Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

Nature Biotechnology

Volumn 33, Issue 12, 2015, Pages 1293-1298

  Kleinstiver, Benjamin P ^a,b   Prew, Michelle S ^a   Tsai, Shengdar Q ^a,b   Nguyen, Nhu T ^a   Topkar, Ved V ^a   Zheng, Zongli ^c   Joung, J Keith ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c CITY UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

DNA SEQUENCES; GENES; MOLECULAR BIOLOGY; NUCLEIC ACIDS;

HUMAN CELLS; MOLECULAR EVOLUTION; ORTHOLOGS; RECOGNITION SPECIFICITY; STAPHYLOCOCCUS AUREUS; STRUCTURAL INFORMATION; TARGET SITES; WILD TYPES;

BACTERIA;

GUIDE RNA;

ARTICLE; BACTERIAL CELL; BINDING SITE; COMPARATIVE STUDY; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; DNA BASE COMPOSITION; DNA SEQUENCE; HUMAN; MISSENSE MUTATION; MOLECULAR EVOLUTION; MUTAGENESIS; NONHUMAN; ORTHOLOGY; POSITION WEIGHT MATRIX; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS;

EID: 84949791988     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3404     Document Type: Article

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