Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

Nature

Volumn 411, Issue 6836, 2001, Pages 494-498

  Elbashir, Sayda M ^a   Harborth, Jens ^a   Lendeckel, Winfried ^a   Yalcin, Abdullah ^a   Weber, Klaus ^a   Tuschl, Thomas ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIODIVERSITY; BIOLOGICAL ORGANS; GENES; PLANTS (BOTANY);

GENE SILENCING;

RNA;

NUCLEOTIDE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; GENE CONSTRUCT; GENE EXPRESSION; GENE FUNCTION; GENE REPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; RNA ANALYSIS;

ANIMALS; ANTIGENS, NUCLEAR; BEETLES; CELLS, CULTURED; CNIDARIA; COS CELLS; DROSOPHILA; GENE SILENCING; GENES; GENES, REPORTER; GENETIC TECHNIQUES; HELA CELLS; HUMANS; LAMINS; NUCLEAR MATRIX-ASSOCIATED PROTEINS; NUCLEAR PROTEINS; NUCLEIC ACID HYBRIDIZATION; RNA, DOUBLE-STRANDED; RNA, MESSENGER; VIMENTIN;

ANIMALIA; MAMMALIA;

EID: 0035942736     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35078107     Document Type: Article

References (30)

1. RNA-triggered gene silencing
2. RNA interference 2001
3. Post-transcriptional gene silencing by double-stranded RNA
4. RNA interference and small interfering RNAs
5. A species of small antisense RNA in posttranscriptional gene silencing in plants
6. An RNA-directed nuclease mediates posttranscriptional gene silencing in Drosophila cells
7. RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
8. Role for a bidentate ribonuclease in the initiation step of RNA interference
9. RNA interference is mediated by 21 and 22 nt RNAs
10. dsRNA-mediated gene silencing in cultured Drosophila cells: A tissue culture model for the analysis of RNA interference
11. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways
12. Sensitive assay of RNA interference in Drosophila and Chinese hamster cultured cells using firefly luciferase gene as target
13. Specific interference with gene function by double-stranded RNA in early mouse development
14. Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference
15. Transcriptional and posttranscriptional silencing of rodent alpha(I) collagen by a homologous transcriptionally self-silenced transgene
16. How cells respond to interferons
17. Interactions between double-stranded RNA regulators and the protein kinase DAI
18. Structural requirements of double-stranded RNA for the activation of 2′,5′-oligo(A) polymerase and protein kinase of interferon-treated HeLa cells
19. 5′ A: A novel oligonucleotide synthesized by interferon-treated L cell extracts
20. Inhibition of hepatitis C virus (HCV)-RNA-dependent translation and replication of a chimeric HCV poliovirus using synthetic stabilized ribozymes
21. Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT In vitro
22. Loss of A-type lamin expression compromises nuclear envelope integrity leading to muscular dystrophy
23. RNA-directed DNA methylation
24. Transcriptional silencing and promoter methylation triggered by double-stranded RNA
25. Replicating satellite RNA induces sequence-specific DNA methylation and truncated transcripts in plants
26. CpG methylation, chromatin structure and gene silencing - A three-way connection
27. Induction of nuclear lamins A/C in macrophages in in vitro cultures of rat bone marrow precursor cells and human blood monocytes, and in macrophages elicited in vivo by thioglycollate stimulation
28. Self assembly of NuMA: Multiarm oligomers as structural units of a nuclear lattice
29. Functional anatomy ora dsRNA trigger: Differential requirement for the two trigger strands in RNA Interference