Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Nature

Volumn 523, Issue 7561, 2015, Pages 481-485

  Kleinstiver, Benjamin P ^a,b   Prew, Michelle S ^a   Tsai, Shengdar Q ^a,b   Topkar, Ved V ^a   Nguyen, Nhu T ^a   Zheng, Zongli ^a,b,c   Gonzales, Andrew P W ^a,b,d   Li, Zhuyun ^a   Peterson, Randall T ^a,b,d   Yeh, Jing Ruey Joanna ^a,b   Aryee, Martin J ^a,b,e   Joung, J Keith ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c KAROLINSKA INSTITUTE   (Sweden)

^d BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^e HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CAS 9 PROTEIN; CRISPR ASSOCIATED PROTEIN; UNCLASSIFIED DRUG;

BACTERIUM; CELLS AND CELL COMPONENTS; CYPRINID; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ENGINEERING; HOMINID; MOLECULAR ANALYSIS; PRECISION;

ANIMAL EMBRYO; ARTICLE; BACTERIAL GENE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; DNA END JOINING REPAIR; ENZYME ENGINEERING; GENE FUNCTION; HUMAN; HUMAN CELL; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN MOTIF; PROTOSPACER ADJACENT MOTIF; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PYOGENES; STREPTOCOCCUS THERMOPHILUS; U2OS CELL LINE; WILD TYPE; AMINO ACID SUBSTITUTION; ANIMAL; CELL LINE; CRISPR CAS SYSTEM; DIRECTED MOLECULAR EVOLUTION; EMBRYOLOGY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; GENOME; METABOLISM; MUTATION; NUCLEOTIDE MOTIF; PROCEDURES; PROTEIN ENGINEERING; ZEBRA FISH;

BACTERIA (MICROORGANISMS); DANIO RERIO; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PYOGENES; STREPTOCOCCUS THERMOPHILUS;

AMINO ACID SUBSTITUTION; ANIMALS; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; DIRECTED MOLECULAR EVOLUTION; GENOME; HUMANS; MUTATION; NUCLEOTIDE MOTIFS; PROTEIN ENGINEERING; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PYOGENES; STREPTOCOCCUS THERMOPHILUS; SUBSTRATE SPECIFICITY; ZEBRAFISH;

EID: 84937908208     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature14592     Document Type: Article

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