse and step changes. Biotechnol Bioeng. 33: 487-499.
13. Nobes, C. D., Brown, G. C., Oliver, P. N., Brand, M. D. 1990. Non-ohmic proton conductance of the mitochondrial inner membrane in hepatocytes. J. Biol. Chem. 265: 12903-12909.
14. Ozturk, S. S., Palsson, B. O. 1991a. Growth, metabolic, and antibody production kinetics of hybridoma cell culture: 1. Analysis of data from controlled batch reactors. Biotechnol. Prog. 7: 471-480.
15. Ozturk, S. S., Palsson, B. O. 1991b. Growth, metabolic, and antibody production kinetics of hybridoma cell culture: 2. Effects of serum concentration, dissolved oxygen concentration, and medium pH in a batch reactor. Biotechnol. Prog. 7: 481-494.
16. Papoutsakis, E. T. 1984. Equations and calculations for fermentations of butyric acid bacteria. Biotechnol. Bioeng. 26: 174-187.
17. Papoutsakis, E. T., Charles, L. M. 1985. Fermentation equations for propionic-acid bacteria and production of assorted oxychemicals from various sugars. Biotechnol. Bioeng. 27: 67-80.
18. Papoutsakis, E. T., Meyer, C. L. 1985. Equations and calculations of product yields and preferred pathways for butanediol and mixed-acid fermentations. Biotechnol. Bioeng. 27: 50-66.
19. Paul, J. 1965. Carbohydrate and energy metabolism, pp. 239-276. In: E. N. Willmer (ed.), Cells and tissues in culture, vol. 1. Academic, New York.
20. Roels, J. A. 1980. Simple model for the energetics of growth on substrates with different degrees of reduction. Biotechnol. Bioeng. 22: 33-53.
21. Savinell, J. M., Palsson, B. O. 1992. Network analysis of intermediary metabolism using linear optimization. II. Interpretation of hybridoma cell metabolism. J. Theor. Biol. 154: 455-473.
22. Sharfstein, S. T., Tucker, S. N., Mancuso, A., Blanch, H. W., Clark, D. S. 1994. Quantitative in vivo nuclear magnetic resonance studies of hybridoma metabolism. Biotechnol. Bioeng. 43: 1059-1074.
23. Shuler, M. L., Kargi, F. 1992. Bioprocess engineering: Basic concepts. Prentice-Hall, Englewood Cliffs, NJ.
24. - stoichiometries at the individual coupling sites in mitochondrial oxidative phosphorylation. J. Biol. Chem. 262: 10445-10453.
25. Stouthamer, A. H. 1977. A theoretical study on the amount of ATP required for synthesis of microbial cell material. Ant. Leeuwenh. 39: 545-565.
26. Stouthamer, A. H. 1977. Theoretical calculation on the influence of the inorganic nitrogen source on parameters for aerobic growth of microorganisms. Ant. Leeuwenh. 43: 351-367.
27. Stouthamer, A. H. 1979a. The search for correlation between theoretical and experimental growth yields, pp. 1-47. In: J. R. Quayle (ed.), Microbial biochemistry, vol. 21. University Park Press, Baltimore.
28. Stouthamer, A. H. 1979b. Energy production, growth, and product formation by microorganisms, pp. 70-76. In: O. K. Sebek and A. I. Laskin (eds.), Genetics of industrial microorganisms. American Society for Microbiology, Washington, DC.
29. Stryer, L. 1988. Biochemistry, 3rd edition. W. H. Freeman, New York.
30. Vallino, J. J., Stephanopoulos, G. 1993. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol. Bioeng. 41: 633-646.
31. Xie, L., Wang, D. I. C. 1994a. Stoichiometric analysis of animal cell growth and its application of medium design. Biotechnol. Bioeng. 43: 1164-1174.
32. Xie, L., Wang, D. I. C. 1994b. Fed-batch culotivation of animal cells using different medium design concepts and feeding strategies. Biotechnol. Bioeng. 43: 1175-1189.
33. Xie, L., Wang, D. I. C. 1994c. Applications of improved Stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor. Cytotechnology 15: 17-29.
34. Xie, L. and Wang, D. I. C. 1996. Material balance studies on animal cell metabolism using a stoichiometrically based reaction network. Biotechnol. Bioeng. 52: 579-590.
35. Zhou, W., Rehm, J., Hu, W.-S. 1995. High viable cell concentration fedbatch cultures of hybridoma cells through on-line nutrient feeding. Biotechnol. Bioeng. 46: 579-587.
36. Zielke, H. R., Ziekle, C. L., Ozand, P. T. 1984. Glutamine: A major energy source for cultured mammalian cells. Fed. Proc. 43: 121-125.
37. Zupke, C. 1993. Metabolic flux analysis in mammalian cell culture. Ph.D. thesis, MIT, Cambridge, MA.
38. 13CNMR. Biotechnol. Bioeng. 45: 292-303.
39. Zupke, C., Sinskey, A. J., Stephanopoulos, G. 1995. Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl. Microbiol. Biotechnol., 44: 27-36.