An iCRISPR platform for rapid, multiplexable, and inducible genome editing in human pluripotent stem cells

Cell Stem Cell

Volumn 15, Issue 2, 2014, Pages 215-226

  González, Federico ^a   Zhu, Zengrong ^a   Shi, Zhong Dong ^a   Lelli, Katherine ^a   Verma, Nipun ^a,b   Li, Qing V ^a   Huangfu, Danwei ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b Weill Cornell Medical Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SINGLE STRANDED DNA; GUIDE RNA; NEUROGENIN 3; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; TRANSCRIPTION FACTOR GATA 4; TRANSCRIPTION FACTOR GATA 6;

ALLELE; BIOLOGICAL MODEL; CELL DIFFERENTIATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CYTOLOGY; GENE INACTIVATION; GENE TARGETING; GENE VECTOR; GENETIC ENGINEERING; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GENETICS; GENOME; HETEROZYGOTE; HOMOZYGOTE; HUMAN; INDUCED PLURIPOTENT STEM CELL; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; PHENOTYPE; PROCEDURES; SEQUENCE HOMOLOGY; ARTICLE; CLONAL CELL LINE; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA CLEAVAGE; DNA SEQUENCE; ELECTROPORATION; GENE FUNCTION; GENE INTERACTION; GENETIC ANALYSIS; GENETIC MANIPULATION; GENETIC SUSCEPTIBILITY; HUMAN CELL; INDEL MUTATION; INTRON; LOSS OF FUNCTION MUTATION; MUTATION RATE; NONHUMAN; PLEIOTROPY; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROMOTER REGION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RESTRICTION SITE; SINGLE NUCLEOTIDE POLYMORPHISM;

ALLELES; BASE SEQUENCE; CELL DIFFERENTIATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DNA, SINGLE-STRANDED; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; GENETIC ENGINEERING; GENETIC VECTORS; GENOME; HETEROZYGOTE; HOMOZYGOTE; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 84905905141     PISSN: 19345909     EISSN: 18759777     Source Type: Journal    
DOI: 10.1016/j.stem.2014.05.018     Document Type: Article

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