Current status of polymeric gene delivery systems

Advanced Drug Delivery Reviews

Volumn 58, Issue 4, 2006, Pages 467-486

  Park, Tae Gwan ^a   Jeong, Ji Hoon ^b   Kim, Sung Wan ^b  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b UNIVERSITY OF UTAH   (United States)

Author keywords

Cationic polymers; Gene therapy; Neutral polymers; Non viral gene delivery; Tissue engineering

Indexed keywords

BIOLOGICAL MEMBRANES; CONTROLLED DRUG DELIVERY; DISEASE CONTROL; GENE TRANSFER; IMMUNOLOGY; PATIENT TREATMENT; PROTEINS; TOXICITY;

GENE THERAPY; NEUTRAL POLYMERS; NON-VIRAL GENE DELIVERYCATIONIC POLYMERS; TISSUE ENGINEERING;

GENES;

ANTIBODY; ARGINYLGLYCYLASPARTIC ACID; ARTERIAL WALL BINDING PROTEIN; CARRIER PROTEIN; CHITOSAN; COMPLEMENTARY RNA; COPOLYMER; CYCLODEXTRIN; DRUG CARRIER; FOLIC ACID; GALACTOSE; IMMUNOGLOBULIN FC FRAGMENT; LACTOSE; LOW DENSITY LIPOPROTEIN; MACROGOL; PLASMA PROTEIN; PLASMID DNA; POLY(2 AMINOETHYLPROPYLENE PHOSPHATE); POLY(BETA AMINO ESTER); POLY[2 (DIMETHYLAMINO)ETHYL METHACRYLATE]; POLY[ALPHA(4 AMINOBUTYL) 1 GLYCOLIC ACID]; POLYCATION; POLYELECTROLYTE; POLYETHYLENEIMINE; POLYLYSINE; POLYSACCHARIDE; UNCLASSIFIED DRUG; UNINDEXED DRUG; VASCULOTROPIN; VASCULOTROPIN RECEPTOR; VASCULOTROPIN RECEPTOR 1; VIRUS VECTOR;

BINDING AFFINITY; BIODEGRADABILITY; CELL MEMBRANE; CELL NUCLEUS MEMBRANE; CHEMICAL MODIFICATION; CHEMICAL STRUCTURE; COMPLEX FORMATION; CONJUGATION; CYTOTOXICITY; GENE TARGETING; GENE TRANSFER; GENE VECTOR; GENETIC TRANSFECTION; GOOD MANUFACTURING PRACTICE; HUMAN; HYDROPHILICITY; HYDROPHOBICITY; IMMUNOGENICITY; MICELLE; MOLECULAR WEIGHT; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; REVIEW; SURFACE PROPERTY; TISSUE SPECIFICITY;

ANIMALS; GENE THERAPY; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; MOLECULAR STRUCTURE; POLYETHYLENEIMINE; POLYLYSINE; POLYMERS;

EID: 33745460260     PISSN: 0169409X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.addr.2006.03.007     Document Type: Review

References (134)

