Inhibition of tobacco etch virus protease activity by detergents

Protein Expression and Purification

Volumn 27, Issue 1, 2003, Pages 109-114

  Mohanty, Arun K ^a   Simmons, Chad R ^a   Wiener, Michael C ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

DETERGENT; PEPTIDE FRAGMENT; PROTEINASE; PROTEINASE INHIBITOR; TEV PROTEASE;

ARTICLE; ENZYME SPECIFICITY; ENZYMOLOGY; METABOLISM; PLANT VIRUS; SOLUBILITY;

DETERGENTS; ENDOPEPTIDASES; PEPTIDE FRAGMENTS; PLANT VIRUSES; PROTEASE INHIBITORS; SOLUBILITY; SUBSTRATE SPECIFICITY;

EID: 0013091910     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00589-2     Document Type: Article

References (20)

1. J. Nilsson, M. Larsson, S. Stahl, P.A. Nygren, M. Uhlen, Multiple affinity domains for the detection, purification and immobilization of recombinant proteins, J. Mol. Recogn. 9 (1996) 585-594.
2. R. Janknecht, G. de Martynoff, J. Lou, R.A. Hipskind, A. Nordheim, H.G. Stunnenberg, Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus, Proc. Natl. Acad. Sci. USA 88 (1991) 8972-8976.
3. + chelate affinity chromatography of the IIBCGlc subunit, FEBS Lett. 324 (1993) 109-112.
4. F.W. Lau, J.U. Bowie, A method for assessing the stability of a membrane protein, Biochemistry 36 (1997) 5884-5892.
5. A.D. Ferguson, J. Breed, K. Diederichs, W. Welte, J.W. Coulton, An internal affinity-tag for purification and crystallization of the siderophore receptor FhuA, integral outer membrane protein from Escherichia coli K-12, Protein Sci. 7 (1998) 1636-1638.
6. D.C. Webb, A.W. Cripps, A method for the purification and refolding of a recombinant form of the nontypeable Haemophilus influenzae P5 outer membrane protein fused to polyhistidine, Protein Expr. Purif. 15 (1999) 1-7.
7. M.D. Purdy, M.C. Wiener, Expression, purification, and initial structural characterization of YadQ, a bacterial homolog of mammalian ClC chloride channel proteins, FEBS Lett. 466 (2000) 26-28.
8. J. Shanklin, Overexpression and purification of the Escherichia coli inner membrane enzyme acyl-acyl carrier protein synthase in an active form, Protein Expr. Purif. 18 (2000) 355-360.
9. D.B. Smith, K.S. Johnson, Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase, Gene 67 (1988) 31-40.
10. U.C. Vothknecht, C.G. Kannangara, D. von Wettstein, Expression of catalytically active barley glutamyl tRNAGlu reductase in Escherichia coli as a fusion protein with glutathione S-transferase, Proc. Natl. Acad. Sci. USA 93 (1996) 9287-9291.
11. K.R. Sticha, C.A. Sieg, C.P. Bergstrom, P.E. Hanna, C.R. Wagner, Overexpression and large-scale purification of recombinant hamster polymorphic arylamine N-acetyltransferase as a dihydrofolate reductase fusion protein, Protein Expr. Purif. 10 (1997) 141-153.
12. S.I. Choi, H.W. Song, J.W. Moon, B.L. Seong, Recombinant enterokinase light chain with affinity tag: expression from Saccharomyces cerevisiae and its utilities in fusion protein technology, Biotechnol. Bioeng. 75 (2001) 718-724.
13. S. Carr, J. Miller, S.E. Leary, A.M. Bennett, A. Ho, E.D. Williamson, Expression of a recombinant form of the V antigen of Yersinia pestis, using three different expression systems, Vaccine 18 (1999) 153-159.
14. T.D. Parks, K.K. Leuther, E.D. Howard, S.A. Johnston, W.G. Dougherty, Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase, Anal. Biochem. 216 (1994) 413-417.
15. W.G. Dougherty, J.C. Carrington, Expression and function of polyviral gene products, Annu. Rev. Phytopathol. 26 (1988) 123-143.
16. R.B. Kapust, K.M. Routzahn, D.S. Waugh, Processive degradation of nascent polypeptides, triggered by tandem AGA codons, limits the accumulation of recombinant tobacco etch virus protease in Escherichia coli BL21(DE3), Protein Expr. Purif. 24 (2002) 61-70.
17. M. Zulauf, Detergent phenomena in membrane protein crystallization, in: H. Michel (Ed.), Crystallization of Membrane Proteins, CRC Press, Boca Raton, FL, 1991, pp. 53-72.
18. Y.S. Ho, L. Swenson, U. Derewenda, L. Serre, Y. Wei, Z. Dauter, M. Hattori, T. Adachi, J. Aoki, H. Arai, K. Inoue, Z.S. Derewenda, Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer, Nature 385 (1997) 89-93.
19. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.
20. P. Sheffield, S. Garrard, Z. Derewenda, Overcoming expression and purification problems of RhoGDI using a family of parallel expression vectors, Protein Expr. Purif. 15 (1999) 34-39.