Effective high-throughput overproduction of membrane proteins in Escherichia coli

Protein Expression and Purification

Volumn 62, Issue 1, 2008, Pages 1-8

  Gordon, Euan ^a   Horsefield, Rob ^b   Swarts, Herman G P ^c   de Pont, Jan Joep H H M ^c   Neutze, Richard ^b   Snijder, Arjan ^a  

^a CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

^c RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Auto induction; Escherichia coli; His tag; Legionella pneumophila; Legionnaires' disease; Membrane proteins; Protein production; Structural genomics

Indexed keywords

HISTIDINE; HYBRID PROTEIN; ISOPROPYL THIOGALACTOSIDE; MEMBRANE PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; LEGIONELLA PNEUMOPHILA; METABOLISM; MOLECULAR CLONING; PHOSPHORYLATION; PROTEIN ENGINEERING; TEMPERATURE;

CLONING, MOLECULAR; ESCHERICHIA COLI; HISTIDINE; ISOPROPYL THIOGALACTOSIDE; LEGIONELLA PNEUMOPHILA; MEMBRANE PROTEINS; PHOSPHORYLATION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

ESCHERICHIA COLI; LEGIONELLA PNEUMOPHILA;

EID: 52949098698     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.07.005     Document Type: Article

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