1. Ledley F.D. Pharmaceutical approach to somatic gene therapy. Pharm. Res. 13 (1996) 1595-1614
2. Vacik J., Dean B.S., Zimmer W.E., and Dean D.A. Cell-specific nuclear import of plasmid DNA. Gene Ther. 6 (1999) 1006-1014
3. Schaffer D.V., and Lauffenburger D.A. Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery. J. Biol. Chem. 273 (1998) 28004-28009
4. Breyer B., Jiang W., Cheng H., Zhou L., Paul R., Feng T., and He T.C. Adenoviral vector-mediated gene transfer for human gene therapy. Curr. Gene Ther. 1 (2001) 149-162
5. Han S., Mahato R.I., Sung Y.K., and Kim S.W. Development of biomaterials for gene therapy. Molec. Ther. 2 (2000) 302-317
6. Lee M., and Kim S.W. Polyethylene glycol-conjugated copolymers for plasmid DNA delivery. Pharm. Res. 22 (2005) 1-10
7. Glover D.J., Lipps H.J., and Jans D.A. Towards safe, non-viral therapeutic gene expression in humans. Nat. Rev., Genet. 6 (2005) 299-310
8. Laemmli U.K. Characterization of DNA condensates induced by poly(ethylene oxide) and polylysine. Proc. Natl. Acad. Sci. U. S. A. 72 (1975) 4288-4292
9. Kwoh D.Y., Coffin C.C., Lollo C.P., Jovenal J., Banaszczyk M.G., Mullen P., Phillips A., Amini A., Fabrycki J., Bartholomew R.M., Brostoff S.W., and Carlo D.J. Stabilization of poly-l-lysine/DNA polyplexes for in vivo gene delivery to the liver. Biochim. Biophys. Acta 1444 (1999) 171-190
10. Choi Y.H., Liu F., Kim J.S., Choi Y.K., Park J.S., and Kim S.W. Polyethylene glycol-grafted poly-l-lysine as polymeric gene carrier. J. Control. Release 54 (1998) 39-48
11. Liu G., Molas M., Grossmann G.A., Pasumarthy M., Perales J.C., Cooper M.J., and Hanson R.W. Biological properties of poly-l-lysine-DNA complexes generated by cooperative binding of the polycation. J. Biol. Chem. 276 (2001) 34379-34387
12. Ogris M., Brunner S., Schuller S., Kircheis R., and Wagner E. PEGylated DNA/transferrin-PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery. Gene Ther. 6 (1999) 595-605
13. Harada-Shiba M., Yamauchi K., Harada A., Takamisawa I., Shimokado K., and Kataoka K. Polyion complex micelles as vectors in gene therapy-pharmacokinetics and in vivo gene transfer. Gene Ther. 9 (2002) 407-414
14. Wolfert M.A., Schacht E.H., Toncheva V., Ulbrich K., Nazarova O., and Seymour L.W. Characterization of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers. Hum. Gene Ther. 7 (1996) 2123-2133
15. Katayose S., and Kataoka K. Water-soluble polyion complex associates of DNA and poly(ethylene glycol)-poly(l-lysine) block copolymer. Bioconjug. Chem. 8 (1997) 702-707
16. Kataoka K. Intracellular gene delivery by polymer micelle vectors. Nippon Rinsho 56 (1998) 718-723
17. Kakizawa Y., Harada A., and Kataoka K. Glutathione-sensitive stabilization of block copolymer micelles composed of antisense DNA and thiolated poly(ethylene glycol)-block-poly(l-lysine): a potential carrier for systemic delivery of antisense DNA. Biomacromolecules 2 (2001) 491-497
18. Harada A., Togawa H., and Kataoka K. Physicochemical properties and nuclease resistance of antisense-oligodeoxynucleotides entrapped in the core of polyion complex micelles composed of poly(ethylene glycol)-poly(l-lysine) block copolymers. Eur. J. Pharm. Sci. 13 (2001) 35-42
19. Lee M., Han S.O., Ko K.S., Koh J.J., Park J.S., Yoon J.W., and Kim S.W. Repression of GAD autoantigen expression in pancreas beta-cells by delivery of antisense plasmid/PEG-g-PLL complex. Molec. Ther. 4 (2001) 339-346
20. Midoux P., Mendes C., Legrand A., Raimond J., Mayer R., Monsigny M., and Roche A.C. Specific gene transfer mediated by lactosylated poly-l-lysine into hepatoma cells. Nucleic Acids Res. 21 (1993) 871-878
21. Nishikawa M., Takemura S., Takakura Y., and Hashida M. Targeted delivery of plasmid DNA to hepatocytes in vivo: optimization of the pharmacokinetics of plasmid DNA/galactosylated poly(l-lysine) complexes by controlling their physicochemical properties. J. Pharmacol. Exp. Ther. 287 (1998) 408-415
22. Hashida M., Takemura S., Nishikawa M., and Takakura Y. Targeted delivery of plasmid DNA complexed with galactosylated poly(l-lysine). J. Control. Release 53 (1998) 301-310
23. Mahato R.I., Takemura S., Akamatsu K., Nishikawa M., Takakura Y., and Hashida M. Physicochemical and disposition characteristics of antisense oligonucleotides complexed with glycosylated poly(l-lysine). Biochem. Pharmacol. 53 (1997) 887-895
24. Choi Y.H., Liu F., Park J.S., and Kim S.W. Lactose-poly(ethylene glycol)-grafted poly-l-lysine as hepatoma cell-targeted gene carrier. Bioconjug. Chem. 9 (1998) 708-718
25. Choi Y.H., Liu F., Choi J.S., Kim S.W., and Park J.S. Characterization of a targeted gene carrier, lactose-polyethylene glycol-grafted poly-l-lysine and its complex with plasmid DNA. Hum. Gene Ther. 10 (1999) 2657-2665
26. Shih I.L., Lees R.S., Chang M.Y., and Lees A.M. Focal accumulation of an apolipoprotein B-based synthetic oligopeptide in the healing rabbit arterial wall. Proc. Natl. Acad. Sci. U. S. A. 87 (1990) 1436-1440
27. Nah J.W., Yu L., Han S.O., Ahn C.H., and Kim S.W. Artery wall binding peptide-poly(ethylene glycol)-grafted-poly(l-lysine)-based gene delivery to artery wall cells. J. Control. Release 78 (2002) 273-284
28. Suh W., Chung J.K., Park S.H., and Kim S.W. Anti-JL1 antibody-conjugated poly(l-lysine) for targeted gene delivery to leukemia T cells. J. Control. Release 72 (2001) 171-178
29. Trubetskoy V.S., Torchilin V.P., Kennel S.J., and Huang L. Use of N-terminal modified poly(l-lysine)-antibody conjugate as a carrier for targeted gene delivery in mouse lung endothelial cells. Bioconjug. Chem. 3 (1992) 323-327
30. Lu Y., and Low P.S. Folate-mediated delivery of macromolecular anticancer therapeutic agents. Adv. Drug Deliv. Rev. 54 (2002) 675-693
31. Mislick K.A., Baldeschwieler J.D., Kayyem J.F., and Meade T.J. Transfection of folate-polylysine DNA complexes: evidence for lysosomal delivery. Bioconjug. Chem. 6 (1995) 512-515
32. Cho K.C., Kim S.H., Jeong J.H., and Park T.G. Folate receptor-mediated gene delivery using folate-poly(ethylene glycol)-poly(l-lysine) conjugate. Macromol. Biosci. 5 (2005) 512-519
33. Kim J.S., Kim B.I., Maruyama A., Akaike T., and Kim S.W. A new non-viral DNA delivery vector: the Terplex system. J. Control. Release 53 (1998) 175-182
34. Kim J.S., Maruyama A., Akaike T., and Kim S.W. Terplex DNA delivery system as a gene carrier. Pharm. Res. 15 (1998) 116-121
35. Havel R.J. Receptor and non-receptor mediated uptake of chylomicron remnants by the liver. Atherosclerosis 1 (1998) S1-S7
36. Sawamura T., Kume N., Aoyama T., Moriwaki H., Hoshikawa H., Aiba Y., Tanaka T., Miwa S., Katsura Y., Kita T., and Masaki T. An endothelial receptor for oxidized low-density lipoprotein. Nature 386 (1997) 73-77
37. Hein M., Ernst M., Moller F., and Regensburger D. Gene transfer into rat heart-derived endothelial cells. Eur. J. Cardio-Thorac. Surg. 13 (1998) 460-466
38. Yu L., Suh H., Koh J.J., and Kim S.W. Systemic administration of TerplexDNA system: pharmacokinetics and gene expression. Pharm. Res. 18 (2001) 1277-1283
39. Affleck D.G., Yu L., Bull D.A., Bailey S.H., and Kim S.W. Augmentation of myocardial transfection using TerplexDNA: a novel gene delivery system. Gene Ther. 8 (2001) 349-353
40. Bull D.A., Bailey S.H., Rentz J.J., Zebrack J.S., Lee M., Litwin S.E., and Kim S.W. Effect of Terplex/VEGF-165 gene therapy on left ventricular function and structure following myocardial infarction. VEGF gene therapy for myocardial infarction. J. Control. Release 93 (2003) 175-181
