Utilization of the CRISPR/Cas9 system for the efficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and Fokl-dCas9

Experimental Animals

Volumn 65, Issue 3, 2016, Pages 275-283

  Terao, Miho ^a   Tamano, Moe ^a   Hara, Satoshi ^a   Kato, Tomoko ^a   Kinoshita, Masato ^b   Takada, Shuji ^a  

^a NATIONAL RESEARCH INSTITUTE FOR CHILD HEALTH AND DEVELOPMENT   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

CRISPR Cas9; CrRNA; Genome editing; Mouse

Indexed keywords

CAS9 NICKASE PROTEIN; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CRISPR ASSOCIATED PROTEIN; CRISPR RNA; FOKI DCAS9 PROTEIN; PEPTIDES AND PROTEINS; RNA; TRANS ACTIVATING CRRNA; UNCLASSIFIED DRUG; DEOXYRIBONUCLEASE I;

ARTICLE; CRISPR CAS SYSTEM; GENE MUTATION; GENE SEQUENCE; GENOTYPE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN DENATURATION; ANIMAL; GENE EDITING; GENETICS; MICROINJECTION; MUTANT MOUSE STRAIN; PROCEDURES; TRANSCRIPTION INITIATION; TRANSGENIC ANIMAL; ZYGOTE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASE I; GENE EDITING; MICE, MUTANT STRAINS; MICROINJECTIONS; RNA; TRANSCRIPTIONAL ACTIVATION; ZYGOTE;

EID: 84979780028     PISSN: 13411357     EISSN: None     Source Type: Journal    
DOI: 10.1538/expanim.15-0116     Document Type: Article

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