Rational design of a split-Cas9 enzyme complex

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 10, 2015, Pages 2984-2989

  Wright, Addison V ^a   Sternberg, Samuel H ^a   Taylor, David W ^a   Staahl, Brett T ^a   Bardales, Jorge A ^a   Kornfeld, Jack E ^a   Doudna, Jennifer A ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

CRISPR Cas9; Genome engineering; Split enzyme

Indexed keywords

ENZYME; GUIDE RNA; POLYPEPTIDE; SPLIT CAS9 ENZYME COMPLEX; UNCLASSIFIED DRUG; DNA;

ALPHA HELIX; ANIMAL CELL; ARTICLE; CATALYSIS; DIMERIZATION; DNA CLEAVAGE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; GENOME; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTERACTION; VALIDATION PROCESS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONFORMATION; ENZYMOLOGY; GEL MOBILITY SHIFT ASSAY; GENETIC TRANSCRIPTION; HYDROLYSIS; METABOLISM; STREPTOCOCCUS PYOGENES; VALIDATION STUDY;

BACTERIA (MICROORGANISMS); STREPTOCOCCUS PYOGENES;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DNA; ELECTROPHORETIC MOBILITY SHIFT ASSAY; HYDROLYSIS; NUCLEIC ACID CONFORMATION; STREPTOCOCCUS PYOGENES; TRANSCRIPTION, GENETIC;

EID: 84924322574     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1501698112     Document Type: Article

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