Strategies to optimize protein expression in E. coli

Current Protocols in Protein Science

Volumn , Issue SUPPL. 61, 2010, Pages

  Francis, Dana M ^a   Page, Rebecca ^a  

^a BROWN UNIVERSITY   (United States)

Author keywords

active protein; E. coli; expression strains and vectors; expression tags; folded protein; fusion proteins; heterologous protein; proteases; protein expression; purification tags

Indexed keywords

ANTITOXIN; BACTERIAL PROTEIN; BACTERIAL TOXIN; CHAPERONE; GLUTATHIONE REDUCTASE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MEMBRANE PROTEIN; MESSENGER RNA; MUTANT PROTEIN; N UTILIZATION SUBSTANCE A; PROTEIN TAG; PROTEIN TYROSINE KINASE; PROTEINASE; RECOMBINANT PROTEIN; SOLUBILITY ENHANCING TAG; SUMO PROTEIN; THIOREDOXIN; THIOREDOXIN REDUCTASE; TRANSFER RNA; UNCLASSIFIED DRUG; HEXAHISTIDINE; PLASMID VECTOR;

AMINO ACID SEQUENCE; BACTERIAL KINETICS; BACTERIAL STRAIN; CLINICAL PROTOCOL; CODON; CONCENTRATION (PARAMETERS); CULTURE MEDIUM; DISULFIDE BOND; ENZYME ACTIVITY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE DOSAGE; GENE EXPRESSION SYSTEM; GENE MUTATION; GENE REPLICATION; GENE SEQUENCE; LOW TEMPERATURE PROCEDURES; MOLECULAR CLONING; MOLECULAR SIZE; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROMOTER REGION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; PROTEIN TARGETING; REVIEW; SEQUENCE ANALYSIS; SOLUBILITY; STRUCTURAL GENOMICS; ARTICLE; CYTOSOL; PROTEIN FUNCTION; PROTEIN MISFOLDING; PROTEIN MODIFICATION; PROTEIN UNFOLDING; TEMPERATURE; VIRUS REPLICATION;

ESCHERICHIA COLI; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEOMICS; RECOMBINANT PROTEINS; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 77956485238     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps0524s61     Document Type: Review

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