Influence of process temperature on recombinant enzyme activity in Escherichia coli fed-batch cultures

Enzyme and Microbial Technology

Volumn 43, Issue 7, 2008, Pages 507-512

  Pinsach, Jaume ^a   de Mas, Carles ^a   López Santín, Josep ^a   Striedner, Gerald ^b   Bayer, Karl ^b  

^a CSIC   (Spain)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Aldolases; Fed batch cultures; Proteolysis; Recombinant protein production; Temperature effect

Indexed keywords

BATCH CELL CULTURE; CELL CULTURE; ENZYMES; ESCHERICHIA COLI; GENE ENCODING; PROTEINS;

ALDOLASE; ALDOLASES; COPY NUMBERS; ENERGY SOURCES; FED BATCHES; FED-BATCH CULTURES; HIGH CELL DENSITIES; HOST CELLS; METALLO PROTEASES; OVEREXPRESSION; PROCESS TEMPERATURES; PROTEIN CONTENTS; PROTEIN DEGRADATIONS; RECOMBINANT ENZYMES; RECOMBINANT PROTEIN PRODUCTION; RECOMBINANT PROTEINS; SHORT TERMS; SPECIFIC ACTIVITIES; SPECIFIC PRODUCTION RATES; TEMPERATURE EFFECT; TIME PERIODS;

ENZYME ACTIVITY;

FRUCTOSE BISPHOSPHATE ALDOLASE; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT ENZYME;

ARTICLE; ENZYME ACTIVITY; ENZYME INDUCTION; ENZYME SYNTHESIS; ESCHERICHIA COLI; FED BATCH CULTURE; NONHUMAN; PLASMID; PROTEIN CONTENT; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN QUALITY; TEMPERATURE SENSITIVITY;

ESCHERICHIA COLI;

EID: 55949124415     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2008.08.007     Document Type: Article

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