A systematic approach for testing expression of human full-length proteins in cell-free expression systems

BMC Biotechnology

Volumn 7, Issue , 2007, Pages

  Langlais, Claudia ^a   Guilleaume, Birgit ^b   Wermke, Nadja ^b   Scheuermann, Tina ^b   Ebert, Lars ^b   LaBaer, Joshua ^c   Korn, Bernhard ^d  

^a MRC TOXICOLOGY UNIT   (United Kingdom)

^b German Genome Resource Center RZPD   (Germany)

^c HARVARD MEDICAL SCHOOL   (United States)

^d GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CYTOLOGY; ESCHERICHIA COLI; SOLUBILITY;

CELL-FREE EXPRESSION SYSTEMS; EUKARYOTIC WHEAT GERM SYSTEM; PLASMIDS;

PROTEINS;

PLASMID DNA; ESCHERICHIA COLI PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CELL FREE SYSTEM; COMPARTMENT MODEL; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENOME SIZE; IN VITRO STUDY; IN VIVO STUDY; INTERMETHOD COMPARISON; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLASMID; POLYMERASE CHAIN REACTION; PROCESS OPTIMIZATION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEOMICS; SEQUENCE ANALYSIS; SYSTEMS BIOLOGY; UNINDEXED SEQUENCE; WHEAT GERM; GENE EXPRESSION PROFILING; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PHYSIOLOGY;

ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA; TRITICUM AESTIVUM;

CELL-FREE SYSTEM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION PROFILING; HUMANS; RECOMBINANT PROTEINS;

EID: 37249021351     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-7-64     Document Type: Article

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