CRISP-ID: Decoding CRISPR mediated indels by Sanger sequencing

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Dehairs, Jonas ^a   Talebi, Ali ^a   Cherifi, Yacine ^b   Swinnen, Johannes V ^a  

^a University of Leuven   (Belgium)

^b GenOway   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENOTYPE; HUMAN; HUMAN EXPERIMENT; INDEL MUTATION; VELOCITY; ALGORITHM; CRISPR CAS SYSTEM; DNA SEQUENCE; GENE EDITING; INTERNET; PROCEDURES;

ALGORITHMS; CRISPR-CAS SYSTEMS; GENE EDITING; INDEL MUTATION; INTERNET; SEQUENCE ANALYSIS, DNA;

EID: 84976907434     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep28973     Document Type: Article

References (11)

1. Urnov, F. D. et al. Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature 435, 646-651 (2005).
2. Cermak, T. et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res. 39, e82 (2011).
3. Mali, P. et al. RNA-Guided Human Genome Engineering via Cas9. Science 339, 823-826 (2013).
4. Wang, H. et al. One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering. Cell 153, 910-918 (2013).
5. Hwang, W. Y. et al. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat. Biotechnol. 31, 227-229 (2013).
6. Sander, J. D., Joung, J. K. CRISPR-Cas systems for editing, regulating and targeting genomes. Nat. Biotechnol. 32, 347-355 (2014).
7. Dmitriev, D. A., Rakitov, R. A. Decoding of Superimposed Traces Produced by Direct Sequencing of Heterozygous Indels. PLoS Comput Biol. 4, 1-10 (2008).
8. Zhidkov, I., Cohen, R., Geifman, N., Mishmar, D., Rubin, E. CHILD: a new tool for detecting low-abundance insertions and deletions in standard sequence traces. Nucleic Acids Res. 39, e47 (2011).
9. Chang, C.-T. et al. Mixed Sequence Reader: A Program for Analyzing DNA Sequences with Heterozygous Base Calling. Sci. World J. 2012 (2012).
10. Prli, A. et al. BioJava: an open-source framework for bioinformatics in 2012. Bioinforma. Oxf. Engl. 28, 2693-2695 (2012).
11. Ran, F. A. et al. Genome engineering using the CRISPR-Cas9 system. Nat. Protoc. 8, 2281-2308 (2013).