Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts

Proteins: Structure, Function and Genetics

Volumn 71, Issue 2, 2008, Pages 982-994

  Klock, Heath E ^a   Koesema, Eric J ^a   Knuth, Mark W ^a   Lesley, Scott A ^a,b  

^a GENOMICS INSTITUTE OF THE NOVARTIS RESEARCH FOUNDATION   (United States)

^b NONE   (United States)

Author keywords

Construct optimization; High throughput; Ligase independent; PCR; Sequence independent; Site directed

Indexed keywords

ARTICLE; CLONING; GEL PERMEATION CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS; SOLUBILITY; STRUCTURAL GENOMICS; X RAY CRYSTALLOGRAPHY;

CLONING, MOLECULAR; CRYSTALLIZATION; DNA PRIMERS; GENOMICS; MUTAGENESIS; POLYMERASE CHAIN REACTION;

EID: 41149148236     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.21786     Document Type: Article

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