41. Garnett M.C. Gene-delivery systems using cationic polymers. Crit. Rev. Ther. Drug Carr. Syst. 16 (1999) 147-207
42. Boussif O., Lezoualc'h F., Zanta M.A., Mergny M.D., Scherman D., Demeneix B., and Behr J.P. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc. Natl. Acad. Sci. U. S. A. 92 (1995) 7297-7301
43. Jeong J.H., Song S.H., Lim D.W., Lee H., and Park T.G. DNA transfection using linear poly(ethylenimine) prepared by controlled acid hydrolysis of poly(2-ethyl-2-oxazoline). J. Control. Release 73 (2001) 391-399
44. Ferrari S., Moro E., Pettenazzo A., Behr J.P., Zacchello F., and Scarpa M. ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo. Gene Ther. 4 (1997) 1100-1106
45. Coll J.L., Chollet P., Brambilla E., Desplanques D., Behr J.P., and Favrot M. In vivo delivery to tumors of DNA complexed with linear polyethylenimine. Hum. Gene Ther. 10 (1999) 1659-1666
46. Wightman L., Kircheis R., Rossler V., Carotta S., Ruzicka R., Kursa M., and Wagner E. Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo. J. Gene Med. 3 (2001) 362-372
47. Petersen H., Fechner P.M., Martin A.L., Kunath K., Stolnik S., Roberts C.J., Fischer D., Davies M.C., and Kissel T. Polyethylenimine-graft-poly(ethylene glycol) copolymers: influence of copolymer block structure on DNA complexation and biological activities as gene delivery system. Bioconjug. Chem. 13 (2002) 845-854
48. Lee H., Jeong J.H., and Park T.G. A new gene delivery formulation of polyethylenimine/DNA complexes coated with PEG conjugated fusogenic peptide. J. Control. Release 76 (2001) 183-192
49. Mishra S., Webster P., and Davis M.E. PEGylation significantly affects cellular uptake and intracellular trafficking of non-viral gene delivery particles. Eur. J. Cell Biol. 83 (2004) 97-111
50. Shi L., Tang G.P., Gao S.J., Ma Y.X., Liu B.H., Li Y., Zeng J.M., Ng Y.K., Leong K.W., and Wang S. Repeated intrathecal administration of plasmid DNA complexed with polyethylene glycol-grafted polyethylenimine led to prolonged transgene expression in the spinal cord. Gene Ther. 10 (2003) 1179-1188
51. Suh W., Han S.O., Yu L., and Kim S.W. An angiogenic, endothelial-cell-targeted polymeric gene carrier. Molec. Ther. 6 (2002) 664-672
52. Kim W.J., Yockman J.W., Lee M., Jeong J.H., Kim Y.H., and Kim S.W. Soluble Flt-1 gene delivery using PEI-g-PEG-RGD conjugate for anti-angiogenesis. J. Control. Release 106 (2005) 224-234
53. Sipkins D.A., Cheresh D.A., Kazemi M.R., Nevin L.M., Bednarski M.D., and Li K.C. Detection of tumor angiogenesis in vivo by alphaVbeta3-targeted magnetic resonance imaging. Nat. Med. 4 (1998) 623-626
54. Gasparini G., Brooks P.C., Biganzoli E., Vermeulen P.B., Bonoldi E., Dirix L.Y., Ranieri G., Miceli R., and Cheresh D.A. Vascular integrin alpha(v)beta3: a new prognostic indicator in breast cancer. Clin. Cancer Res. 4 (1998) 2625-2634
55. Erdreich-Epstein A., Shimada H., Groshen S., Liu M., Metelitsa L.S., Kim K.S., Stins M.F., Seeger R.C., and Durden D.L. Integrins alpha(v)beta3 and alpha(v)beta5 are expressed by endothelium of high-risk neuroblastoma and their inhibition is associated with increased endogenous ceramide. Cancer Res. 60 (2000) 712-721
56. Ruoslahti E. RGD and other recognition sequences for integrins. Annu. Rev. Cell Dev. Biol. 12 (1996) 697-715
57. Kunath K., Merdan T., Hegener O., Haberlein H., and Kissel T. Integrin targeting using RGD-PEI conjugates for in vitro gene transfer. J. Gene Med. 5 (2003) 588-599
