Rapid and easy thermodynamic optimization of the 5′-end of mRNA dramatically increases the level of wild type protein expression in Escherichia coli

Protein Expression and Purification

Volumn 45, Issue 2, 2006, Pages 374-380

  Cèbe, Régis ^a   Geiser, Martin ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Fusion proteins; Initiation of translation; mRNA secondary structure

Indexed keywords

ANAPHASE PROMOTING COMPLEX; ANAPHASE-PROMOTING COMPLEX; BONE MORPHOGENETIC PROTEIN; MESSENGER RNA; PHOSPHOTRANSFERASE; SOST PROTEIN, HUMAN; SPHINGOSINE KINASE; UBIQUITIN PROTEIN LIGASE;

5' UNTRANSLATED REGION; ARTICLE; CHEMISTRY; CONFORMATION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC MARKER; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; THERMODYNAMICS;

5' UNTRANSLATED REGIONS; BASE SEQUENCE; BONE MORPHOGENETIC PROTEINS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC MARKERS; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); RNA, MESSENGER; THERMODYNAMICS; UBIQUITIN-PROTEIN LIGASE COMPLEXES;

ESCHERICHIA COLI;

EID: 31044446735     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.07.007     Document Type: Article

References (15)

1. K.E. Griswold, N.A. Mahmood, B.L. Iverson, and G. Georgiou Effects of codon usage versus putative 5′-mRNA structure on the expression of Fusarium solani cutinase in the Escherichia coli cytoplasm Protein Expr. Purif. 27 2003 134 142
2. M.H. de Smit, and J. van Duin Translational standby sites: how ribosomes may deal with the rapid folding kinetics of mRNA J. Mol. Biol. 331 2003 737 743
3. M.H. de Smit, and J. van Duin Secondary structure of the ribosome binding site determines translational efficiency: a quantitative analysis Proc. Natl. Acad. Sci. USA 87 1990 7668 7672
4. O.C. Wallis, A.J. Sami, and M. Wallis The effect of changes in nucleotide sequence coding for the N-terminus on expression levels of ovine growth hormone variants in Escherichia coli Biochim. Biophys. Acta 1261 1995 360 368
5. A. Helke, R.M. Geisen, M. Vollmer, M.L. Sprengart, Fuchs Eckart, An unstructured mRNA region and a 5′ hairpin represent elements of the E. coli translation initiation signal determined by using the bacteriophage T7 gene 1 translation start site, Nucleic Acids Res. 21 (1993) 5705-5711.
6. L.H. Tessier, P. Sondermeyer, T. Faure, D. Dreyer, A. Benavente, D. Villeval, M. Courtney, and J. Lecocq The influence of mRNA primary and secondary structure on human IFN-γ gene expression in E. coli Nucleic Acids Res. 12 1984 7663 7675
7. V. Satchidanandam, and Y. Shivashankar Availability of a second upstream AUG can completely overcome inhibition of protein synthesis initiation engendered by mRNA secondary structure encompassing the start codon Gene 196 1997 231 237
8. M. Geiser, R. Cebe, D. Drewello, and R. Schmitz Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase Biotechniques 31 2001 88 92
9. G. Zeng Sticky-end PCR-new method for subcloning Biotechniques 25 1998 206 208
10. M. Zuker, and P. Stiegler Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information Nucleic Acids Res. 9 1981 133 148
11. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
12. M. Hammarstrom, N. Hellgren, S. van den Berg, H. Berglund, and T. Hard Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli Protein Sci. 11 2002 313 321
13. E.I. Gonzalez de Valdivia, and L.A. Isaksson A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli Nucleic Acids Res. 32 2004 5198 5205
14. N. Puri, K.B.C. Appa Rao, S. Menon, A.K. Panda, G. Tiwari, L.C. Garg, and S.M. Totey Effect of the codon following the ATG start site on the expression of ovine growth hormone in Escherichia coli Protein Expr. Purif. 17 1999 215 223
15. M.A. Mukund, T. Bannerjee, I. Ghosh, and S. Datta Effect of mRNA secondary structure in the regulation of gene expression: unfolding of stable loop causes the expression of Taq polymerase in E. coli Curr. Sci. 76 1999 1486 1494