Pegylated polyethylenimine-Fab′ antibody fragment conjugates for targeted gene delivery to human ovarian carcinoma cells

Bioconjugate Chemistry

Volumn 14, Issue 5, 2003, Pages 989-996

  Merdan, Thomas ^a,b   Callahan, Jon ^a   Petersen, Holger ^b   Kunath, Klaus ^b   Bakowsky, Udo ^c   Kopečková, Pavla ^a   Kissel, Thomas ^b   Kopeček, Jindřich ^a  

^a UNIVERSITY OF UTAH   (United States)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c SAARLAND UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; CELL CULTURE; EPITOPES; GENE EXPRESSION; GENE TRANSFER; MOLECULAR BIOLOGY;

ANTIBODY-FRAGMENT; ANTIGEN BINDING; CARCINOMA CELL LINES; DNA COMPLEX; OVARIAN CARCINOMA CELL; PEGYLATED; POLY(ETHYLENIMINE); REPORTER-GENE EXPRESSION; SURFACE ANTIGEN; TARGETED GENE DELIVERY;

DNA;

EPITOPE; ETHIDIUM BROMIDE; HEPARIN; IMMUNOGLOBULIN F(AB) FRAGMENT; POLYETHYLENEIMINE;

ANIMAL CELL; ANTIGEN BINDING; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; CULTURE MEDIUM; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION; GENE TARGETING; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; OVARY CARCINOMA; REPORTER GENE; ZETA POTENTIAL;

ANIMALIA;

EID: 0141681120     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc0340767     Document Type: Article

References (36)

