Heterologous high-level E. coli expression, purification and biophysical characterization of the spine-associated RapGAP (SPAR) PDZ domain

Protein Expression and Purification

Volumn 62, Issue 1, 2008, Pages 9-14

  Brown, Breann L ^a   Hadley, Michael ^b   Page, Rebecca ^b  

^a BROWN UNIVERSITY   (United States)

^b BROWN UNIVERSITY   (United States)

Author keywords

Escherichia coli; PDZ domain; Solubility expression tag; Spine associated RapGAP (SPAR)

Indexed keywords

CARRIER PROTEIN; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; MALTOSE BINDING PROTEIN; MALTOSE-BINDING PROTEIN; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; SPA1 PROTEIN, RAT;

ANIMAL; ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; NUCLEAR MAGNETIC RESONANCE; PDZ DOMAIN; PROTEIN FOLDING; RAT;

ANIMALS; CARRIER PROTEINS; ESCHERICHIA COLI; GTPASE-ACTIVATING PROTEINS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PDZ DOMAINS; PROTEIN FOLDING; RATS; RECEPTORS, N-METHYL-D-ASPARTATE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ESCHERICHIA COLI;

EID: 52949117507     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.07.003     Document Type: Article

References (20)

1. Kennedy M.B. Signal-processing machines at the postsynaptic density. Science 290 (2000) 750-754
2. Dunaevsky A., Blazeski R., Yuste R., and Mason C. Spine motility with synaptic contact. Nat. Neurosci. 4 (2001) 685-686
3. Fischer M., Kaech S., Knutti D., and Matus A. Rapid actin-based plasticity in dendritic spines. Neuron 20 (1998) 847-854
4. Irwin S.A., Galvez R., and Greenough W.T. Dendritic spine structural anomalies in fragile-X mental retardation syndrome. Cereb. Cortex 10 (2000) 1038-1044
5. Kaufmann W.E., and Moser H.W. Dendritic anomalies in disorders associated with mental retardation. Cereb. Cortex 10 (2000) 981-991
6. Robinson T.E., and Kolb B. Alterations in the morphology of dendrites and dendritic spines in the nucleus accumbens and prefrontal cortex following repeated treatment with amphetamine or cocaine. Eur. J. Neurosci. 11 (1999) 1598-1604
7. Rosoklija G., Toomayan G., Ellis S.P., Keilp J., Mann J.J., Latov N., Hays A.P., and Dwork A.J. Structural abnormalities of subicular dendrites in subjects with schizophrenia and mood disorders: preliminary findings. Arch. Gen. Psychiatry 57 (2000) 349-356
8. Pak D.T., and Sheng M. Targeted protein degradation and synapse remodeling by an inducible protein kinase. Science 302 (2003) 1368-1373
9. Pak D.T., Yang S., Rudolph-Correia S., Kim E., and Sheng M. Regulation of dendritic spine morphology by SPAR, a PSD-95-associated RapGAP. Neuron 31 (2001) 289-303
10. Meyer G., Varoqueaux F., Neeb A., Oschlies M., and Brose N. The complexity of PDZ domain-mediated interactions at glutamatergic synapses: a case study on neuroligin. Neuropharmacology 47 (2004) 724-733
11. Peti W., and Page R. Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Protein Expr. Purif. 51 (2007) 1-10
12. Kapust R.B., and Waugh D.S. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 8 (1999) 1668-1674
13. Nallamsetty S., and Waugh D.S. Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners. Protein Expr. Purif. 45 (2006) 175-182
14. Waugh D.S. Making the most of affinity tags. Trends Biotechnol. 23 (2005) 316-320
15. Kapust R.B., Tozser J., Fox J.D., Anderson D.E., Cherry S., Copeland T.D., and Waugh D.S. Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng. 14 (2001) 993-1000
16. Kapust R.B., and Waugh D.S. Controlled intracellular processing of fusion proteins by TEV protease. Protein Expr. Purif. 19 (2000) 312-318
17. Jaroszewski L., Rychlewski L., Li Z., Li W., and Godzik A. FFAS03: a server for profile--profile sequence alignments. Nucleic Acids Res. 33 (2005) W284-288
18. Graslund S., Eklund M., Falk R., Uhlen M., Nygren P.A., and Stahl S. A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products. J. Biotechnol. 99 (2002) 41-50
19. Dummler A., Lawrence A.M., and de Marco A. Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors. Microb. Cell. Fact. 4 (2005) 34
20. Page R., Peti W., Wilson I.A., Stevens R.C., and Würthrich K. NMR screening and crystal quality of bacterial expressed prokaryotic and eukaryotic proteins in a structural genomics pipeline. Proc. Natl. Acad. Sci. USA 102 (2005) 1901-1905