58. Chiu S.J., Ueno N.T., and Lee R.J. Tumor-targeted gene delivery via anti-HER2 antibody (trastuzumab, Herceptin) conjugated polyethylenimine. J. Control. Release 97 (2004) 357-369
59. Merdan T., Callahan J., Petersen H., Kunath K., Bakowsky U., Kopeckova P., Kissel T., and Kopecek J. Pegylated polyethylenimine-Fab′ antibody fragment conjugates for targeted gene delivery to human ovarian carcinoma cells. Bioconjug. Chem. 14 (2003) 989-996
60. Jeong J.H., Lee M., Kim W.J., Yockman J.W., Park T.G., Kim Y.H., and Kim S.W. Anti-GAD antibody targeted non-viral gene delivery to islet beta cells. J. Control. Release 107 (2005) 562-570
61. Benns J.M., Maheshwari A., Furgeson D.Y., Mahato R.I., and Kim S.W. Folate-PEG-folate-graft-polyethylenimine-based gene delivery. J. Drug Target. 9 (2001) 123-139
62. Kim S.H., Jeong J.H., Cho K.C., Kim S.W., and Park T.G. Target-specific gene silencing by siRNA plasmid DNA complexed with folate-modified poly(ethylenimine). J. Control. Release 104 (2005) 223-232
63. Han S., Mahato R.I., and Kim S.W. Water-soluble lipopolymer for gene delivery. Bioconjug. Chem. 12 (2001) 337-345
64. Kunath K., von Harpe A., Fischer D., Petersen H., Bickel U., Voigt K., and Kissel T. Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine. J. Control. Release 89 (2003) 113-125
65. Godbey W.T., Wu K.K., and Mikos A.G. Size matters: molecular weight affects the efficiency of poly(ethylenimine) as a gene delivery vehicle. J. Biomed. Mater. Res. 45 (1999) 268-275
66. Varga C.M., Tedford N.C., Thomas M., Klibanov A.M., Griffith L.G., and Lauffenburger D.A. Quantitative comparison of polyethylenimine formulations and adenoviral vectors in terms of intracellular gene delivery processes. Gene Ther. 12 (2005) 1023-1032
67. Mahato R.I., Lee M., Han S., Maheshwari A., and Kim S.W. Intratumoral delivery of p2CMVmIL-12 using water-soluble lipopolymers. Molec. Ther. 4 (2001) 130-138
68. Yockman J.W., Maheshwari A., Han S.O., and Kim S.W. Tumor regression by repeated intratumoral delivery of water soluble lipopolymers/p2CMVmIL-12 complexes. J. Control. Release 87 (2003) 177-186
69. Lee M., Rentz J., Han S.O., Bull D.A., and Kim S.W. Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium. Gene Ther. 10 (2003) 585-593
70. Lee M., Rentz J., Bikram M., Han S., Bull D.A., and Kim S.W. Hypoxia-inducible VEGF gene delivery to ischemic myocardium using water-soluble lipopolymer. Gene Ther. 10 (2003) 1535-1542
71. Janat-Amsbury M.M., Yockman J.W., Lee M., Kern S., Furgeson D.Y., Bikram M., and Kim S.W. Combination of local, nonviral IL12 gene therapy and systemic paclitaxel treatment in a metastatic breast cancer model. Molec. Ther. 9 (2004) 829-836
72. Janat-Amsbury M.M., Yockman J.W., Lee M., Kern S., Furgeson D.Y., Bikram M., and Kim S.W. Local, non-viral IL-12 gene therapy using a water soluble lipopolymer as carrier system combined with systemic paclitaxel for cancer treatment. J. Control. Release 101 (2005) 273-285
73. Lim Y.B., Kim C.H., Kim K., Kim S.W., and Park J.S. Developement of a safe gene delivery system using biodegradable polymer, poly[alpha-(4-aminobutyl)-l-glycolic acid]. J. Am. Chem. Soc. 122 (2000) 6524-6525
74. Lim Y.B., Han S.O., Kong H.U., Lee Y., Park J.S., Jeong B., and Kim S.W. Biodegradable polyester, poly[alpha-(4-aminobutyl)-l-glycolic acid], as a non-toxic gene carrier. Pharm. Res. 17 (2000) 811-816
75. Koh J.J., Ko K.S., Lee M., Han S., Park J.S., and Kim S.W. Degradable polymeric carrier for the delivery of IL-10 plasmid DNA to prevent autoimmune insulitis of NOD mice. Gene Ther. 7 (2000) 2099-2104
76. Ko K.S., Lee M., Koh J.J., and Kim S.W. Combined administration of plasmids encoding IL-4 and IL-10 prevents the development of autoimmune diabetes in nonobese diabetic mice. Molec. Ther. 4 (2001) 313-316
