Expression of recombinant cytoplasmic yeast pyruvate carboxylase for the improvement of the production of human erythropoietin by recombinant BHK-21 cells

Journal of Biotechnology

Volumn 93, Issue 3, 2002, Pages 269-282

  Irani, Noushin ^a,b   Beccaria, Alejandro Joseé ^a,c   Wagner, Roland ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

^c UNIVERSIDAD NACIONAL DEL LITORAL   (Argentina)

Author keywords

Erythropoietin; Mammalian cell lines; Metabolic engineering; Pyruvate carboxylase

Indexed keywords

BIOREACTORS; CELLS; GENES; GLUCOSE; YEAST;

BATCH CULTIVATION;

PROTEINS;

ERYTHROPOIETIN; GLUCOSE; GLUTAMINE; LACTIC ACID; PYRUVATE CARBOXYLASE; RECOMBINANT ENZYME;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL CULTURE; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM; GENETIC TRANSFECTION; GLUCOSE INTAKE; GLYCOLYSIS; HAMSTER; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

ANIMALS; BIOREACTORS; CELL LINE; CRICETINAE; CULTURE MEDIA; CYTOPLASM; DNA, RECOMBINANT; ERYTHROPOIETIN, RECOMBINANT; GENETIC VECTORS; GLUTAMINE; HUMANS; PYRUVATE CARBOXYLASE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSFECTION;

MAMMALIA;

EID: 0037186256     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(01)00409-6     Document Type: Article

References (56)

1. The effect of culture conditions on the oligosaccharide structures of recombinant glycoproteins
2. Characterization of two differently processed forms of human recombinant factor IX synthesised in CHO cells transformed with a polycistronic vector
3. Monoclonal antibody process development using medium concentrates
4. Selecting and designing cell lines for improved physiological characteristics
5. Expression of human pancreatic polypeptide precursors from a dicistronic mRNA in mammalian cell
6. Nutrient enrichment and in-situ waste removal through electrical means for hybridoma cultures
7. Study of adenovirus production in serum-free 293SF suspension culture by monitoring GFP-expression
8. Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells
9. Recombinant human interferon-γ. Differences in glycosylation and proteolytic processing lead to heterogeneity in batch culture
10. Dicistronic transcription units for gene expression in mammalian cells
11. N- and O-glycosylation muteins of recombinant human erythropoietin secreted from BHK-21 cells
12. A novel cytostatic process enhances the productivity of Chinese hamster ovary cells
13. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells
14. Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells
15. Expression of pyruvate carboxylase enhances succinate production in Escherichia coli without affecting glucose uptake
16. Construction of stable BHK-21 cells coexpressing human secretory glycoproteins and human Gal(β1-4)GlcNAc-R α-2, 6-sialyltransferase. α2, 6-linked NeuAc is preferentially attached to the Gal(β1-4)-GlcNAc(β1-2)Man(α1-3)-branch of dianntennary oligosaccharides from secreted recombinant β-trace protein
17. Proteolytic cleavage of recombinant twi-chain Factor VIII during cell culture production is mediated by proteases from lysed cells: The use of pulse labeling directly in production medium
18. Improvement of the primary metabolism of cell cultures by introducing a new cytoplasmic pyruvate carboxylase reaction
19. The novel mechanism of initiation of picorna virus RNA translation
20. A segment of the 5′ nontranslated region of encephalomyocarditis virus RNA directs entry of ribosomes during in-vitro translation
21. Glycosylation of recombinant proteins: Problems and prospects
22. Retroviral infection of Syrian hamster cells depends on age and susceptibility towards sialidase
23. Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus
24. Interferon regulatory factor 1 (IRF-1) mediates cell growth inhibition by transactivation of downstream target genes
25. Proliferation control of mammalian cells by the tumor suppressor IRF-1
26. Evaluation of the proteolytic potential of in vitro-cultivated hybridoma and recombinant mammalian cells
27. Effects of ammonium and lactate on growth and metabolism of a recombinant CHO cell culture
28. A method for quantitative amino acid analysis using pre-column o-phthaldialdehyd derivatization and high performance liquid chromatography
29. A rapid method for counting cell nuclei using a particle sizer/counter
30. Catabolic control of hybridoma cells by glucose and glutamine limited fed-batch cultures
31. A novel autoregulated proliferation-controlled production process using recombinant CHO cells
32. Higher productivity of growth-arrested Chinese hamster ovary cells expressing the cyclin-dependent kinase inhibitor p27
33. Internal initiation of translation mediated by the 5′ leader of a cellular mRNA
34. Recombinant glycoprotein product quality in proliferation-controlled BHK-21 cells
35. Structures of sialylated oligosaccharides of human erythropoietin expressed in recombinant BHK-21 cells
36. Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production
37. --cells decreases accumulation of ammonium ion in culture media
38. Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells
39. Human erythropoietin gene: High level expression in stably transfected cells and chromosome localization
40. The biosynthesis of threonine by mammalian cells: Expression of a complete bacterial biosynthethic pathway in an animal cell
41. Recombinant cyclin E expression activates proliferation and obviates surface attachment of Chinese hamster ovary (CHO) cells in protein-free medium
42. Production of recombinant interleukin 2 with BHK cells in a hollow fibre and a stirred tank reactor with protein-free medium
43. A reliable strategy for the achievement of cell lines growing in protein-free medium
44. Poliovirus translation
45. Activation of a specific proteolytic activity in suspension cultures of recombinant adherent cells
46. Effects of glucose on the production of recombinant protein-C in mammalian cell culture, e.g. CHO-B3 cell culture
47. Plasmid maintenance assay based on green fluorescent protein and FACS of mammalian cells
48. The growth and productivity of recombinant animal cells in a bubble-free aeration system
49. Engineering Chinese hamster ovary cells to maximize sialic acid content of recombinant glycoproteins
50. Use of dicistronic transcription units for the correlated expression of two genes in mammalian cells
51. An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells