-
1
-
-
19444373996
-
Making the most of affinity tags
-
Waugh, D.S. Making the most of affinity tags. Trends Biotechnol. 23, 316-320 (2005).
-
(2005)
Trends Biotechnol
, vol.23
, pp. 316-320
-
-
Waugh, D.S.1
-
2
-
-
0033589826
-
New fusion protein systems designed to give soluble expression in Escherichia coli
-
Davis, G.D., Elisee, C., Newham, D.M. & Harrison, R.G. New fusion protein systems designed to give soluble expression in Escherichia coli. Biotechnol. Bioeng. 65, 382-388 (1999).
-
(1999)
Biotechnol. Bioeng
, vol.65
, pp. 382-388
-
-
Davis, G.D.1
Elisee, C.2
Newham, D.M.3
Harrison, R.G.4
-
3
-
-
4444378435
-
The solubility and stability of recombinant proteins is increased by their fusion to NusA
-
De Marco, V., Stier, G., Blandin, S. & de Marco, A. The solubility and stability of recombinant proteins is increased by their fusion to NusA. Biochem. Biophys. Res. Comm. 322, 766-771 (2004).
-
(2004)
Biochem. Biophys. Res. Comm
, vol.322
, pp. 766-771
-
-
De Marco, V.1
Stier, G.2
Blandin, S.3
de Marco, A.4
-
4
-
-
4444317237
-
Troubleshooting GST fusion protein expression in E. coli
-
Saluta, M. & Bell, P.A. Troubleshooting GST fusion protein expression in E. coli. Life Sci. News 1, 1-3 (1998).
-
(1998)
Life Sci. News
, vol.1
, pp. 1-3
-
-
Saluta, M.1
Bell, P.A.2
-
5
-
-
0343852701
-
Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: A detoxification enzyme and fusion-protein affinity tag
-
Kaplan, W. et al. Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: A detoxification enzyme and fusion-protein affinity tag. Protein Sci. 6, 399-406 (1997).
-
(1997)
Protein Sci
, vol.6
, pp. 399-406
-
-
Kaplan, W.1
-
6
-
-
0030224856
-
Multiple affinity domains for the detection, purification and immobilization of recombinant proteins
-
Nilsson, J. et al. Multiple affinity domains for the detection, purification and immobilization of recombinant proteins. J. Mol. Recognit. 9, 585-594 (1996).
-
(1996)
J. Mol. Recognit
, vol.9
, pp. 585-594
-
-
Nilsson, J.1
-
7
-
-
0031214792
-
6-tag and maltose-binding protein double-affiritty fusion system
-
6-tag and maltose-binding protein double-affiritty fusion system. Protein Expr. Purif. 10, 309-319 (1997).
-
(1997)
Protein Expr. Purif
, vol.10
, pp. 309-319
-
-
Pryor, K.D.1
Leiting, B.2
-
8
-
-
0032520638
-
A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli
-
Smith, P.A. et al. A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli. Nucleic Acids Res. 26, 1414-1420 (1998).
-
(1998)
Nucleic Acids Res
, vol.26
, pp. 1414-1420
-
-
Smith, P.A.1
-
10
-
-
33645471986
-
A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production
-
Cabrita, L.D., Dai, W. & Bottomley, S.P: A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotechnol. 6, 12 (2006).
-
(2006)
BMC Biotechnol
, vol.6
, pp. 12
-
-
Cabrita, L.D.1
Dai, W.2
Bottomley, S.P.3
-
11
-
-
29244442950
-
Simplified screening for the detection of soluble constructs expressed in E. coli using a modular set of vectors
-
Dümmler, A., Lawrence, A.-M. & de Marco, A. Simplified screening for the detection of soluble constructs expressed in E. coli using a modular set of vectors. Microbial Cell Factories 4, 34 (2006).
-
(2006)
Microbial Cell Factories
, vol.4
, pp. 34
-
-
Dümmler, A.1
Lawrence, A.-M.2
de Marco, A.3
-
12
-
-
0034956228
-
A strategy for optimizing the monodispersity of the fusion proteins: Application to purification of recombinant HPV E6 oncoprotein
-
Nominé, Y. et al. A strategy for optimizing the monodispersity of the fusion proteins: Application to purification of recombinant HPV E6 oncoprotein. Prot. Eng. 14, 297-305 (2001).
-
(2001)
Prot. Eng
, vol.14
, pp. 297-305
-
-
Nominé, Y.1
-
13
-
-
29144469368
-
Native folding of aggregation-prone recombinant proteins in Escherichia coli by osmolytes, plasmid- or benzyl alcohol over-expressed molecular chaperones
-
de Marco, A., Vigh L., Diamant, S. & Goloubinoff, P. Native folding of aggregation-prone recombinant proteins in Escherichia coli by osmolytes, plasmid- or benzyl alcohol over-expressed molecular chaperones. Cell Stress Chaperones 10, 329-339 (2005).
-
(2005)
Cell Stress Chaperones
, vol.10
, pp. 329-339
-
-
de Marco, A.1
Vigh, L.2
Diamant, S.3
Goloubinoff, P.4
-
14
-
-
0035711194
-
Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency
-
Kapust, R.B. et al. Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng. 14, 993-1000 (2001).
-
(2001)
Protein Eng
, vol.14
, pp. 993-1000
-
-
Kapust, R.B.1
|