Re-engineered RNA-Guided FokI-Nucleases for Improved Genome Editing in Human Cells

Molecular Therapy

Volumn 25, Issue 2, 2017, Pages 342-355

  Havlicek, Steven ^a   Shen, Yang ^a   Alpagu, Yunus ^b   Bruntraeger, Michaela B ^a   Zufir, Nurdiana B M ^a   Phuah, Zhi Yi ^a   Fu, Zhiyan ^a   Dunn, Norris R ^b   Stanton, Lawrence W ^a,c,d  

^a GENOME INSTITUTE OF SINGAPORE   (Singapore)

^b INSTITUTE OF MEDICAL BIOLOGY   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

Author keywords

CRISPR Cas9; FokI; gene editing; RNA guided FokI nuclease

Indexed keywords

NUCLEASE; RNA GUIDED FOKI NUCLEASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; ENDODEOXYRIBONUCLEASE FOKI; ENDONUCLEASE; GUIDE RNA; PROTEIN BINDING; REGULATORY FACTOR X TRANSCRIPTION FACTOR; RFX6 PROTEIN, HUMAN; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

ARTICLE; CHIMERA; CRISPR CAS SYSTEM; ENZYME ENGINEERING; FEASIBILITY STUDY; GENE EDITING; GENE TARGETING; GENETIC ENGINEERING; HUMAN; HUMAN CELL; ORTHOLOGY; STAPHYLOCOCCUS AUREUS; BINDING SITE; BIOLOGICAL MODEL; CHEMISTRY; GENETIC VARIATION; GENETICS; METABOLISM; MUTATION; NUCLEOTIDE SEQUENCE; PLURIPOTENT STEM CELL; PROTEIN ENGINEERING; PROTEIN MULTIMERIZATION;

BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; ENDONUCLEASES; GENE EDITING; GENETIC VARIATION; HUMANS; MODELS, BIOLOGICAL; MUTATION; PLURIPOTENT STEM CELLS; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN MULTIMERIZATION; REGULATORY FACTOR X TRANSCRIPTION FACTORS; RNA, GUIDE;

EID: 85016316834     PISSN: 15250016     EISSN: 15250024     Source Type: Journal    
DOI: 10.1016/j.ymthe.2016.11.007     Document Type: Article

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