-
1
-
-
84865070369
-
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity
-
Jinek,M., Chylinski,K., Fonfara,I., Hauer,M., Doudna,J.A. and Charpentier,E. (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 337, 816-821.
-
(2012)
Science
, vol.337
, pp. 816-821
-
-
Jinek, M.1
Chylinski, K.2
Fonfara, I.3
Hauer, M.4
Doudna, J.A.5
Charpentier, E.6
-
2
-
-
84873729095
-
Multiplex genome engineering using CRISPR/Cas systems
-
Cong,L., Ran,F.A., Cox,D., Lin,S., Barretto,R., Habib,N., Hsu,P.D., Wu,X., Jiang,W., Marraffini,L.A. et al. (2013) Multiplex genome engineering using CRISPR/Cas systems. Science, 339, 819-823.
-
(2013)
Science
, vol.339
, pp. 819-823
-
-
Cong, L.1
Ran, F.A.2
Cox, D.3
Lin, S.4
Barretto, R.5
Habib, N.6
Hsu, P.D.7
Wu, X.8
Jiang, W.9
Marraffini, L.A.10
-
3
-
-
84884160273
-
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
-
Mali,P., Aach,J., Stranges,P.B., Esvelt,K.M., Moosburner,M., Kosuri,S., Yang,L. and Church,G.M. (2013) CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat. Biotechnol., 31, 833-838.
-
(2013)
Nat. Biotechnol
, vol.31
, pp. 833-838
-
-
Mali, P.1
Aach, J.2
Stranges, P.B.3
Esvelt, K.M.4
Moosburner, M.5
Kosuri, S.6
Yang, L.7
Church, G.M.8
-
4
-
-
84880570576
-
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
-
Fu,Y., Foden,J.A., Khayter,C., Maeder,M.L., Reyon,D., Joung,J.K. and Sander,J.D. (2013) High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat. Biotechnol., 31, 822-826.
-
(2013)
Nat. Biotechnol
, vol.31
, pp. 822-826
-
-
Fu, Y.1
Foden, J.A.2
Khayter, C.3
Maeder, M.L.4
Reyon, D.5
Joung, J.K.6
Sander, J.D.7
-
5
-
-
84884165315
-
DNA targeting specificity of RNA-guided Cas9 nucleases
-
Hsu,P.D., Scott,D.A., Weinstein,J.A., Ran,F.A., Konermann,S., Agarwala,V., Li,Y., Fine,E.J., Wu,X., Shalem,O. et al. (2013) DNA targeting specificity of RNA-guided Cas9 nucleases. Nat. Biotechnol., 31, 827-832.
-
(2013)
Nat. Biotechnol
, vol.31
, pp. 827-832
-
-
Hsu, P.D.1
Scott, D.A.2
Weinstein, J.A.3
Ran, F.A.4
Konermann, S.5
Agarwala, V.6
Li, Y.7
Fine, E.J.8
Wu, X.9
Shalem, O.10
-
6
-
-
84884155038
-
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
-
Pattanayak,V., Lin,S., Guilinger,J.P., Ma,E., Doudna,J.A. and Liu,D.R. (2013) High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat. Biotechnol., 31, 839-843.
-
(2013)
Nat. Biotechnol
, vol.31
, pp. 839-843
-
-
Pattanayak, V.1
Lin, S.2
Guilinger, J.P.3
Ma, E.4
Doudna, J.A.5
Liu, D.R.6
-
7
-
-
84923221641
-
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
-
Wang,X., Wang,Y., Wu,X., Wang,J., Wang,Y., Qiu,Z., Chang,T., Huang,H., Lin,R.-J. and Yee,J.-K. (2015) Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors. Nat. Biotechnol., 33, 175-178.
-
(2015)
Nat. Biotechnol
, vol.33
, pp. 175-178
-
-
Wang, X.1
Wang, Y.2
Wu, X.3
Wang, J.4
Wang, Y.5
Qiu, Z.6
Chang, T.7
Huang, H.8
Lin, R.-J.9
Yee, J.-K.10
-
8
-
-
84884950106
-
CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity
-
Cradick,T.J., Fine,E.J., Antico,C.J. and Bao,G. (2013) CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity. Nucleic Acids Res., 41, 9584-9592.
-
(2013)
Nucleic Acids Res
, vol.41
, pp. 9584-9592
-
-
Cradick, T.J.1
Fine, E.J.2
Antico, C.J.3
Bao, G.4
-
9
-
-
84923846574
-
Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells
-
Kim,D., Bae,S., Park,J., Kim,E., Kim,S., Yu,H.R., Hwang,J., Kim,J.-I. and Kim,J.-S. (2015) Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods, 12, 237-243.
