Gene editing in mouse zygotes using the CRISPR/Cas9 system

Methods

Volumn 121-122, Issue , 2017, Pages 55-67

  Wefers, Benedikt ^a,b   Bashir, Sanum ^c,d   Rossius, Jana ^c   Wurst, Wolfgang ^a,b,e,f   Kühn, Ralf ^c,d  

^a GERMAN CENTER FOR NEURODEGENERATIVE DISEASES DZNE   (Germany)

^b INSTITUTE OF EPIDEMIOLOGY   (Germany)

^c MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^d BERLIN INSTITUTE OF HEALTH   (Germany)

^e TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^f Germany 3 Munich Cluster for Systems Neurology   (Germany)

Author keywords

Cas9; CRISPR; Gene editing; Mouse; Zygotes

Indexed keywords

CASPASE 9; CRISPR ASSOCIATED PROTEIN; GUIDE RNA; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; DNA; ENDONUCLEASE; GT(ROSA)26SOR NON-CODING RNA, MOUSE; UNTRANSLATED RNA;

ALLELE; ARTICLE; BACKCROSSING; BLASTOCYST; CONTROLLED STUDY; ELECTROPORATION; EMBRYO; FEMALE; GENE; GENE EDITING; GENE INACTIVATION; GENE ISOLATION; GENE LOCUS; GENE MUTATION; GENE SEQUENCE; GENE TARGETING; GENOTYPE; MALE; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; ROSA26 GENE; SANGER SEQUENCING; ZYGOTE; ANIMAL; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CRISPR CAS SYSTEM; CYTOLOGY; DNA END JOINING REPAIR; DOUBLE STRANDED DNA BREAK; GENE KNOCK-IN; GENE KNOCKOUT; GENE TRANSFER; GENETICS; GENOME; METABOLISM; MICROINJECTION; NEWBORN; PROCEDURES; RECOMBINATION REPAIR; TRANSGENIC MOUSE;

ANIMALS; ANIMALS, NEWBORN; BACTERIAL PROTEINS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; DNA; DNA BREAKS, DOUBLE-STRANDED; DNA END-JOINING REPAIR; ELECTROPORATION; ENDONUCLEASES; GENE EDITING; GENE KNOCK-IN TECHNIQUES; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; GENE TRANSFER TECHNIQUES; GENOME; MICE; MICE, TRANSGENIC; MICROINJECTIONS; RECOMBINATIONAL DNA REPAIR; RNA, GUIDE; RNA, UNTRANSLATED; ZYGOTE;

EID: 85020871308     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2017.02.008     Document Type: Article

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