A hybrid plasmid for expression of toxic malarial proteins in Escherichia coli

Molecular and Biochemical Parasitology

Volumn 117, Issue 2, 2001, Pages 245-247

  Cinquin, Olivier ^a   Christopherson, Richard I ^a   Menz, R I ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Over expression; Plasmodium falciparum; pLysS; Rare codon; RIG plasmid; Toxic protein

Indexed keywords

DNA; OROTIDINE 5' PHOSPHATE DECARBOXYLASE; PLASMID VECTOR; PROTOZOAL PROTEIN; RNA POLYMERASE;

ANALYTIC METHOD; ARTICLE; BACTERIAL GROWTH; BODY REGIONS; CELL CULTURE; CELL TRANSFORMATION; CODON; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; GENETIC CODE; NONHUMAN; OPTICAL DENSITY; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY;

ANIMALS; CODON; ESCHERICHIA COLI; GENE DOSAGE; HUMANS; OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; PLASMIDS; PLASMODIUM FALCIPARUM; PROTOZOAN PROTEINS; RNA, TRANSFER;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PLASMODIUM FALCIPARUM;

EID: 0034797685     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(01)00354-1     Document Type: Article

References (11)

1. Codon usage tabulated from the GenBank genetic sequence data
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3. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli
4. Errors of heterologous protein expression
5. Overexpression of an mRNA dependent on rare codons inhibits protein synthesis and cell growth
6. Overcoming codon bias: A method for the high-level overexpression of Plasmodium and other AT-rich parasite genes in Escherichia coli
7. Pyrimidine nucleotide biosynthesis in animals: Genes enzymes and regulation of UMP biosynthesis
8. Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: A model from DNA-binding studies of mutant proteins by surface plasmon resonance
9. Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system
10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4