77. Maheshwari A., Han S., Mahato R.I., and Kim S.W. Biodegradable polymer-based interleukin-12 gene delivery: role of induced cytokines, tumor infiltrating cells and nitric oxide in anti-tumor activity. Gene Ther. 9 (2002) 1075-1084
78. Lynn D.M., and Langer R. Degradable poly(beta-amino esters): synthesis, characterization, and self-assembly with plasmid DNA. J. Am. Chem. Soc. 122 (2000) 10761-10768
79. Lynn D.M., Anderson D.G., Putnam D., and Langer R. Accelerated discovery of synthetic transfection vectors: parallel synthesis and screening of degradable polymer library. J. Am. Chem. Soc. 123 (2001) 8155-8156
80. Wang J., Mao H.Q., and Leong K.W. A novel biodegradable gene carrier based on polyphosphoester. J. Am. Chem. Soc. 123 (2001) 9480-9481
81. Ahn C.H., Chae S.Y., Bae Y.H., and Kim S.W. Biodegradable poly(ethylenimine) for plasmid DNA delivery. J. Control. Release 80 (2002) 273-282
82. Gosselin M.A., Guo W., and Lee R.J. Efficient gene transfer using reversibly cross-linked low molecular weight polyethylenimine. Bioconjug. Chem. 12 (2001) 989-994
83. Kim Y.H., Park J.H., Lee M., Park T.G., and Kim S.W. Polyethylenimine with acid-labile linkages as a biodegradable gene carrier. J. Control. Release 103 (2005) 209-219
84. Gonzalez H., Hwang S.J., and Davis M.E. New class of polymers for the delivery of macromolecular therapeutics. Bioconjug. Chem. 10 (1999) 1068-1074
85. Hwang S.J., Bellocq N.C., and Davis M.E. Effects of structure of beta-cyclodextrin-containing polymers on gene delivery. Bioconjug. Chem. 12 (2001) 280-290
86. Pun S.H., Bellocq N.C., Liu A., Jensen G., Machemer T., Quijano E., Schluep T., Wen S., Engler H., Heidel J., and Davis M.E. Cyclodextrin-modified polyethylenimine polymers for gene delivery. Bioconjug. Chem. 15 (2004) 831-840
87. Chandy T., and Sharma C.P. Chitosan-as a biomaterial. Biomater. Artif. Cells Artif. Organs 18 (1990) 1-24
88. Schipper N.G., Varum K.M., and Artursson P. Chitosans as absorption enhancers for poorly absorbable drugs: 1. Influence of molecular weight and degree of acetylation on drug transport across human intestinal epithelial (Caco-2) cells. Pharm. Res. 13 (1996) 1686-1692
89. Lee M., Nah J.W., Kwon Y., Koh J.J., Ko K.S., and Kim S.W. Water-soluble and low molecular weight chitosan-based plasmid DNA delivery. Pharm. Res. 18 (2001) 427-431
90. Lee K.Y., Kwon I.C., Kim Y.H., Jo W.H., and Jeong S.Y. Preparation of chitosan self-aggregates as a gene delivery system. J. Control. Release 51 (1998) 213-220
91. Erbacher P., Zou S., Bettinger T., Steffan A.M., and Remy J.S. Chitosan-based vector/DNA complexes for gene delivery: biophysical characteristics and transfection ability. Pharm. Res. 15 (1998) 1332-1339
92. Koping-Hoggard M., Tubulekas I., Guan H., Edwards K., Nilsson M., Varum K.M., and Artursson P. Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo. Gene Ther. 8 (2001) 1108-1121
93. van de Wetering P., Cherng J.Y., Talsma H., Crommelin D.J., and Hennink W.E. 2-(Dimethylamino)ethyl methacrylate based (co)polymers as gene transfer agents. J. Control. Release 53 (1998) 145-153
94. van de Wetering P., Schuurmans-Nieuwenbroek N.M., van Steenbergen M.J., Crommelin D.J., and Hennink W.E. Copolymers of 2-(dimethylamino)ethyl methacrylate with ethoxytriethylene glycol methacrylate or N-vinyl-pyrrolidone as gene transfer agents. J. Control. Release 64 (2000) 193-203
95. Zuidam N.J., Posthuma G., de Vries E.T., Crommelin D.J., Hennink W.E., and Storm G. Effects of physicochemical characteristics of poly(2-(dimethylamino)ethyl methacrylate)-based polyplexes on cellular association and internalization. J. Drug Target. 8 (2000) 51-66