-
(2015)
Nat. Methods
, vol.12
, pp. 237-243
-
-
Kim, D.1
Bae, S.2
Park, J.3
Kim, E.4
Kim, S.5
Yu, H.R.6
Hwang, J.7
Kim, J.-I.8
Kim, J.-S.9
-
10
-
-
84923266604
-
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
-
Tsai,S.Q., Zheng,Z., Nguyen,N.T., Liebers,M., Topkar,V.V., Thapar,V., Wyvekens,N., Khayter,C., Iafrate,A.J., Le,L.P. et al. (2015) GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Nat. Biotechnol., 33, 187-197.
-
(2015)
Nat. Biotechnol
, vol.33
, pp. 187-197
-
-
Tsai, S.Q.1
Zheng, Z.2
Nguyen, N.T.3
Liebers, M.4
Topkar, V.V.5
Thapar, V.6
Wyvekens, N.7
Khayter, C.8
Iafrate, A.J.9
Le, L.P.10
-
11
-
-
84902141229
-
Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs
-
Gagnon,J.A., Valen,E., Thyme,S.B., Huang,P., Ahkmetova,L., Pauli,A., Montague,T.G., Zimmerman,S., Richter,C. and Schier,A.F. (2014) Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. PLoS One, 9, e98186.
-
(2014)
PLoS One
, vol.9
, pp. e98186
-
-
Gagnon, J.A.1
Valen, E.2
Thyme, S.B.3
Huang, P.4
Ahkmetova, L.5
Pauli, A.6
Montague, T.G.7
Zimmerman, S.8
Richter, C.9
Schier, A.F.10
-
12
-
-
84959123021
-
CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo
-
Moreno-Mateos,M.A., Vejnar,C.E., Beaudoin,J.-D., Fernandez,J.P., Mis,E.K., Khokha,M.K. and Giraldez,A.J. (2015) CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo. Nat. Methods, 12, 982-988.
-
(2015)
Nat. Methods
, vol.12
, pp. 982-988
-
-
Moreno-Mateos, M.A.1
Vejnar, C.E.2
Beaudoin, J.-D.3
Fernandez, J.P.4
Mis, E.K.5
Khokha, M.K.6
Giraldez, A.J.7
-
13
-
-
84957605863
-
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
-
Doench,J.G., Fusi,N., Sullender,M., Hegde,M., Vaimberg,E.W., Donovan,K.F., Smith,I., Tothova,Z., Wilen,C., Orchard,R. et al. (2016) Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat. Biotechnol., 34, 184-191.
-
(2016)
Nat. Biotechnol
, vol.34
, pp. 184-191
-
-
Doench, J.G.1
Fusi, N.2
Sullender, M.3
Hegde, M.4
Vaimberg, E.W.5
Donovan, K.F.6
Smith, I.7
Tothova, Z.8
Wilen, C.9
Orchard, R.10
-
14
-
-
84921540377
-
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation
-
Doench,J.G., Hartenian,E., Graham,D.B., Tothova,Z., Hegde,M., Smith,I., Sullender,M., Ebert,B.L., Xavier,R.J. and Root,D.E. (2014) Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat. Biotechnol., 32, 1262-1267.
-
(2014)
Nat. Biotechnol
, vol.32
, pp. 1262-1267
-
-
Doench, J.G.1
Hartenian, E.2
Graham, D.B.3
Tothova, Z.4
Hegde, M.5
Smith, I.6
Sullender, M.7
Ebert, B.L.8
Xavier, R.J.9
Root, D.E.10
-
15
-
-
84938829555
-
Sequence determinants of improved CRISPR sgRNA design
-
Xu,H., Xiao,T., Chen,C.-H., Li,W., Meyer,C.A., Wu,Q., Wu,D., Cong,L., Zhang,F., Liu,J.S. et al. (2015) Sequence determinants of improved CRISPR sgRNA design. Genome Res., 25, 1147-1157.
-
(2015)
Genome Res
, vol.25
, pp. 1147-1157
-
-
Xu, H.1
Xiao, T.2
Chen, C.-H.3
Li, W.4
Meyer, C.A.5
Wu, Q.6
Wu, D.7
Cong, L.8
Zhang, F.9
Liu, J.S.10
-
16
-
-
84940501210
-
Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach
-
Chari,R., Mali,P., Moosburner,M. and Church,G. M. (2015). Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Nat. Methods, 12, 823-826.
