A New Strategy for the Synthesis of Glycoproteins

Science

Volumn 303, Issue 5656, 2004, Pages 371-373

  Zhang, Zhiwen ^a   Gildersleeve, Jeff ^a   Yang, Yu Ying ^a   Xu, Ran ^a   Loo, Joseph A ^b   Uryu, Sean ^a   Wong, Chi Huey ^a   Schultz, Peter G ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CARBOHYDRATES; CRYSTAL STRUCTURE; ESCHERICHIA COLI; GENES; METABOLISM;

COTRANSLATIONAL SYNTHESIS;

PROTEINS;

BETA N ACETYLGLUCOSAMINE; CARBOHYDRATE; GLUCOSAMINE DERIVATIVE; GLYCOPROTEIN; MYOGLOBIN; SERINE; UNCLASSIFIED DRUG;

PROTEIN;

ACETYLATION; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC CODE; GLYCOPROTEIN SYNTHESIS; METABOLISM; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN GLYCOSYLATION; PROTEIN METHYLATION; PROTEIN PROCESSING; PROTEIN STRUCTURE; REGULATORY MECHANISM; SIGNAL TRANSDUCTION;

ACETYLATION; ACETYLGLUCOSAMINE; CODON; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GLYCOPROTEINS; GLYCOSYLATION; LECTINS; MUTATION; MYOGLOBIN; PROTEIN ENGINEERING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RNA, TRANSFER, TYR; SERINE; SUPPRESSION, GENETIC; TYROSINE-TRNA LIGASE; URIDINE DIPHOSPHATE GALACTOSE;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0345742616     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1089509     Document Type: Article

References (24)

1. R. A. Dwek, Chem. Rev. 96, 683 (1996).
2. C. R. Bertozzi, L. L. Kiessling, Science 291, 2357 (2001).
3. P. Sears, C.-H. Wong, Science 291, 2344 (2001).
4. M. Wacker et al., Science 298, 1790 (2002).
5. B. G. Davis, Chem. Rev. 102, 579 (2002).
6. H. C. Hang, C. R. Bertozzi, Acc. Chem. Res. 34, 727 (2001).
7. L. Wang, A. Brock, B. Herberich, P. G. Schultz, Science 292, 498 (2001).
8. L. Wang, A. Brock, P. G. Schultz, J. Am. Chem. Soc. 124, 1836 (2002).
9. Z. Zhang, L. Wang, A. Brock, P. G. Schultz, Angew. Chem. Int. Ed. Engl. 41, 2840 (2002).
10. J. W. Chin et al., J. Am. Chem. Soc. 124, 9026 (2002).
11. J. W. Chin et al., Proc. Natl. Acad. Sci. U.S.A. 99, 11020 (2002).
12. S. W. Santoro, L. Wang, B. Herberich, D. S. King, P. G. Schultz, Nature Biotechnol. 20, 1044 (2002).
13. L. Wang, Z. Zhang, A. Brock, P. G. Schultz, Proc. Natl. Acad. Sci. U.S.A. 100, 56 (2003).
14. Z. Zhang et al., Biochemistry. 42, 6735 (2003).
15. J. W. Chin et al., Science 301, 964 (2003).
16. T. Arslan, S. V. Mamaev, N. V. Mamaev, S. M. Hecht, J. Am. Chem. Soc. 119, 10877 (1997).
17. L. Wells, K. Vosseller, G. W. Hart, Science 291, 2376 (2001).
18. N. Lamarre-Vincent, L. Hsieh-Wilson, J. Am. Chem. Soc. 125, 6612 (2003).
19. A. K. Sarkar, T. A. Fritz, W. H. Taylor, J. D. Esko, Proc. Natl. Acad. Sci. U.S.A. 92, 3323 (1995).
20. The loss of the N-terminal Met is common in E. coli.
21. S. Ebisu, P. N. S. Iyer, I. J. Goldstein, Carbohydr. Res. 61, 129 (1978).
22. K. Kamemura, B. K. Hayes, F. I. Comer, G. W. Hart, J. Biol. Chem. 277, 19229 (2002).
23. K. Witte, P. Sears, C.-H. Wong, J. Am. Chem. Soc. 119, 2114 (1997).
24. We thank L. Wang, S. Santoro, and A. Martin for providing plasmids for this project. Z.Z. is grateful for a National Research Service Award fellowship (GM66494). J.G. gratefully acknowledges a postdoctoral fellowship from NIH (grant 5 F32 AI10419). Funded by a grant from Department of Energy (DOE, DE-FG03-00ER45812). This is paper 15869-CH of the Scripps Research Institute.