96. Arigita C., Zuidam N.J., Crommelin D.J., and Hennink W.E. Association and dissociation characteristics of polymer/DNA complexes used for gene delivery. Pharm. Res. 16 (1999) 1534-1541
97. Simons M., Edelman E.R., DeKeyser J.L., Langer R., and Rosenberg R.D. Antisense c-myb oligonucleotides inhibit intimal arterial smooth muscle cell accumulation in vivo. Nature 359 (1992) 67-70
98. Stein C.A., and Cheng Y.C. Antisense oligonucleotides as therapeutic agents-is the bullet really magical?. Science 261 (1993) 1004-1012
99. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
100. Elbashir S.M., Lendeckel W., and Tuschl T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15 (2001) 188-200
101. Wagner R.W. Gene inhibition using antisense oligodeoxynucleotides. Nature 372 (1994) 333-335
102. Jeong J.H., and Park T.G. Novel polymer-DNA hybrid polymeric micelles composed of hydrophobic poly(d,l-lactic-co-glycolic acid) and hydrophilic oligonucleotides. Bioconjug. Chem. 12 (2001) 917-923
103. Braasch D.A., Paroo Z., Constantinescu A., Ren G., Oz O.K., Mason R.P., and Corey D.R. Biodistribution of phosphodiester and phosphorothioate siRNA. Bioorg. Med. Chem. Lett. 14 (2004) 1139-1143
104. Jeong J.H., Kim S.W., and Park T.G. Novel intracellular delivery system of antisense oligonucleotide by self-assembled hybrid micelles composed of DNA/PEG conjugate and cationic fusogenic peptide. Bioconjug. Chem. 14 (2003) 473-479
105. Jeong J.H., Kim S.H., Kim S.W., and Park T.G. Polyelectrolyte complex micelles composed of c-raf anti-sense oligodeoxynucleotide-poly(ethylene glycol) conjugate and poly(ethylenimine): effect of systemic administration on tumor growth. Bioconjug. Chem. 16 (2005) 1034-1037
106. Oishi M., Sasaki S., Nagasaki Y., and Kataoka K. pH-responsive oligodeoxynucleotide (ODN)-poly(ethylene glycol) conjugate through acid-labile beta-thiopropionate linkage: preparation and polyion complex micelle formation. Biomacromolecules 4 (2003) 1426-1432
107. Jeong J.H., Kim S.W., and Park T.G. A new antisense oligonucleotide delivery system based on self-assembled ODN-PEG hybrid conjugate micelles. J. Control. Release 93 (2003) 183-191
108. Maeda H., Wu J., Sawa T., Matsumura Y., and Hori K. Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review. J. Control. Release 65 (2000) 271-284
109. Oishi M., Nagasaki Y., Itaka K., Nishiyama N., and Kataoka K. Lactosylated poly(ethylene glycol)-siRNA conjugate through acid-labile beta-thiopropionate linkage to construct pH-sensitive polyion complex micelles achieving enhanced gene silencing in hepatoma cells. J. Am. Chem. Soc. 127 (2005) 1624-1625
110. Rolland A.P., and Mumper R.J. Plasmid delivery to muscle: recent advances in polymer delivery systems. Adv. Drug Deliv. Rev. 30 (1998) 151-172
111. Mumper R.J., Duguid J.G., Anwer K., Barron M.K., Nitta H., and Rolland A.P. Polyvinyl derivatives as novel interactive polymers for controlled gene delivery to muscle. Pharm. Res. 13 (1996) 701-709
112. Mumper R.J., Wang J., Klakamp S.L., Nitta H., Anwer K., Tagliaferri F., and Rolland A.P. Protective interactive noncondensing (PINC) polymers for enhanced plasmid distribution and expression in rat skeletal muscle. J. Control. Release 52 (1998) 191-203
113. Astafieva I., Maksimova I., Lukanidin E., Alakhov V., and Kabanov A. Enhancement of the polycation-mediated DNA uptake and cell transfection with Pluronic P85 block copolymer. FEBS Lett. 389 (1996) 278-280
114. Jeong J.H., Kim S.W., and Park T.G. Biodegradable triblock copolymer of PLGA-PEG-PLGA enhances gene transfection efficiency. Pharm. Res. 21 (2004) 50-54
