Sequence- and structure-specific RNA processing by a CRISPR endonuclease

Science

Volumn 329, Issue 5997, 2010, Pages 1355-1358

  Haurwitz, Rachel E ^a   Jinek, Martin ^a   Wiedenheft, Blake ^a   Zhou, Kaihong ^a   Doudna, Jennifer A ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED RNA; ENDONUCLEASE; HISTIDINE; PHOSPHATE; RIBONUCLEASE; RNA; SERINE; SHORT HAIRPIN RNA; SINGLE STRANDED RNA;

BACTERIUM; CONSERVATION GENETICS; CRYSTAL STRUCTURE; GENETIC ANALYSIS; GENETIC STRUCTURE; IMMUNE SYSTEM; PHOSPHATE; PHYLOGENETICS;

ARTICLE; BASE PAIRING; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME SUBSTRATE; GENE LOCUS; HYDROGEN BOND; IMMUNE SYSTEM; MOLECULAR RECOGNITION; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; PYROCOCCUS FURIOSUS; RNA BINDING; RNA CONFORMATION; RNA PROCESSING; RNA SEQUENCE; RNA STRUCTURE; RNA TRANSCRIPTION; SEQUENCE ANALYSIS; STATIC ELECTRICITY; STREPTOCOCCUS THERMOPHILUS; STRUCTURE ANALYSIS; THERMUS THERMOPHILUS;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BASE PAIRING; BASE SEQUENCE; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ENDORIBONUCLEASES; GENES, BACTERIAL; HYDROGEN BONDING; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS AERUGINOSA; REPETITIVE SEQUENCES, NUCLEIC ACID; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, BACTERIAL; STATIC ELECTRICITY;

PSEUDOMONAS AERUGINOSA;

EID: 77956498326     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1192272     Document Type: Article

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25. We thank W. Westphal for help with purification of Csy4 constructs; J. van der Oost for discussion; J. Doudna Cate and members of the Doudna laboratory for critical reading of the manuscript; and C. Ralston and J. Holton (Beamlines 8.2.2 and 8.3.1, Advanced Light Source, Lawrence Berkeley National Laboratory) and S. Coyle for assistance with X-ray data collection. R.E.H. is supported by the U.S. NIH training grant 5 T32 GM08295. M.J. is supported by a Human Frontier Science Program Long-Term Fellowship. B.W. is a Howard Hughes Medical Institute Fellow of the Life Sciences Research Foundation. This work was supported in part by grants from NSF and the Bill and Melinda Gates Foundation. J.A.D. is a Howard Hughes Medical Institute Investigator. Coordinates and structure factors for the Csy4-crRNA complex have been deposited in the Protein Data Bank under accession codes 2xli, 2xlj, and 2xlk. The authors have filed a related patent.