-
(2015)
Nat. Methods
, vol.12
, pp. 823-826
-
-
Chari, R.1
Mali, P.2
Moosburner, M.3
Church, G. M.4
-
17
-
-
84975678715
-
Cpf1 is a single RNA-Guided endonuclease of a class 2 CRISPR-Cas system
-
Zetsche,B., Gootenberg,J.S., Abudayyeh,O.O., Slaymaker,I.M., Makarova,K.S., Essletzbichler,P., Volz,S.E., Joung,J., van der Oost,J., Regev,A. et al. (2015) Cpf1 is a single RNA-Guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163, 759-771.
-
(2015)
Cell
, vol.163
, pp. 759-771
-
-
Zetsche, B.1
Gootenberg, J.S.2
Abudayyeh, O.O.3
Slaymaker, I.M.4
Makarova, K.S.5
Essletzbichler, P.6
Volz, S.E.7
Joung, J.8
van der Oost, J.9
Regev, A.10
-
18
-
-
84963941043
-
High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects
-
Kleinstiver,B.P., Pattanayak,V., Prew,M.S., Tsai,S.Q., Nguyen,N.T., Zheng,Z. and Keith Joung,J. (2016) High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature, 529, 490-495.
-
(2016)
Nature
, vol.529
, pp. 490-495
-
-
Kleinstiver, B.P.1
Pattanayak, V.2
Prew, M.S.3
Tsai, S.Q.4
Nguyen, N.T.5
Zheng, Z.6
Keith Joung, J.7
-
19
-
-
84937908208
-
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
-
Kleinstiver,B.P., Prew,M.S., Tsai,S.Q., Topkar,V.V., Nguyen,N.T., Zheng,Z., Gonzales,A.P.W., Li,Z., Peterson,R.T., Yeh,J.-R.J. et al. (2015) Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature, 523, 481-485.
-
(2015)
Nature
, vol.523
, pp. 481-485
-
-
Kleinstiver, B.P.1
Prew, M.S.2
Tsai, S.Q.3
Topkar, V.V.4
Nguyen, N.T.5
Zheng, Z.6
Gonzales, A.P.W.7
Li, Z.8
Peterson, R.T.9
Yeh, J.-R.J.10
-
20
-
-
84896929630
-
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
-
Fu,Y., Sander,J.D., Reyon,D., Cascio,V.M. and Joung,J.K. (2014) Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat. Biotechnol., 32, 279-284.
-
(2014)
Nat. Biotechnol
, vol.32
, pp. 279-284
-
-
Fu, Y.1
Sander, J.D.2
Reyon, D.3
Cascio, V.M.4
Joung, J.K.5
-
21
-
-
84904813279
-
CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing
-
Montague,T.G., Cruz,J.M., Gagnon,J.A., Church,G.M. and Valen,E. (2014) CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing. Nucleic Acids Res., 42, W401-W407.
-
(2014)
Nucleic Acids Res
, vol.42
, pp. W401-W407
-
-
Montague, T.G.1
Cruz, J.M.2
Gagnon, J.A.3
Church, G.M.4
Valen, E.5
-
22
-
-
0036079158
-
The human genome browser at UCSC
-
Kent,W.J., Sugnet,C.W., Furey,T.S., Roskin,K.M., Pringle,T.H., Zahler,A.M. and Haussler,D. (2002) The human genome browser at UCSC. Genome Res., 12, 996-1006.
-
(2002)
Genome Res
, vol.12
, pp. 996-1006
-
-
Kent, W.J.1
Sugnet, C.W.2
Furey, T.S.3
Roskin, K.M.4
Pringle, T.H.5
Zahler, A.M.6
Haussler, D.7
-
23
-
-
84882986957
-
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
-
Bikard,D., Jiang,W., Samai,P., Hochschild,A., Zhang,F. and Marraffini,L.A. (2013) Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res., 41, 7429-7437.
-
(2013)
Nucleic Acids Res
, vol.41
, pp. 7429-7437
-
-
Bikard, D.1
Jiang, W.2
Samai, P.3
Hochschild, A.4
Zhang, F.5
Marraffini, L.A.6
-
24
-
-
84874687019
-
Repurposing CRISPR as an RNA-Guided platform for sequence-specific control of gene expression
-
Qi,L.S., Larson,M.H., Gilbert,L.A., Doudna,J.A., Weissman,J.S., Arkin,A.P. and Lim,W.A. (2013) Repurposing CRISPR as an RNA-Guided platform for sequence-specific control of gene expression. Cell, 152, 1173-1183.