1. Boussif, O., Lezoualc'h, F., Zanta, M. A., Mergny, M. D., Scherman, D., Demeneix, B., and Behr, J. P. (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc. Natl. Acad. Sci. U.S.A. 92, 7297-7301.
2. Ferrari, S., Moro, E., Pettenazzo, A., Behr, J. P., Zacchello, F., and Scarpa, M. (1997) ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo. Gene Ther. 4, 1100-1106.
3. Fischer, D., Bieber, T., Li, Y., Elsasser, H. P., and Kissel, T. (1999) A novel nonviral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity. Pharm. Res. 16, 1273-1279.
4. Tang, M. X., Redemann, C. T., and Szoka, F. C., Jr. (1996) In vitro gene delivery by degraded polyamidoamine dendrimers. Bioconjugate Chem. 7, 703-714.
5. Putnam, D., Gentry, C. A., Pack, D. W., and Langer, R. (2001) Polymer-based gene delivery with low cytotoxicity by a unique balance of side-chain termini. Proc. Natl. Acad. Sci. U.S.A. 98, 1200-1205.
6. Mislick, K. A., and Baldeschwieler, J. D. (1996) Evidence for the role of proteoglycans in cation-mediated gene transfer. Proc. Natl. Acad. Sci. U.S.A. 93, 12349-12354.
7. Chollet, P., Favrot, M. C., Hurbin, A., and Coll, J. L. (2002) Side-effects of a systemic injection of linear polyethylenimine-DNA complexes. J. Gene Med. 4, 84-91.
8. Ogris, M., Brunner, S., Schuller, S., Kircheis, R., and Wagner, E. (1999) PEGylated DNA/transferrin-PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery. Gene Ther. 6, 595-605.
9. Wolfert, M. A., Schacht, E. H., Toncheva, V., Ulbrich, K., Nazarova, O., and Seymour, L. W. (1996) Characterization of vectors for gene therapy formed by self-assembly of DNA with synthetic block copolymers. Hum. Gene Ther. 7, 2123-2133.
10. Kunath, K., von Harpe, A., Petersen, H., Fischer, D., Voigt, K., Kissel, T., and Bickel, U. (2002) The structure of PEG-modified poly(ethylene imines) influences biodistribution and pharmacokinetics of their complexes with NF-kappaB decoy in mice. Pharm. Res. 19, 810-817.
11. Oupický, D., Howard, K. A., Koňak, Č., Dash, P. R., Ulbrich, K., and Seymour, L. W. (2000) Steric stabilization of poly-L-lysine/ DNA complexes by the covalent attachment of semitelechelic poly [N-(2-hydroxypropyl)methacrylamide]. Bioconjugate Chem. 11, 492-501.
12. Kircheis, R., Kichler, A., Wallner, G., Kursa, M., Ogris, M., Felzmann, T., Buchberger, M., and Wagner, E. (1997) Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery. Gene Ther. 4, 409-418.
13. Mislick, K. A., Baldeschwieler, J. D., Kayyem, J. F., and Meade, T. J. (1995) Transfection of folate-polylysine DNA complexes: evidence for lysosomal delivery. Bioconjugate Chem. 6, 512-515.
14. Leamon, C. P., Weigl, D., and Hendren, R. W. (1999) Folate copolymer-mediated transfection of cultured cells. Bioconjugate Chem. 10, 947-957.
15. Erbacher, P., Remy, J. S., and Behr, J. P. (1999) Gene transfer with synthetic virus-like particles via the integrin-mediated endocytosis pathway. Gene Ther. 6, 138-145.
16. Kunath, K., Merdan, T., Hegener, O., Häberlein, H. and Kissel, T. Integrin targeting using RGD-PEI conjugates for in vitro gene transfer. J. Gene Med. 5, 588-599.
17. Choi, Y. H., Liu, F., Choi, J. S., Kim, S. W., and Park, J. S. (1999) Characterization of a targeted gene carrier, lactose-poly(ethylene glycol)-grafted poly-L-lysine and its complex with plasmid DNA. Hum. Gene Ther. 10, 2657-2665.
18. Mahato, R. I., Takemura, S., Akamatsu, K., Nishikawa, M., Takakura, Y., and Hashida, M. (1997) Physicochemical and disposition characteristics of antisense oligonucleotides complexed with glycosylated poly(L-lysine). Biochem. Pharmacol. 53, 887-895.
19. Ferkol, T., Kaetzel, C. S., and Davis, P. B. (1993) Gene transfer into respiratory epithelial cells by targeting the polymeric immunoglobulin receptor. J. Clin. Invest. 92, 2394-2400.
20. Buschle, M., Cotten, M., Kirlappos, H., Mechtler, K., Schaffner, G., Zauner, W., Birnstiel, M. L., and Wagner, E. (1995) Receptor-mediated gene transfer into human T lymphocytes via binding of DNA/CD3 antibody particles to the CD3 T cell receptor complex. Hum. Gene Ther. 6, 753-761.
21. Slobbe, R., Poels, L., ten Dam, G., Boerman, O., Nieland, L., Leunissen, J., Ramaekers, F., and van Eys, G. (1994) Analysis of idiotope structure of ovarian cancer antibodies: recognition of the same epitope by two monoclonal antibodies differing mainly in their heavy chain variable sequences. Clin. Exp. Immunol. 98, 95-103.
22. Omelyanenko, V., Gentry, C., Kopecková, P., and Kopecek, J. (1998) HPMA copolymer-anticancer drug-OV-TL16 antibody conjugates. II. Processing in epithelial ovarian carcinoma cells in vitro. Int. J. Cancer 75, 600-608.
23. Lu, Z. R., Kopecková, P., and Kopecek, J. (1999) Polymerizable Fab′ antibody fragments for targeting of anticancer drugs. Nat. Biotechnol. 17, 1101-1104.
24. Petersen, H., Fechner, P. M., Martín, A. L., Kunath, K., Stolnik, S., Roberts, C. J., Fischer, D., Davies, M. C., and Kissel, T. (2002) Polyethylenimine-graft-poly(ethylene glycol) copolymers: influence of copolymer block structure on DNA complexation and biological activities as gene delivery system. Bioconjugate Chem. 13, 845-854.
25. von Harpe, A., Ph.D. Thesis (2000) Philipps University, Marburg, Germany.
26. Petersen, H., Kunath, K., Martin, A. L., Stolnik, S., Roberts, C. J., Davies, M. C., and Kissel, T. (2002) Star-shaped poly(ethylene glycol)-block-polyethylenimine copolymers enhance DNA condensation of low molecular weight polyethylenimines. Biomacromolecules 3, 926-936.
27. Oberle, V., Bakowsky, U., Zuhorn, I. S., and Hoekstra, D. (2000) Lipoplex formation under equilibrium conditions reveals a three-step mechanism. Biophys. J. 79, 1447-1454.
28. Ogris, M., Steinlein, P., Carotta, S., Brunner, S., and Wagner, E. (2001) DNA/polyethylenimine transfection particles: influence of ligands, polymer size, and PEGylation on internalization and gene expression. AAPS PharmSci. 3, E21.
29. Hill, H. D., Straka, J. G. (1988) Protein determination using bicinchonic acid in the presence of sulfhydryl reagents. Anal. Biochem. 170, 203-208.
30. Mellman, I. (1996) Endocytosis and molecular sorting. Annu. Rev. Cell. Dev. Biol. 12, 575-625.
31. Toncheva, V., Wolfert, M. A., Dash, P. R., Oupický, D., Ulbrich, K., Seymour, L. W., and Schacht, E. H. (1998) Novel vectors for gene delivery formed by self-assembly of DNA with poly(L-lysine) grafted with hydrophilic polymers. Biochim. Biophys. Acta 1380, 354-368.
32. Plank, C., Mechtler, K., Szoka, F. C., Jr., and Wagner, E. (1996) Activation of the complement system by synthetic DNA complexes: a potential barrier for intravenous gene delivery. Hum. Gene Ther. 7, 1437-1446.
33. Dash, P. R., Read, M. L., Barrett, L. B., Wolfert, M. A., and Seymour, L. W. (1999) Factors affecting blood clearance and in vivo distribution of polyelectrolyte complexes for gene delivery. Gene Ther. 6, 643-650.
34. Klemm, A. R., Young, D., and Lloyd, J. B. (1998) Effects of polyethyleneimine on endocytosis and lysosome stability. Biochem. Pharmacol. 56, 41-46.
35. Woodle, M. C., Scaria, P., Ganesh, S., Subramanian, K., Titmas, R., Cheng, C., Yang, J., Pan, Y., Weng, K., Gu, C., and Torkelson, S. (2001) Sterically stabilized polyplex: ligand-mediated activity. J. Controlled Release 74, 309-311.
36. Blessing, T., Kursa, M., Holzhauser, R., Kircheis, R., and Wagner, E. (2001) Different strategies for formation of pegylated EGF-conjugated PEI/DNA complexes for targeted gene delivery. Bioconjugate Chem. 12, 529-537.