115. Nam Y.S., and Park T.G. Biodegradable polymeric microcellular foams by modified thermally induced phase separation method. Biomaterials 20 (1999) 1783-1790
116. Nam Y.S., and Park T.G. Porous biodegradable polymeric scaffolds prepared by thermally induced phase separation. J. Biomed. Mater. Res. 47 (1999) 8-17
117. Yoon J.J., Kim J.H., and Park T.G. Dexamethasone-releasing biodegradable polymer scaffolds fabricated by a gas-foaming/salt-leaching method. Biomaterials 24 (2003) 2323-2329
118. Mooney D.J., Baldwin D.F., Suh N.P., Vacanti J.P., and Langer R. Novel approach to fabricate porous sponges of poly(d,l-lactic-co-glycolic acid) without the use of organic solvents. Biomaterials 17 (1996) 1417-1422
119. Hile D.D., Amirpour M.L., Akgerman A., and Pishko M.V. Active growth factor delivery from poly(d,l-lactide-co-glycolide) foams prepared in supercritical CO(2). J. Control. Release 66 (2000) 177-185
120. Fu K., Klibanov A.M., and Langer R. Protein stability in controlled-release systems. Nat. Biotechnol. 18 (2000) 24-25
121. Chun K.W., Cho K.C., Kim S.H., Jeong J.H., and Park T.G. Controlled release of plasmid DNA from biodegradable scaffolds fabricated using a thermally-induced phase-separation method. J. Biomater. Sci., Polym. Ed. 15 (2004) 1341-1353
122. Shea L.D., Smiley E., Bonadio J., and Mooney D.J. DNA delivery from polymer matrices for tissue engineering. Nat. Biotechnol. 17 (1999) 551-554
123. Bonadio J., Smiley E., Patil P., and Goldstein S. Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration. Nat. Med. 5 (1999) 753-759
124. Lee H., Jeong J.H., and Park T.G. PEG grafted polylysine with fusogenic peptide for gene delivery: high transfection efficiency with low cytotoxicity. J. Control. Release 79 (2002) 283-291
125. Cho Y.W., Kim J.D., and Park K. Polycation gene delivery systems: escape from endosomes to cytosol. J. Pharm. Pharmacol. 55 (2003) 721-734
126. Tachibana R., Harashima H., Shinohara Y., and Kiwada H. Quantitative studies on the nuclear transport of plasmid DNA and gene expression employing nonviral vectors. Adv. Drug Deliv. Rev. 52 (2001) 219-226
127. Cartier R., and Reszka R. Utilization of synthetic peptides containing nuclear localization signals for nonviral gene transfer systems. Gene Ther. 9 (2002) 157-167
128. McLachlan G., Stevenson B.J., Davidson D.J., and Porteous D.J. Bacterial DNA is implicated in the inflammatory response to delivery of DNA/DOTAP to mouse lungs. Gene Ther. 7 (2000) 384-392
129. Yew N.S., Zhao H., Wu I.H., Song A., Tousignant J.D., Przybylska M., and Cheng S.H. Reduced inflammatory response to plasmid DNA vectors by elimination and inhibition of immunostimulatory CpG motifs. Molec. Ther. 1 (2000) 255-262
130. Bigger B.W., Tolmachov O., Collombet J.M., Fragkos M., Palaszewski I., and Coutelle C. An araC-controlled bacterial cre expression system to produce DNA minicircle vectors for nuclear and mitochondrial gene therapy. J. Biol. Chem. 276 (2001) 23018-23027
131. Jeong B., Bae Y.H., Lee D.S., and Kim S.W. Biodegradable block copolymers as injectable drug-delivery systems. Nature 388 (1997) 860-862
132. Zentner G.M., Rathi R., Shih C., McRea J.C., Seo M.H., Oh H., Rhee B.G., Mestecky J., Moldoveanu Z., Morgan M., and Weitman S. Biodegradable block copolymers for delivery of proteins and water-insoluble drugs. J. Control. Release 72 (2001) 203-215
133. Choi S., and Kim S.W. Controlled release of insulin from injectable biodegradable triblock copolymer depot in ZDF rats. Pharm. Res. 20 (2003) 2008-2010
134. Choi S., Baudys M., and Kim S.W. Control of blood glucose by novel GLP-1 delivery using biodegradable triblock copolymer of PLGA-PEG-PLGA in type 2 diabetic rats. Pharm. Res. 21 (2004) 827-831