-
(2013)
Cell
, vol.152
, pp. 1173-1183
-
-
Qi, L.S.1
Larson, M.H.2
Gilbert, L.A.3
Doudna, J.A.4
Weissman, J.S.5
Arkin, A.P.6
Lim, W.A.7
-
25
-
-
84929135130
-
Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers
-
Hilton,I.B., D'Ippolito,A.M., Vockley,C.M., Thakore,P.I., Crawford,G.E., Reddy,T.E. and Gersbach,C.A. (2015) Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat. Biotechnol., 33, 510-517.
-
(2015)
Nat. Biotechnol
, vol.33
, pp. 510-517
-
-
Hilton, I.B.1
D'Ippolito, A.M.2
Vockley, C.M.3
Thakore, P.I.4
Crawford, G.E.5
Reddy, T.E.6
Gersbach, C.A.7
-
26
-
-
62349130698
-
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
-
Langmead,B., Trapnell,C., Pop,M. and Salzberg,S. (2009) Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol., 10, R25.
-
(2009)
Genome Biol
, vol.10
, pp. R25
-
-
Langmead, B.1
Trapnell, C.2
Pop, M.3
Salzberg, S.4
-
27
-
-
84865022311
-
Primer3-new capabilities and interfaces
-
Untergasser,A., Cutcutache,I., Koressaar,T., Ye,J., Faircloth,B.C., Remm,M. and Rozen,S.G. (2012) Primer3-new capabilities and interfaces. Nucleic Acids Res., 40, e115.
-
(2012)
Nucleic Acids Res
, vol.40
, pp. e115
-
-
Untergasser, A.1
Cutcutache, I.2
Koressaar, T.3
Ye, J.4
Faircloth, B.C.5
Remm, M.6
Rozen, S.G.7
-
28
-
-
84895832944
-
Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems
-
Fonfara,I., Le Rhun,A., Chylinski,K., Makarova,K.S., Lécrivain,A.-L., Bzdrenga,J., Koonin,E.V. and Charpentier,E. (2014) Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems. Nucleic Acids Res., 42, 2577-2590.
-
(2014)
Nucleic Acids Res
, vol.42
, pp. 2577-2590
-
-
Fonfara, I.1
Le Rhun, A.2
Chylinski, K.3
Makarova, K.S.4
Lécrivain, A.-L.5
Bzdrenga, J.6
Koonin, E.V.7
Charpentier, E.8
-
29
-
-
0022422660
-
Nomenclature for incompletely specified bases in nucleic acid sequences: recommendations 1984
-
Cornish-Bowden,A. (1985) Nomenclature for incompletely specified bases in nucleic acid sequences: recommendations 1984. Nucleic Acids Res., 13, 3021-3030.
-
(1985)
Nucleic Acids Res
, vol.13
, pp. 3021-3030
-
-
Cornish-Bowden, A.1
-
30
-
-
84892749369
-
Genetic screens in human cells using the CRISPR-Cas9 system
-
Wang,T., Wei,J.J., Sabatini,D.M. and Lander,E.S. (2014) Genetic screens in human cells using the CRISPR-Cas9 system. Science, 343, 80-84.
-
(2014)
Science
, vol.343
, pp. 80-84
-
-
Wang, T.1
Wei, J.J.2
Sabatini, D.M.3
Lander, E.S.4
-
31
-
-
84974644123
-
Internal guide RNA interactions interfere with Cas9-mediated cleavage
-
Thyme,S.B., Akhmetova,L., Montague,T.G., Valen,E. and Schier,A.F. (2016) Internal guide RNA interactions interfere with Cas9-mediated cleavage. Nat. Commun., 7, 11750.
-
(2016)
Nat. Commun
, vol.7
, pp. 11750
-
-
Thyme, S.B.1
Akhmetova, L.2
Montague, T.G.3
Valen, E.4
Schier, A.F.5
-
32
-
-
84884288934
-
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
-
Ran,F.A., Hsu,P.D., Lin,C.-Y., Gootenberg,J.S., Konermann,S., Trevino,A.E., Scott,D.A., Inoue,A., Matoba,S., Zhang,Y. et al. (2013) Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell, 154, 1380-1389.
-
(2013)
Cell
, vol.154
, pp. 1380-1389
-
-
Ran, F.A.1
Hsu, P.D.2
Lin, C.-Y.3
Gootenberg, J.S.4
Konermann, S.5
Trevino, A.E.6
Scott, D.A.7
Inoue, A.8
Matoba, S.9
Zhang, Y.10
|