Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes

BMC Biotechnology

Volumn 16, Issue 1, 2016, Pages

  Chu, Van Trung ^a   Weber, Timm ^a   Graf, Robin ^a   Sommermann, Thomas ^a   Petsch, Kerstin ^a   Sack, Ulrike ^b   Volchkov, Pavel ^c   Rajewsky, Klaus ^a   Kühn, Ralf ^a,d  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^b BAYER PHARMA AG   (Germany)

^c HARVARD UNIVERSITY   (United States)

^d BERLIN INSTITUTE OF HEALTH   (Germany)

Author keywords

Cas9; CRISPR; Knock in mice; Rosa26; Zygotes

Indexed keywords

MAMMALS;

CAS9; CRISPR; KNOCK-IN MICE; ROSA26; ZYGOTES;

GENES;

CAS9 PROTEIN; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CRE RECOMBINASE; MESSENGER RNA; UNCLASSIFIED DRUG; GT(ROSA)26SOR NON-CODING RNA, MOUSE; UNTRANSLATED RNA;

ANIMAL TISSUE; ARTICLE; B LYMPHOCYTE; CAS9 GENE; CONTROLLED STUDY; CRISPR CAS SYSTEM; FEMALE; GENE FREQUENCY; GENE FUNCTION; GENE IDENTIFICATION; GENE INACTIVATION; GENE INSERTION; GENE TARGETING; IN VIVO STUDY; LOXP SITE; MALE; MOLECULAR DYNAMICS; MOUSE; NONHUMAN; TRANSGENE; ZYGOTE; ANIMAL; C57BL MOUSE; GENETICS; MAMMALIAN EMBRYO; MICROINJECTION; MOLECULAR CLONING; PROCEDURES;

ANIMALS; CLONING, MOLECULAR; CRISPR-CAS SYSTEMS; EMBRYO, MAMMALIAN; GENE KNOCK-IN TECHNIQUES; MICE; MICE, INBRED C57BL; MICROINJECTIONS; RNA, UNTRANSLATED;

EID: 84954439228     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/s12896-016-0234-4     Document Type: Article

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