One-step affinity purification of the G protein βγ subunits from bovine brain using a histidine-tagged G protein α subunit

Protein Expression and Purification

Volumn 15, Issue 2, 1999, Pages 207-212

  Tanaka, Takeshi ^a,b   Kubota, Makoto ^b   Samizo, Koichi ^b   Nakajima, Yoshitaka ^b   Hoshino, Masao ^b   Kohno, Toshiyuki ^a   Wakamatsu, Kaori ^b,c  

^a MITSUBISHI KASEI INST OF LIFE SCI   (Japan)

^b GUNMA UNIVERSITY   (Japan)

^c INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; GUANINE NUCLEOTIDE BINDING PROTEIN; PROTEIN PURIFICATION;

ANIMALIA; BOVINAE;

EID: 0033102528     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.1011     Document Type: Article

References (32)

1. Kaziro Y., Itoh H., Kozasa T., Nakafuku M., Satoh T. Structure and function of signal-transducing GTP-binding proteins. Annu. Rev. Biochem. 60:1991;349-400.
2. Neer E. J. Heterotrimeric G proteins: Organizers of transmembrane signals. Cell. 80:1995;249-257.
3. Clapham D. E., Neer E. J. G protein βγ subunits. Annu. Rev. Pharmacol. Toxicol. 37:1997;167-203.
4. Sternweis P. C. The active role of βγ in signal transduction. Curr. Opin. Cell Biol. 6:1994;198-203.
5. Inglese J., Koch W. J., Touhara K., Lefkowitz R. J. G βγ interactions with PH domains and Ras-MAPK signaling pathways. Trends Biochem. Sci. 20:1995;151-156.
6. Fukada Y. Prenylation and carboxylmethylation of G-protein γ subunit. Methods Enzymol. 250:1995;91-105.
7. 12. J. Biol. Chem. 270:1995;29469-29475.
8. Northup J. K., Smigel M. D., Sternweis P. C., Gilman A. G. The subunits of the stimulatory regulatory component of adenylate cyclase: Resolution, activity, and properties of the 35,000-dalton (β) subunit. J. Biol. Chem. 258:1983;11361-11368.
9. Sternweis P. C., Pang I.-H. Preparation of G-proteins and their subunits. Receptor-Effector Coupling. 1990;IRL Press, New York. p. 1-30.
10. Asano T., Kamiya N., Morishita R., Kato K. Immunoassay for the βγ subunits of GTP-binding proteins and their regional distribution in bovine brain. J. Biochem. (Tokyo). 103:1988;950-953.
11. Mumby S. M., Casey P. J., Gilman A. G., Gutowski S. G protein γ subunits contain a 20-carbon isoprenoid. Proc. Natl. Acad. Sci. USA. 87:1990;5873-5877.
12. Pang I. H., Smrcka A. V., Sternweis P. C. Synthesis and applications of affinity matrix containing immobilized βγ subunits of G proteins. Methods Enzymol. 237:1994;164-174.
13. Tanaka T., Kohno T., Kinoshita S., Mukai H., Itoh H., Ohya M., Miyazawa T., Higashijima T., Wakamatsu K. α Helix content of G protein α subunit is decreased upon activation by receptor-mimetics. J. Biol. Chem. 273:1998;3247-3252.
14. Lee E., Linder M. E., Gilman A. G. Expression of G-protein α subunits inEscherichia coli. Methods Enzymol. 237:1994;146-164.
15. Ueda N., Iñiguez-Lluhi J. A., Lee E., Smrcka A. V., Robishaw J. D., Gilman A. G. G protein βγ subunits: Simplified purification and properties of novel isoforms. J. Biol. Chem. 269:1994;4388-4395.
16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
17. Schägger H., von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:1987;368-379.
18. Asano T., Morishita R., Ohashi K., Nagahama M., Miyake T., Kato K. Localization of various forms of the γ subunit of G protein in neural and nonneural tissues. J. Neurochem. 64:1995;1267-1273.
19. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
20. Robishaw J. D., Balcueva E. A. A high-temperature transfer procedure for detection of G protein γ subunits by immunoblotting. Anal. Biochem. 208:1993;283-287.
21. oα. Methods Enzymol. 195:1991;315-321.
22. oi. J. Biol. Chem. 261:1986;631-637.
23. Schaffner W., Weissmann C. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal. Biochem. 56:1973;502-514.
24. Sternweis P. C., Robishow J. D. Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain. J. Biol. Chem. 259:1984;13806-13813.
25. Florio V. A., Sternweis P. C. Mechanism of muscarinic receptor action on Go in reconstituted phospholipid vesicles. J. Biol. Chem. 264:1989;3909-3915.
26. Fong H. K. W., Amatruda T. T. III, Birren B. W., Simon M. I. Distinct forms of the β subunit of GTP-binding regulatory proteins identified by molecular cloning. Proc. Natl. Acad. Sci. USA. 84:1987;3792-3796.
27. Morishita R., Fukada Y., Kokame K., Yoshizawa T., Masuda K., Niwa M., Kato K., Asano T. Identification and isolation of common and tissue-specific geranylgeranylated γ subunits of guanine-nucleotide-binding regulatory proteins in various tissues. Eur. J. Biochem. 210:1992;1061-1069.
28. Iñiguez-Lluhi J. A., Simon M., Robishaw J. D., Gilman A. G. G protein βγ subunits synthesized in Sf9 cells: Functional characterization and the significance of prenylation of γ J. Biol. Chem. 267:1992;23409-23417.
29. oα. J. Biol. Chem. 266:1991;4654-4659.
30. sα. J. Biol. Chem. 267:1992;1212-1218.
31. 12z. J. Biol. Chem. 270:1995;1734-1741.
32. Ochs D. Protein contaminants of sodium dodecyl sulfate-polyacrylamide gels. Anal. Biochem. 135:1983;470-474.