Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage

Genome Biology

Volumn 18, Issue 1, 2017, Pages

  Kulcsár, Péter István ^a,b,c   Tálas, András ^a,d   Huszár, Krisztina ^a,b,e   Ligeti, Zoltán ^a,e   Tóth, Eszter ^a,b   Weinhardt, Nóra ^a,b,c   Fodor, Elfrieda ^b   Welker, Ervin ^a,b  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b INSTITUTE OF BIOCHEMISTRY   (Hungary)

^c UNIVERSITY OF SZEGED   (Hungary)

^d SEMMELWEIS UNIVERSITY   (Hungary)

^e Gene Design Kft   (Hungary)

Author keywords

Cas9; CRISPR; Disruption assay; ESpCas9; Extended sgRNA; HeFm2SpCas9; HeFSpCas9; High fidelity nuclease; Off target; SgRNA; SpCas9 HF1; Truncated sgRNA

Indexed keywords

CASPASE 9; HIGH ENHANCE FIDELITY STREPTOCOCCUS PYOGENE CASPASE 9; NUCLEASE; NUCLEOTIDE; RNA; SINGLE GUIDE RNA; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; DNA; ENDONUCLEASE; PROTEIN BINDING;

ANIMAL CELL; ARTICLE; BASE PAIRING; CONTROLLED STUDY; DNA BINDING; ENZYME SPECIFICITY; EXTENSION GENE; GENE MUTATION; GENETIC VARIATION; HUMAN; HUMAN CELL; MOUSE; NEURO-2A CELL LINE; NONHUMAN; PROPENSITY SCORE; PROTEIN CLEAVAGE; PROTEIN PURIFICATION; PROTEIN TARGETING; RNA TRANSCRIPTION; SENSITIVITY AND SPECIFICITY; WESTERN BLOTTING; WILD TYPE; CHEMISTRY; DNA CLEAVAGE; GENETICS; METABOLISM; MUTATION;

BACTERIAL PROTEINS; DNA; DNA CLEAVAGE; ENDONUCLEASES; MUTATION; PROTEIN BINDING; RNA; SUBSTRATE SPECIFICITY;

EID: 85030690151     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/s13059-017-1318-8     Document Type: Article

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